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111<body> 112<!--header--> 113<header id="stuck_container"> 114 <div class="container"> 115 <h1 class="navbar-brand navbar-brand_"><a href="index.php" title="LifeB | Laboratory of interaction and function of essential biomolecules"><img src="img/logo.png" alt="LifeB | Laboratory of interaction and function of essential biomolecules" title="LifeB | Laboratory of interaction and function of essential biomolecules"></a></h1> 116 <nav class="navbar navbar-default navbar-static-top tm_navbar clearfix" role="navigation"> 117 <strong><ul class="nav sf-menu clearfix"> 118 <li class="sub-menu"><a href="index.php" class="home " title="Home">Home<i></i></a> 119 <li><a href="research.php" class="research active" title="Research">Research<i></i></a></li> 120 <li><a href="publications.php" class="publications " title="Publications">Publications<i></i></a></li> 121 <li><a href="our-team.php" class="team " title="Lab Team">Lab Team<i></i></a></li> 122 <li><a href="graduates.php" class="graduates " title="Graduates">Graduates<i></i></a></li> 123 <li><a href="laboratory.php" class="lab " title="Laboratory">Laboratory<i></i></a></li> 124 <li><a href="contact.php" class="contact " title="Contact us">Contact us<i></i></a></li> 125 </li> 126 </ul> 127</nav> <select class="select-menu"> 128 <option value="#">Navigate to... 129 </option> 130 <option value="index.php">Home 131 </option> 132 <option value="research.php">Research 133 </option> 134 <option value="publications.php">Publications 135 </option> 136 <option value="our-team.php">Lab Team 137 </option> 138 <option value="graduates.php">Graduates 139 </option> 140 <option value="laboratory.php">Laboratory 141 </option> 142 <option value="contact.php">Contact us 143 </option> 144 </select> 145 </div> 146</header><div class="content indent"> 147 <!--content--> 148 <div class="thumb-box4" style="padding-top:0px; background:transparent;"> 149 <div class="container"> 150 <div class="row"> 151 <div class="col-lg-12" style="text-align:center;"> 152 <p class="title wow fadeIn animated" style="visibility: visible; animation-name: fadeIn;">Research</p> 153 </div> 154 </div> 155 </div> 156 </div> 157 158 <div class="thumb-box10"> 159 <div class="container"> 160 <div class="row"> 161 </div> 162 <div class="col-lg-4 col-md-4 col-sm-12 col-xm-12"> 163 <ul style="padding-left:20px;"> 164<li style="color:#658c07; margin-bottom:15px;"><h3 style="margin-bottom:0px;"><strong>We measure</strong></h3> 165<p style="display:inline;color:#658c07;">how and why life essential proteins and nucleic acids interact</p></li> 166 167<li style="color:#658c07; margin-bottom:15px;"> 168<h3 style="margin-bottom:0px;"><strong>We quantify</strong></h3> 169<p style="display:inline;color:#658c07;">affinity and kinetics of nucleoprotein complexes formation using quantitative biochemical and biophysical approaches </p></li> 170 171<li style="color:#658c07; margin-bottom:15px;"><h3 style="margin-bottom:0px;"><strong>We analyze</strong></h3> 172<p style="display:inline;color:#658c07;">functions of biomolecules <em>in vitro</em> and in live cells by advanced spectroscopy and microscopy techniques</p></li> 173 174<li style="color:#658c07; margin-bottom:15px;"><h3 style="margin-bottom:0px;"><strong>We apply</strong></h3> 175<p style="display:inline;color:#658c07;"> our quantitative results to reveal mechanisms how biomolecules contribute to genome stability, cell regeneration, aging and carcinogenesis</p></li> 176</ul> 177 </div> 178 <div class="col-lg-4 col-md-4 col-sm-12 col-xm-12"> 179 <div class="thumb-pad1 wow fadeInUp"> 180 <h2 style="font-size:30px;">Inherited aneamia CDA-I: how protein mutations carve âSwissâcheeseâ chromatin </h2> 181 <div class="thumbnail"> 182 <figure><a href="img/research_CDA-I.jpg" class="fancybox"><img src="img/research_CDA-I.png" alt="" title="Inhereted anemia CDA-I"></a></figure> 183 <div class="caption"> 184 <p>Congenital dyserythropoietic anemia type I (CDAâI) is associated with mutations in Codanin1 and CDIN1. We investigate the molecular mechanism linking mutate protein variants to defective erythroblast spongy chromatin that remind "Swiss cheese" under microscope. We combine biophysical, structural, and cellular approach to define how CDAâI mutations disrupt protein partner competition, impair nuclear histone supply, and reshape chromatin architecture. Using quantitative binding analysis, exch
