1<!DOCTYPE html> 2<html> 3 4 <head> 5 6 7 <meta charset="utf-8"> 8 <meta name="viewport" content="width=device-width, initial-scale=1, shrink-to-fit=no"> 9 <meta name="description" content="Laboratory Website!"> 10 <meta name="author" content="Hani N. 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class="nav-item"><a class="nav-link js-scroll-trigger" href="https://lbr-lab.com/#funding">Funding</a></li> 73 <li class="nav-item"><a class="nav-link js-scroll-trigger" href="https://lbr-lab.com/sustainability">Sustainability</a></li> 74 <li class="nav-item"><a class="nav-link js-scroll-trigger" href="https://lbr-lab.com/#Our values">Our values</a></li> 75 <li class="nav-item"><a class="nav-link js-scroll-trigger" href="https://lbr-lab.com/#contact">Contact</a></li> 76 77 </li> 78 </ul> 79 </div> 80 </div> 81 </nav> 82 <!-- End Navigation --> 83 84 85 86<div class="container" id="pagecontainer"> 87<h2 id="available-3d-printable-files">Available 3D-printable files</h2> 88 89<div> 90 91 92 <div class="col-md col-sm-11 portfolio-item effect1"> 93 <a href="#r1" class="portfolio-link" data-toggle="modal"> 94 <img src="" class="img-fluid grid-img" alt="" /> 95 <div class="portfolio-caption"> 96 <h4>Print the 3D lobe divider (3DLD)</h4> 97 <p class="text-muted">The surgical guide used for the simoltaneous isolation of proximal and distal epithelial</p> 98 </div> 99 </a> 100 </div> 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117</div> 118 119<!-- project Modals --> 120 121<div class="portfolio-modal modal fade" id="r1" tabindex="-1" role="dialog" aria-hidden="true"> 122 123 <div class="modal-dialog"> 124 <div class="modal-content"> 125 <div class="close-modal" data-dismiss="modal"> 126 <div class="lr"> 127 <div class="rl"></div> 128 </div> 129 </div> 130 <div class="container"> 131 <div class="row"> 132 <div class="col-lg-8 mx-auto"> 133 <div class="modal-body"> 134 <h3 class="text-uppercase">Print the 3D lobe divider (3DLD)</h3> 135 136 <p><h3 id="simultaneous-isolation-of-proximal-and-distal-lung-progenitor-cells-from-individual-mice-using-a-3d-printed-guide-reduces-proximal-cell-contamination-of-distal-lung-epithelial-cell-isolations">Simultaneous isolation of proximal and distal lung progenitor cells from individual mice using a 3D printed guide reduces proximal cell contamination of distal lung epithelial cell isolations</h3> 137 138<p>
138The manuscript is now available on bioRxiv: https://www.biorxiv.org/content/10.1101/2022.05.10.491312v1</p> 139 140<p>You can download the stl files from the github repository: <a href="https://github.com/Lung-bioengineering-regeneration-lab/dual_cell_isolation/tree/main/3DLD_design">Click Here</a>.</p> 141 142<p><img src="assets/img/resources/3dld.png" width="60%;" /></p> 143 144</p> 145 146 <div> 147 <a class="btn btn-primary btn-xl text-uppercase js-scroll-trigger" href="https://www.biorxiv.org/content/10.1101/2022.05.10.491312v1">Go to published resource!</a> 148 <p> </p> 149 </div> 150 151 <button class="btn btn-primary" data-dismiss="modal" type="button"> 152 <i class="fas fa-times"></i> 153 "Close"</button> 154 </div> 155 </div> 156 </div> 157 </div> 158 </div> 159 </div> 160 </div> 161 162<div class="portfolio-modal modal fade" id="r2" tabindex="-1" role="dialog" aria-hidden="true"> 163 164 <div class="modal-dialog"> 165 <div class="modal-content"> 166 <div class="close-modal" data-dismiss="modal"> 167 <div class="lr"> 168 <div class="rl"></div> 169 </div> 170 </div> 171 <div class="container"> 172 <div class="row"> 173 <div class="col-lg-8 mx-auto"> 174 <div class="modal-body"> 175 <h3 class="text-uppercase">Decellularization of Human IPF lungs</h3> 176 177 <p><p>TGF-β1-induced deposition of provisional extracellular matrix by tracheal basal cells promotes epithelial-to-mesenchymal transition in a c-Jun NH2-terminal kinase-1-dependent manner</p> 178</p> 179 180 <div> 181 <a class="btn btn-primary btn-xl text-uppercase js-scroll-trigger" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032072/">Go to published resource!