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39  <section data-bs-version="5.1" class="menu cid-sZGNcUTWCJ" once="menu" id="menu1-28">
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45                <span class="navbar-caption-wrap"><a class="navbar-caption text-success text-primary display-5" href="index.html">PT-OPENSCREEN</a></span>
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64</section>
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66<section data-bs-version="5.1" class="header1 cid-sWR506y31c" id="header1-y">
67
68    
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70    <div class="mbr-overlay" style="opacity: 0.6; background-color: rgb(255, 255, 255);"></div>
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72    <div class="align-center container">
73        <div class="row justify-content-center">
74            <div class="col-12 col-lg-9">
75                <h1 class="mbr-section-title mbr-fonts-style mb-3 display-2"><strong>Services</strong></h1>
76                
77                <p class="mbr-text mbr-fonts-style display-7">Working with different partners institutions, PT-OPENSCREEN offers a collection of services.&nbsp;<br>For service or information requests, please fill the <a href="contact.html" class="text-primary">contact form</a>&nbsp;and your request will be relayed to a suitable partner of the network.<br>
78<br>
79<br><br></p>
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82        </div>
83    </div>
84</section>
85
86<section data-bs-version="5.1" class="features1 cid-sFzyUE9AaP" id="features1-1i">
87    
88
89    
90    <div class="container-fluid">
91        <div class="row">
92            
93        </div>
94        <div class="row">
95            <div class="card col-12 col-md-6 col-lg-3">
96                <div class="card-wrapper">
97                    <div class="card-box align-center">
98                        <div class="iconfont-wrapper">
99                            <span class="mbr-iconfont mbrib-bold"></span>
100                        </div>
101                        <h5 class="card-title mbr-fonts-style display-5">Biology</h5>
102                        <p class="card-text mbr-fonts-style display-7">From standard assays to the development of new assays and analysis of the results</p>
103                    </div>
104                </div>
105            </div>
106            <div class="card col-12 col-md-6 col-lg-3">
107                <div class="card-wrapper">
108                    <div class="card-box align-center">
109                        <div class="iconfont-wrapper">
110                            <span class="mbr-iconfont socicon-quora socicon"></span>
111                        </div>
112                        <h5 class="card-title mbr-fonts-style display-5">Chemistry</h5>
113                        <p class="card-text mbr-fonts-style display-7">Compound bioprofiling of small molecule libraries on the panel of cell lines and data analysis.</p>
114                    </div>
115                </div>
116            </div>
117            <div class="card col-12 col-md-6 col-lg-3">
118                <div class="card-wrapper">
119                    <div class="card-box align-center">
120                        <div class="iconfont-wrapper">
121                            <span class="mbr-iconfont mobi-mbri-contact-form mobi-mbri"></span>
122                        </div>
123                        <h5 class="card-title mbr-fonts-style display-5"><a href="services.html#features8-1i" class="text-primary">Consultancy&nbsp;&amp; Analysis</a></h5>
124                        <p class="card-text mbr-fonts-style display-7">The management and execution of projects, identification of suitable experimental approach.</p>
125                    </div>
126                </div>
127            </div>
128            
129        </div>
130    </div>
131</section>
132
133<section data-bs-version="5.1" class="features7 cid-sX1KQuQUK4 mbr-parallax-background" id="features8-1e">
134    <!---->
135    
136    <div class="mbr-overlay" style="opacity: 0.5; background-color: rgb(255, 255, 255);">
137    </div>
138    <div class="container">
139        <div class="row">
140            <div class="title col-12">
141                
142                
143            </div>
144            <div class="card col-12 col-md-6">
145                <div class="card-wrapper">
146                    <div class="top-line">
147                        <h6 class="card-title mbr-fonts-style display-5"><strong>Assay miniaturization</strong></h6>
148                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
149                    </div>
150                    <div class="bottom-line">
151                        <p class="mbr-text mbr-fonts-style display-7">
152                            Adaptation of cell or biochemical assays to microwell plate format for screening to achieve robust and reproducible readouts with minimum intra and inter-plate variability.<br><br>CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S<br>
153                        </p>
154                    </div>
155                </div>
156            </div>
157            <div class="card col-12 col-md-6">
158                <div class="card-wrapper">
159                    <div class="top-line">
160                        <h6 class="card-title mbr-fonts-style display-5"><strong>Compound profiling</strong></h6>
161                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
162                    </div>
163                    <div class="bottom-line">
164                        <p class="mbr-text mbr-fonts-style display-7">
165                            Original small molecules or extracts from natural sources are profiled using different cellular models, including human cells from healthy and diseased-altered tissues. Assays to collect information about viability will suggest compounds with a promising therapeutic profile for follow-up experiments.<br><br>CBMA, CBA-UAC, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S<br>