184ange kinetics, and microscopic structural mapping, we reveal how selected mutations alter chromatin and erythroblast function. We employ advanced cellular models analyzed by highâresolution electron microscopy, and genomeâwide accessibility profiling to reveal mutationâinduced chromatin alterations. 185 Together, we define a unified mechanistic model of CDAâI pathogenesis and identify molecular markers relevant for clinical variant interpretation. </p> 186 <!--<a href="#" class="btn-default btn2">Stáhnout publikaci</a>--> 187 </div> 188 </div> 189 </div> 190 </div> 191 192 <div class="col-lg-4 col-md-4 col-sm-12 col-xm-12"> 193 <div class="thumb-pad1 wow fadeInUp"> 194 <h2 style="font-size:30px;">EBV kissing virus - hijacker of human cells: transcription factor Rta as viral switch</h2> 195 <div class="thumbnail"> 196 <figure><a href="img/research_EBV.png" class="fancybox"><img src="img/research_EBV.png" alt="" title="Rta effects upon EBV infection"></a></figure> 197 <div class="caption"> 198 <p>EpsteinâBarr virus (EBV) infects more than 90% of people worldwide, is commonly transmitted via saliva which earned it the nickname âkissingâ virus, and can produce in infected tissues as up to 100,000 billion viral particles. EBV targets B lymphocytes and epithelial cells, drives cancers that depend on the infected cell type, and is linked to mononucleosis and autoimmune conditions including a strong epidemiological association with multiple sclerosis. We map how the lytic activator Rta recognizes DNA, multimerizes, and concentrates in nuclear foci to remodel chromatin and trigger focal hostâchromosome disruption. Combining quantitative fluorescence, targeted mutagenesis, bioinformatic modeling, AFM, and genomeâwide chromatin profiling, we identify the residues and domains that mediate sequenceâspecific contacts and cooperative Rta assembly on DNA, reveal Rtaâs role as a critical switch from latency to lytic replication, and expose molecular targets for interventions to block EBV reactivation and its pathogenic consequences. </p> 199 <!--<a href="#" class="btn-default btn2">Stáhnout publikaci</a>--> 200 </div> 201 </div> 202 </div> 203 </div> 204 205 206 <div class="col-lg-4 col-md-4 col-sm-12 col-xm-12"> 207 <div class="thumb-pad1 wow fadeInUp"> 208 <h2 style="font-size:30px;">Dynamics of telomere maintenance</h2> 209 <div class="thumbnail"> 210 <figure><a href="img/reseach_telomere.png" class="fancybox"><img src="img/reseach_telomere.png" alt="" title="Dynamics of telomere maintenance"></a></figure> 211 <div class="caption"> 212 <p>Telomeres are complexes of DNA and proteins that are located at chromosome ends. The main function of telomeres is to protect genetic information stored in internal parts of chromosomes. Telomeres serves as molecular clock of cell life. Telomeres shorten during each cell division. If telomeres shorten below a certain length, cells die. Telomeric DNA covered by telomeric protein might form lasso-like loops that hide very ends of DNA and protect chromosome ends against their unwanted recognition as damaged DNA. We want to know how human telomeric proteins affect DNA and other proteins in order to understand molecular mechanism of the protective function of telomeres. </p> 213 <!