</a> 182 <p> </p> 183 </div> 184 185 <button class="btn btn-primary" data-dismiss="modal" type="button"> 186 <i class="fas fa-times"></i> 187 "Close"</button> 188 </div> 189 </div> 190 </div> 191 </div> 192 </div> 193 </div> 194 </div> 195 196<div class="portfolio-modal modal fade" id="r3" tabindex="-1" role="dialog" aria-hidden="true"> 197 198 <div class="modal-dialog"> 199 <div class="modal-content"> 200 <div class="close-modal" data-dismiss="modal"> 201 <div class="lr"> 202 <div class="rl"></div> 203 </div> 204 </div> 205 <div class="container"> 206 <div class="row"> 207 <div class="col-lg-8 mx-auto"> 208 <div class="modal-body"> 209 <h3 class="text-uppercase">Decellularization of Human (Normal, COPD) and Pig</h3> 210 211 <p><p>Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration</p> 212 213</p> 214 215 <div> 216 <a class="btn btn-primary btn-xl text-uppercase js-scroll-trigger" href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4215726/">Go to published resource!</a> 217 <p> </p> 218 </div> 219 220 <button class="btn btn-primary" data-dismiss="modal" type="button"> 221 <i class="fas fa-times"></i> 222 "Close"</button> 223 </div> 224 </div> 225 </div> 226 </div> 227 </div> 228 </div> 229 </div> 230 231<div class="portfolio-modal modal fade" id="r4" tabindex="-1" role="dialog" aria-hidden="true"> 232 233 <div class="modal-dialog"> 234 <div class="modal-content"> 235 <div class="close-modal" data-dismiss="modal"> 236 <div class="lr"> 237 <div class="rl"></div> 238 </div> 239 </div> 240 <div class="container"> 241 <div class="row"> 242 <div class="col-lg-8 mx-auto"> 243 <div class="modal-body"> 244 <h3 class="text-uppercase">Mouse lung decellularization</h3> 245 246 <p><p>The Preparation of Decellularized Mouse Lung Matrix Scaffolds for Analysis of Lung Regenerative Cell Potential</p> 247</p> 248 249 <div> 250 <a class="btn btn-primary btn-xl text-uppercase js-scroll-tr
250igger" href="https://link.springer.com/protocol/10.1007%2F978-1-4939-9086-3_20">Go to published resource!</a> 251 <p> </p> 252 </div> 253 254 <button class="btn btn-primary" data-dismiss="modal" type="button"> 255 <i class="fas fa-times"></i> 256 "Close"</button> 257 </div> 258 </div> 259 </div> 260 </div> 261 </div> 262 </div> 263 </div> 264 265<div class="portfolio-modal modal fade" id="r5" tabindex="-1" role="dialog" aria-hidden="true"> 266 267 <div class="modal-dialog"> 268 <div class="modal-content"> 269 <div class="close-modal" data-dismiss="modal"> 270 <div class="lr"> 271 <div class="rl"></div> 272 </div> 273 </div> 274 <div class="container"> 275 <div class="row"> 276 <div class="col-lg-8 mx-auto"> 277 <div class="modal-body"> 278 <h3 class="text-uppercase">Deconvolution of PCLS bulk RNASeq using IPF Atlas scRNAseq using Bisque</h3> 279 280 <p><p>You can find the code in the GitHub Repository: <a href="https://github.com/Lung-bioengineering-regeneration-lab/human_PCLS_BISQUE"> human PCLS BisqueRNA </a></p> 281 282<p>This code was produced for publication by first author John Stegmayr: 283<a href="https://journals.physiology.org/doi/abs/10.1152/ajplung.00401.2020"> Isolation of high yield and quality RNA from human precision-cut lung slices for RNA-sequencing and computational integration with larger patient cohorts </a></p> 284</p> 285 286 <div> 287 <a class="btn btn-primary btn-xl text-uppercase js-scroll-trigger" href="https://github.com/Lung-bioengineering-regeneration-lab/human_PCLS_BISQUE">Go to published resource!