166                        </p>
167                    </div>
168                </div>
169            </div>
170            <div class="card col-12 col-md-6">
171                <div class="card-wrapper">
172                    <div class="top-line">
173                        <h6 class="card-title mbr-fonts-style display-5"><strong>Pilot screening</strong></h6>
174                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
175                    </div>
176                    <div class="bottom-line">
177                        <p class="mbr-text mbr-fonts-style display-7">
178                            Screening of compound libraries of up to 5000 compounds as final job or as preparation to full screening campaigns of more than 100000 compounds. Includes hit confirmation and validation and may include follow-up studies.<br><br>CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S<br>
179                        </p>
180                    </div>
181                </div>
182            </div>
183            <div class="card col-12 col-md-6">
184                <div class="card-wrapper">
185                    <div class="top-line">
186                        <h6 class="card-title mbr-fonts-style display-5"><strong>High-Throughput screening</strong></h6>
187                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
188                    </div>
189                    <div class="bottom-line">
190                        <p class="mbr-text mbr-fonts-style display-7">
191                            Screening of compound libraries more than 100000 compounds. Includes hit confirmation and validation and may include follow-up studies.<br><br>i3S<br>
192                        </p>
193                    </div>
194                </div>
195            </div>
196            <div class="card col-12 col-md-6">
197                <div class="card-wrapper">
198                    <div class="top-line">
199                        <h6 class="card-title mbr-fonts-style display-5"><strong>Target deconvolution&nbsp;</strong></h6>
200                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
201                    </div>
202                    <div class="bottom-line">
203                        <p class="mbr-text mbr-fonts-style display-7">
204                            Cell and/or biochemical assays are designed and performed for target deconvolution of hits resulting from phenotypical-based screenings.<br><br>CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S<br></p>
205                    </div>
206                </div>
207            </div>
208            <div class="card col-12 col-md-6">
209                <div class="card-wrapper">
210                    <div class="top-line">
211                        <h6 class="card-title mbr-fonts-style display-5"><strong>Drug-target interactions</strong></h6>
212                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
213                    </div>
214                    <div class="bottom-line">
215                        <p class="mbr-text mbr-fonts-style display-7">Bio-Layer Interferometry is used to perform label-free, real-time study of biomolecular interactions (drug-receptor, protein-protein interactions) for determination of binding constants and kinetics.<br><br>CEB-UM<br></p>
216                    </div>
217                </div>
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226    </div>
227</section>
228
229<section data-bs-version="5.1" class="features7 cid-sX1Nvmqn7I mbr-parallax-background" id="features8-1g">
230    <!---->
231    
232    <div class="mbr-overlay" style="opacity: 0.5; background-color: rgb(255, 255, 255);">
233    </div>
234    <div class="container">
235        <div class="row">
236            <div class="title col-12">
237                
238                
239            </div>
240            <div class="card col-12 col-md-6">
241                <div class="card-wrapper">
242                    <div class="top-line">
243                        <h6 class="card-title mbr-fonts-style display-5"><strong>Evaluation of drug-like properties</strong></h6>
244                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
245                    </div>
246                    <div class="bottom-line">
247                        <p class="mbr-text mbr-fonts-style display-7">
248                            Evaluation of physicochemical properties (e.g. solubility, lipophilicity (LogD / LogP), pKa) that can affect biological assay results and cause incorrect structure-activity relationships. This evaluation is relevant in early drug discovery programs and necessary for ADMET studies.<br><br>CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE<br>
249                        </p>
250                    </div>
251                </div>
252            </div>
253            <div class="card col-12 col-md-6">
254                <div class="card-wrapper">
255                    <div class="top-line">
256                        <h6 class="card-title mbr-fonts-style display-5"><strong>Hit to lead or lead optimization</strong></h6>
257                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
258                    </div>
259                    <div class="bottom-line">
260                        <p class="mbr-text mbr-fonts-style display-7">
261                            For newly identified compounds with targeted biological activity (hits), optimization performed by medicinal chemistry tools is offered in order to obtain pharmacologically viable advanced candidates possessing optimized activity/potency/selectivity profiles and/or chemical novelty.<br><br>CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE<br>
262                        </p>
263                    </div>
264                </div>
265            </div>
266            <div class="card col-12 col-md-6">
267                <div class="card-wrapper">
268                    <div class="top-line">
