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310w, 1920px" /></div></li></ul></div></div> 311<div id='av_section_1' class='avia-section av-7e2pak-bc26f135c64db155f43cfb27f2e26049 main_color avia-section-default avia-no-border-styling avia-builder-el-1 el_after_av_slideshow_full el_before_av_section avia-bg-style-scroll container_wrap fullsize' ><div class='container av-section-cont-open' ><div class='template-page content av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-1154'><div class='entry-content-wrapper clearfix'> 312<div class='hr av-jilpbq1p-ccb548b6cac2a9ace05d5fbbbe5fd940 hr-invisible avia-builder-el-2 el_before_av_one_full avia-builder-el-first '><span class='hr-inner '><span class="hr-inner-style"></span></span></div> 313<div class='flex_column av-5ggwyk-fd8550f56ed8bc6372c2ec52e43a9490 av_one_full avia-builder-el-3 el_after_av_hr avia-builder-el-last first flex_column_div av-animated-generic bottom-to-top av-zero-column-padding ' ><section class='av_textblock_section av-4z7ruk-d2487011fda81eea7c9d7ddcfc7167a7 ' itemscope="itemscope" itemtype="https://schema.org/CreativeWork" ><div class='avia_textblock' itemprop="text" ><h2><span style="color: #333399;">Si-Click<sup>®</sup> â (Copper-free) Click-Chemistry for Bioorthogonal Labeling</span></h2> 314<p>Click chemistry is a class of chemical reactions between 2 reactive compounds that occur with <strong>high efficiency and specificity</strong>, without the need for special solvents or even catalysts. SiChem offers a comprehensive range of <strong>click chemistry reagents</strong> for bioorthogonal applications in cell biology and drug development.</p> 315<p>This characteristic can be used very well for labeling proteins in intact cells: Mounting labels on proteins allows observing and tracking them in their natural environment and represent a powerful tool for studying cell biology and even for developing drugs. Nowadays, a large variety of approaches exists for protein labeling â differing in size of modification, general scope, as well as the ease of usability and exchangeability.</p> 316<p>A major drawback of many genetically encoded tags represents their large size which can be overcome by making use of the<strong> fascinating potential of specifically labeling single amino acid residues with small molecule dyes.</strong> Genetically encodable unnatural amino acids (UAAs) allowing for biocompatible and even bioorthogonal site-specific labeling of proteins with (fluorogenic) dyes are under development.</p> 317<p>The new lysine-based compounds have (bi)cyclooctyne (e.g. <a href="https://shop.sichem.de/en/click-chemistry/sc-8000.html"><strong>SCO</strong></a><strong> / <a href="https://shop.sichem.de/en/click-chemistry/sc-8014.html">BCN</a></strong>), trans-cyclooctene (e.g. <strong><a href="https://shop.sichem.de/en/click-chemistry/sc-8004.html">TCO-4-en</a> / <a href="https://shop.sichem.de/en/click-chemistry/sc-8008.html">TCO*</a></strong>) or propargyl units (e.g. <a href="https://shop.sichem.de/en/click-chemistry/sc-8002.html"><strong>PrK</strong></a>) that can be grafted into proteins â amongst other of E. coli and mammalian cells. <strong>Copper(I)-catalyzed Azide-Alkyne Cycloaddition (CuAAC)</strong> click chemistry between azides and linear alkynes as well as the two most potent representatives of in vivo bioorthogonal chemical reactions, <strong>strain-promoted alkyne-azide cycloaddition (SPAAC)</strong> and<strong> strain-promoted inverse electron-demand Diels-Alder cycloaddition (SPIEDAC)</strong>, can be used to efficiently and rapidly attach fluorophores conjugated to azide and tetrazine moieties, respectively. Due to the modularity of the technique, any imaginable probe fused to azide or tetrazine can be attached to any protein modified with a corresponding unnatural side chain with the maximum freedom of placement within the protein under investigation.</p> 318<h2><span style="color: #333399;">Click Chemistry Reagents for ADC & SPR Applications</span></h2> 319<p>The strained rings (cyclooctin, transcyclooctene) are also used for the triggered release of APIs: The click reaction and thus the selected cleavage of tumor-bound <strong>antibody drug conjugates (ADCs)</strong> can be a powerful tool in ADC therapy.