</a> 288 <p> </p> 289 </div> 290 291 <button class="btn btn-primary" data-dismiss="modal" type="button"> 292 <i class="fas fa-times"></i> 293 "Close"</button> 294 </div> 295 </div> 296 </div> 297 </div> 298 </div> 299 </div> 300 </div> 301 302<div class="portfolio-modal modal fade" id="r6" tabindex="-1" role="dialog" aria-hidden="true"> 303 304 <div class="modal-dialog"> 305 <div class="modal-content"> 306 <div class="close-modal" data-dismiss="modal"> 307 <div class="lr"> 308 <div class="rl"></div> 309 </div> 310 </div> 311 <div class="container"> 312 <div class="row"> 313 <div class="col-lg-8 mx-auto"> 314 <div class="modal-body"> 315 <h3 class="text-uppercase">New and improved RNA isolation protocol for PCLS</h3> 316 317 <p><p>Isolation of high yield and quality RNA from human precision-cut lung slices for RNA-sequencing and computational integration with larger patient cohorts</p> 318</p> 319 320 <div> 321 <a class="btn btn-primary btn-xl text-uppercase js-scroll-trigger" href="https://www.biorxiv.org/content/10.1101/2020.08.21.254516v1">Go to published resource!</a> 322 <p> </p> 323 </div> 324 325 <button class="btn btn-primary" data-dismiss="modal" type="button"> 326 <i class="fas fa-times"></i> 327 "Close"</button> 328 </div> 329 </div> 330 </div> 331 </div> 332 </div> 333 </div> 334 </div> 335 336<div class="portfolio-modal modal fade" id="r7" tabindex="-1" role="dialog" aria-hidden="true"> 337 338 <div class="modal-dialog"> 339 <div class="modal-content"> 340 <div class="close-modal" data-dismiss="modal"> 341 <div class="lr"> 342 <div class="rl"></div> 343 </div> 344 </div> 345 <div class="container"> 346 <div class="row"> 347 <div class="col-lg-8 mx-auto"> 348 <div class="modal-body"> 349 <h3 class="text-uppercase">PCLS generation from Human tissues</h3> 350 351 <p><p>Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling</p> 352</p> 353 354 <div> 355 <a class="btn btn-primary btn-xl text-uppercase js-scroll-tr
355igger" href="https://www.jove.com/t/58437/generation-human-3d-lung-tissue-cultures-3d-ltcs-for-disease">Go to published resource!</a> 356 <p> </p> 357 </div> 358 359 <button class="btn btn-primary" data-dismiss="modal" type="button"> 360 <i class="fas fa-times"></i> 361 "Close"</button> 362 </div> 363 </div> 364 </div> 365 </div> 366 </div> 367 </div> 368 </div> 369 370<div class="portfolio-modal modal fade" id="r8" tabindex="-1" role="dialog" aria-hidden="true"> 371 372 <div class="modal-dialog"> 373 <div class="modal-content"> 374 <div class="close-modal" data-dismiss="modal"> 375 <div class="lr"> 376 <div class="rl"></div> 377 </div> 378 </div> 379 <div class="container"> 380 <div class="row"> 381 <div class="col-lg-8 mx-auto"> 382 <div class="modal-body"> 383 <h3 class="text-uppercase">Name of resource</h3> 384 385 <p><p>Content of the new resource. Treat this as if it was a document. Format using markdown rules.</p> 386 387</p> 388 389 <div> 390 <a class="btn btn-primary btn-xl text-uppercase js-scroll-trigger" href="">Go to published resource!</a> 391 <p> </p> 392 </div> 393 394 <button class="btn btn-primary" data-dismiss="modal" type="button"> 395 <i class="fas fa-times"></i> 396 "Close"</button> 397 </div> 398 </div> 399 </div> 400 </div> 401 </div> 402 </div> 403 </div> 404 405<!-- End project Modals --> 406 407</div> 408 409 410 <!-- Footer --> 411 <footer class="footer" id="footer" style="background-color:white;"> 412 <div class="container"> 413 <div class="row align-items-center"> 414 <div class="col-md-4"> 415 <span class="copyright">Copyright © Lung Bioengineering and Regeneration Lab 2026</span> 416 </div> 417 <!-- Social Media --> 418 <div class="col-md-4"> 419 <ul class="list-inline social-buttons"> 420 421 <li class="list-inline-item"> 422 423 <a href="https://twitter.com/DWagnerLab/"> 424 <i class="fab fa-twitter"></i> 425 </a> 426 427 </li> 428 429 <li class="list-inline-item"> 430 431 <a href="https://github.com/lung-bioengineering-regeneration-lab/"> 432 <i class="fab fa-github"></i> 433 </a> 434 435 </li> 436 437 <li class="list-inline-item"> 438 439 <a href="https://www.instagram.com/dwagnerlab/"> 440 <i class="fab fa-instagram"></i> 441 </a> 442 443 </li> 444 445 </ul> 446 </div> 447 <!-- Legal --> 448 <div class="col-md-4"> 449 <ul class="list-inline quicklinks"> 450 <li class="list-inline-item"> 451 <a href="legal">Privacy Policy</a> 452 </li> 453 </ul> 454 </div> 455 </div> 456 </div> 457 </footer> 458
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