269                        <h6 class="card-title mbr-fonts-style display-5"><strong>Custom synthesis</strong></h6>
270                        <p class="mbr-text cost mbr-fonts-style display-7"></p>
271                    </div>
272                    <div class="bottom-line">
273                        <p class="mbr-text mbr-fonts-style display-7">
274                            Synthesis of different chemical tools such as building blocks, standards for pharmacological and analytical purposes, degradation products or impurities, human metabolites or prodrugs to improve pharmacokinetic properties, in small to medium sized libraries.<br><br>CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE<br>
275                        </p>
276                    </div>
277                </div>
278            </div>
279            <div class="card col-12 col-md-6">
280                <div class="card-wrapper">
281                    <div class="top-line">
282                        <h6 class="card-title mbr-fonts-style display-5"><strong>Evaluation of drug or drug candidate stability</strong></h6>
283                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
284                    </div>
285                    <div class="bottom-line">
286                        <p class="mbr-text mbr-fonts-style display-7">The evaluation of compound storage stability (in solution or solid state) and compound metabolic 
286stability (namely in plasma and in hepatocytes) can be performed using chromatographic (UHPLC/HPLC) and spectrometric (NMR/MS) tools.<br>&nbsp;<br>CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, IMM, BioISI<br>
287                        </p>
288                    </div>
289                </div>
290            </div>
291            <div class="card col-12 col-md-6">
292                <div class="card-wrapper">
293                    <div class="top-line">
294                        <h6 class="card-title mbr-fonts-style display-5"><strong>Evaluation of drug or drug candidate permeability using biomimetic membrane models&nbsp;</strong></h6>
295                        <p class="mbr-text cost mbr-fonts-style display-7"></p>
296                    </div>
297                    <div class="bottom-line">
298                        <p class="mbr-text mbr-fonts-style display-7">
299                            BBB, gastro-intestinal and skin permeability prediction can be attained using non-cellular (Parallel Artificial Membrane Permeability Assay) permeability assays. If needed, permeability studies using cellular assays like Caco-2 cells, can be performed.<br><br>CIQUP, CQM, CQC, iMED.Ulisboa, CQE, IMM<br></p>
300                    </div>
301                </div>
302            </div>
303            <div class="card col-12 col-md-6">
304                <div class="card-wrapper">
305                    <div class="top-line">
306                        <h6 class="card-title mbr-fonts-style display-5"><strong>Quality control of compounds</strong></h6>
307                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
308                    </div>
309                    <div class="bottom-line">
310                        <p class="mbr-text mbr-fonts-style display-7">The consortium has the necessary equipment to perform quality control of libraries of compounds and isolation/ purification of samples. The consortium also has equipment for chemical characterization (NMR, HPLC-MS).<br><br>CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, BioISI<br></p>
311                    </div>
312                </div>
313            </div>
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320        </div>
321    </div>
322</section>
323
324<section data-bs-version="5.1" class="features7 cid-sX1SrjFkPq mbr-parallax-background" id="features8-1h">
325    <!---->
326    
327    <div class="mbr-overlay" style="opacity: 0.5; background-color: rgb(255, 255, 255);">
328    </div>
329    <div class="container">
330        <div class="row">
331            <div class="title col-12">
332                
333                
334            </div>
335            <div class="card col-12 col-md-6">
336                <div class="card-wrapper">
337                    <div class="top-line">
338                        <h6 class="card-title mbr-fonts-style display-5"><strong>Construction of chemical libraries</strong></h6>
339                        <p class="mbr-text cost mbr-fonts-style display-7"></p>
340                    </div>
341                    <div class="bottom-line">
342                        <p class="mbr-text mbr-fonts-style display-7">
343                            Synthesis of small focused libraries for screening based on target or compound class, isolation of novel compounds from biological extracts (different chromatographic techniques) and characterization of new structures (NMR, HPLC-MS).<br><br>CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, CIIMAR<br>
344                        </p>
345                    </div>
346                </div>
347            </div>
348            <div class="card col-12 col-md-6">
349                <div class="card-wrapper">
350                    <div class="top-line">
351                        <h6 class="card-title mbr-fonts-style display-5"><strong>Access to compound libraries</strong></h6>
352                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
353                    </div>
354                    <div class="bottom-line">
355                        <p class="mbr-text mbr-fonts-style display-7">
356                            Chemical banking for management and storage will provide access to libraries of bioactive, target-focused or diversity-based compounds, fragments, biological extracts, to compounds produced by Portuguese chemists and, as part of the EU-OPENSCREEN, to the European Chemical Biology Library.<br><br>i3S, CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM<br>
357                        </p>
358                    </div>
359                </div>
360            </div>