</p> 320<p>Another application of the click reagents is in <strong>surface plasmon resonance (SPR)</strong> – a widely used method for <strong>characterizing bindings</strong>, e.g. between small molecules and target proteins. Here, the sensors and the ligands (e.g. proteins) are tagged with appropriate click tools and can thus interact quantitatively and extremely selectively.</p> 321<p>Relevant tools you will find here: <strong><a href="https://www.sichem.de/wp-content/uploads/2019/03/SiChem_PEG-tools.pdf" target="_blank" rel="noopener">SiChem_PEG-tools</a></strong> or <a href="https://shop.sichem.de/en/si-pegs.html">i<strong>n our Shop</strong></a></p> 322<h2><span style="color: #333399;"><strong>Custom Click Chemistry Reagents at Scale</strong></span></h2> 323<p>SiChem develops customized click amino acids with different substituents and (PEG) linkers or dyes according to your specifications. Quantities in kg scale can be produced in high purity in our fully equipped laboratories.</p> 324<p>Also corresponding precursors, such as various <span style="color: #333399;"><strong>active esters and alcohols</strong></span> with strained ring-systems can be synthesized in larger quantities.</p> 325</div></section></div></div></div></div><!-- close content main div --></div></div><div id='av_section_2' class='avia-section av-7e2pak-7-5e213156a8c0421a7d4dbef7d498e615 main_color avia-section-default avia-no-border-styling avia-builder-el-5 el_after_av_section el_before_av_icon_box avia-bg-style-scroll container_wrap fullsize' ><div class='container av-section-cont-open' ><div class='template-page content av-content-full alpha units'><div class='post-entry post-entry-type-page post-entry-1154'><div class='entry-content-wrapper clearfix'> 326<div class='flex_column av-5ggwyk-5-96ba2932c96f45a95fcaf5e804c802aa av_one_third avia-builder-el-6 el_before_av_two_fifth avia-builder-el-first first flex_column_div av-animated-generic bottom-to-top ' ><section class='av_textblock_section av-4z7ruk-4-05d1555764d53db12eaad1976fc5d73e ' itemscope="itemscope" itemtype="https://schema.org/CreativeWork" ><div class='avia_textblock' itemprop="text" ><p><strong>Copper(I)-catalyzed </strong></p> 327</div></section></div><div class='flex_column av-5ggwyk-3-6aa5d101ba38d34143a41ea5ea1009d8 av_two_fifth avia-builder-el-8 el_after_av_one_third el_before_av_one_full flex_column_div av-animated-generic bottom-to-top ' ><section class='av_textblock_section av-4z7ruk-2-23147278c0bcfb825ebd56cb809dbe8e ' itemscope="itemscope" itemtype="https://schema.org/CreativeWork" ><div class='avia_textblock' itemprop="text" ><p><strong>Copper-free </strong></p> 328</div></section></div><div class='flex_column av-5ggwyk-1-7da5c7702b25e772a7c6ca61657144a4 av_one_full avia-builder-el-10 el_after_av_two_fifth avia-builder-el-last first flex_column_div av-animated-generic bottom-to-top ' ><div class='avia-image-container av-jilro5vt-020a5b9c1a7e2693d058afb7e1c22170 av-styling- avia-align-center avia-builder-el-11 avia-builder-el-no-sibling ' itemprop="image" itemscope="itemscope" itemtype="https://schema.org/ImageObject" ><div class="avia-image-container-inner"><div class="avia-image-overlay-wrap"><img decoding="async" fetchpriority="high" class='wp-image-2304 avia-img-lazy-loading-not-2304 avia_image ' src="https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-scaled.jpg" alt='reaction speed in Click-Chemistry' title='Si-Click_05_xl' height="698" width="2560" itemprop="thumbnailUrl" srcset="https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-scaled.jpg 2560w, https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-300x82.jpg 300w, https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-1030x281.jpg 1030w, https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-768x209.jpg 768w, https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-1536x419.jpg 1536w, https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-2048x559.jpg 2048w, https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-1500x409.jpg 1500w, https://www.sichem.de/wp-content/uploads/2021/03/Si-Click_05_xl-705x192.jpg 705w" sizes="(max-width: 2560px) 100v
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