--<a href="#" class="btn-default btn2">Stáhnout publikaci</a>--> 214 </div> 215 </div> 216 </div> 217 </div> 218 219 <div class="col-lg-4 col-md-4 col-sm-12 col-xm-12"> 220 <div class="thumb-pad1 wow fadeInUp"> 221 <h2 style="font-size:30px;">Controling telomerase activity</h2> 222 <div class="thumbnail"> 223 <figure><a href="img/shelterin_telomerase.png" class="fancybox"><img src="img/shelterin_telomerase.png" alt="" title="Controling telomerase activity"></a></figure> 224 <div class="caption"> 225 <p>Telomerase is an enzyme that extends telomeric DNA. Telomerase is idle in somatic cells but it is highly active in cancer and germ cells. Thus, telomerase contribute to âimmortalizationâ of frequently dividing germ cells and unfortunately also cancer
225cells. Telomerase action is affected by telomeric proteins. We want to understand mechanism how to turn off telomerase activity in cancer cells and this way defeat cancer more effectively. </p> 226 <!--<a href="#" class="btn-default btn2">Stáhnout publikaci</a>--> 227 </div> 228 </div> 229 </div> 230 </div> 231 232 <div class="clr"></div> 233 <div class="col-lg-4 col-md-4 col-sm-12 col-xm-12"> 234 235 </div> 236 <div class="col-lg-8 col-md-8 col-sm-12 col-xm-12" style="text-align:center;"> 237 <p>If you think that OUR experience, know-how and instrumentation is compatible with YOUR ideas and research topics, we can collaborate on an exciting project. </p> 238 <a href="contact.php" class="btn-default btn2" style="margin:auto;">Contact us</a> 239 </div> 240 241 242 243 244 <!--<div class="col-lg-4"> 245 <div class="wow fadeInUp"> 246 <h2>Categories<span>Cras Pulvina</span></h2> 247 <ul class="list1-1 indent wow fadeInUp"> 248 <li><a href="#">Nullam sed velit quam</a></li> 249 <li><a href="#">Pellentesque at neque vulpu</a></li> 250 <li><a href="#">Ate, suscipit enim eu</a></li> 251 <li><a href="#">Suscipit ante</a></li> 252 <li><a href="#">Pellentesque habitant morbi</a></li> 253 <li><a href="#">Senectus et netus et males</a></li> 254 <li><a href="#">Fames ac turpis eges</a></li> 255 <li><a href="#">Ultricies pretium</a></li> 256 <li><a href="#">Ut nulla magna, tempor non</a></li> 257 </ul> 258 </div> 259 <div class="wow fadeInUp" data-wow-delay="0.1s"> 260 <h2>Archives<span>Quisque</span></h2> 261 <ul class="list1-1"> 262 <li><a href="#">June 2013</a></li> 263 <li><a href="#">May 2013</a></li> 264 <li><a href="#">April 2013</a></li> 265 <li><a href="#">March 2013</a></li> 266 <li><a href="#">February 2013</a></li> 267 <li><a href="#">January 2013</a></li> 268 <li><a href="#">December 2012</a></li> 269 <li><a href="#">November 2012</a></li> 270 <li><a href="#">October 2012</a></li> 271 </ul> 272 </div> 273 </div>--> 274 </div> 275 </div> 276 </div> 277</div> 278 279 280<div class="contactFooter"> 281 <div class="container"> 282 <div class="row"> 283 284 285 286 287 288 <div class="col-sm-5"> 289 <div class="contacts"> 290 <article> 291 <img src="img/logo02.png" alt="LifeB" title="LifeB" class="logo02"> 292 <table> 293 <tr> 294 <td>+420 549 495 952</td> 295 </tr> 296 <tr> 297 <td><a href="mailto:[email protected]">[email protected]</a></td> 298 </tr> 299 </table> 300 <table> 301 <tr> 302 <td>Building C02/2.13<br> 303 Kamenice 753/5<br> 304 625 00 Bohunice - Brno</td> 305 </tr> 306 </table> 307 </article> 308 </div> 309 </div> 310 311 <!-- Replaced Mapy.cz iframe with centered coordinates --> 312<style> 313 .mapycz-map { position: relative; overflow: hidden; } 314 .mapycz-map iframe { display:block; transform: translateY(72px); } 315</style> 316 317<div class="col-sm-7"> 318 <section class="content_map"> 319 <div class="mapycz-map"> 320 <iframe 321 style="border:none" 322 src="https://mapy.com/s/horobuvuma" 323 width="150%" 324 height="380" 325 frameborder="0" 326 loading="lazy" 327 title="Map showing LifeB Laboratory location"> 328 </iframe> 329 </div> 330 </section> 331</div> 332 333 <!-- END Replaced Mapy.cz iframe with centered coordinates --> 334 </div> 335 </div> 336 </div> 337 338
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