361            <div class="card col-12 col-md-6">
362                <div class="card-wrapper">
363                    <div class="top-line">
364                        <h6 class="card-title mbr-fonts-style display-5"><strong>Access to libraries of tools for genome-wide gene perturbation</strong></h6>
365                        <p class="mbr-text cost mbr-fonts-style display-7"></p>
366                    </div>
367                    <div class="bottom-line">
368                        <p class="mbr-text mbr-fonts-style display-7">
369                            Libraries of siRNAs, microRNAs, dsRNAs (C.elegans), CRISPR (guide RNA) and yeast mutants allow the identification of disease-relevant target genes that could be further used as basis for the development of target-based chemical screens.<br><br>CNC, ICVS, BioISI, CBMA, i3S<br>
370                        </p>
371                    </div>
372                </div>
373            </div>
374            <div class="card col-12 col-md-6">
375                <div class="card-wrapper">
376                    <div class="top-line">
377                        <h6 class="card-title mbr-fonts-style display-5"><strong>Access to readout instruments</strong></h6>
378                        <p class="mbr-text cost mbr-fonts-style display-7"></p>
379                    </div>
380                    <div class="bottom-line">
381                        <p class="mbr-text mbr-fonts-style display-7">
382                            Access to microplate readers (absorbance, fluorescence, luminescence and related technologies) and automated widefield and confocal microscopes (high-content assays).<br><br>CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S<br>
383                        </p>
384                    </div>
385                </div>
386            </div>
387            <div class="card col-12 col-md-6">
388                <div class="card-wrapper">
389                    <div class="top-line">
390                        <h6 class="card-title mbr-fonts-style display-5"><strong>Medicinal Chemistry projects and consulting</strong></h6>
391                        <p class="mbr-text cost mbr-fonts-style display-7"></p>
392                    </div>
393                    <div class="bottom-line">
394                        <p class="mbr-text mbr-fonts-style display-7">
395                            The consortium has experience in the management and execution of projects of preclinical Medicinal Chemistry. The consortium offers both consultancy in project management and design, synthesis of dedicated libraries and characterization of new structures (NMR, HPLC-MS).<br><br>CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, BioISI<br>
396                        </p>
397                    </div>
398                </div>
399            </div>
400            <div class="card col-12 col-md-6">
401                <div class="card-wrapper">
402                    <div class="top-line">
403                        <h6 class="card-title mbr-fonts-style display-5"><strong>Consultancy on experimental screening strategy</strong></h6>
404                        <p class="mbr-text cost mbr-fonts-style display-7"></p>
405                    </div>
406                    <div class="bottom-line">
407                        <p class="mbr-text mbr-fonts-style display-7">
408                            Identification of suitable experimental approach: cell or biochemical assay, chemical libraries size and composition, selection of suitable material and reagents, readout technology such as absorbance, fluorescence intensity, luminescence, high-content screening on automated fluorescence microscope.<br><br>CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CIQUP, CNC, CQM, CQUM, CQC, CQE, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S<br>
409                        </p>
410                    </div>
411                </div>
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419        </div>
420    </div>
421</section>
422
423<section data-bs-version="5.1" class="features7 cid-sX1UhaSt5X" id="features8-1i">
424    <!---->
425    
426    
427    <div class="container">
428        <div class="row">
429            <div class="title col-12">
430                
431                
432            </div>
433            <div class="card col-12 col-md-6">
434                <div class="card-wrapper">
435                    <div class="top-line">
436                        <h6 class="card-title mbr-fonts-style display-5"><strong>Bioimage analysis</strong></h6>
437                        <p class="mbr-text cost mbr-fonts-style display-5"></p>
438                    </div>
439                    <div class="bottom-line">
440                        <p class="mbr-text mbr-fonts-style display-7">
441                            Automated processing of microscopy images (fluorescence or transmission) using appropriate software tools and IT infrastru
441cture to extract quantitative phenotypic data in numerical form. Extraction of multi-parametric quantitative features at the single cell level for high content screening assays.<br><br>CEDOC, CNC, BioISI, ICVS, iMM, i3S, iBiMED<br>
442                        </p>
443                    </div>
444                </div>
445            </div>
446            <div class="card col-12 col-md-6">
447                <div class="card-wrapper">
448                    <div class="top-line">
449                        <h6 class="card-title mbr-fonts-style display-5"><strong>Statistical data analysis</strong></h6>
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454                            Efficient analysis of large numerical datasets produced by spectroscopy or image-based screening using data science tools to report the phenotypic variation caused by each chemical perturbation. Will include data visualization, exploratory data analysis, quality control and normalization.<br><br>CNC, ICVS, BioISI, iMM, i3S<br>
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