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68					<li><a href="javascript:void(0);" data-pos="header">TOP</a></li>
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87    			<div class="hidden-sp">
88                    <h1>MHC Tetramers</h1>
89                    <p>Useful Tools for Immune Monitoring</p>
90                </div>
91    			<div class="hidden-pc">
92                    <h1><img src="images/main-sp.jpg" alt="MBL产品MHC Tetramer"></h1>
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118		</div>
119
120
121		<div class="wechat wechat-1 view_in_target_01" id="scroll_lp_tetramer_cn_A">
122    		<div class="title">As a partner in your drug discovery and cancer immunotherapy</div>
123			<div class="row">
124    			<div class="col"><a class="btn-pdf" href="./tetramer_EN.pdf" target="_blank"><span>Brochures</span></a></div>
125    			<div class="col"><a class="btn-qq js-qq" href="https://ruo.mbl.co.jp/bio/g/inq/" target="_blank" id="qqBtn_cta01"><span>Contact us</span></a></div>
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144			</div>
145			
146		</div>
147		
148		<div class="section section-1" id="special">
149			<h2 class="title">Our MHC Tetramers enable rapid and simple detection<br>of antigen-specific T cells of your interest.<div class="line"></div></h2>
150            <div class="sub-title">To date, antigen-specific T cells have been examined indirectly by detection of cytokines or cytotoxic activities. Now, MHC Tetramers make it possible for direct detection and specific isolation of antigen-specific T cells. You can determine functions and phenotypes of the target T cells using the MHC tetramer technology.</div>
151			<div class="box">
152				<img src="images/section_1_2.jpg" alt="">
153			</div>
154		</div>
155
156		<div class="wechat wechat-2 view_in_target_02" id="scroll_lp_tetramer_cn_B">
157    		<div class="title">As a partner in your drug discovery and cancer immunotherapy</div>
158			<div class="row">
159    			<div class="col"><a class="btn-pdf" href="./tetramer_EN.pdf" target="_blank"><span>Brochures</span></a></div>
160    			<div class="col"><a class="btn-qq js-qq" href="https://ruo.mbl.co.jp/bio/g/inq/" target="_blank" id="qqBtn_cta02"><span>Contact us</span></a></div>
161			</div>
162		</div>
163
164		<div class="section section-2" id="compare">
165			<h2 class="title">Advantages over Competitors<div class="line"></div></h2>
166			<div class="sub-title">Our MHC Tetramer shows a higher performance in specificity and target separation</div>
167			<div class="box box-shadow">
168				<div class="left">
169					<img class="pc-414" src="images/section_2_1.png" alt="">
170					<img class="mb-414" src="images/mb_section_2_1.png" alt="">
171				</div>
172				<div class="right">
173					<img class="pc-414" src="images/section_2_2.png" alt="">
174					<img class="mb-414" src="images/mb_section_2_2.png" alt="">
175				</div>
176			</div>
177		</div>
178
179		<div class="section section-3">
180			<h2 class="title">Comparison with Competitors(MHC Class I)<div class="line"></div></h2>
181			<div class="box">
182				<table class="pc" border="0" cellspacing="0" cellpadding="0">
183					<tbody>
184						<tr class="header">
185							<th class="col-1"></th>
186							<th class="center"><b>MBL MHC Class I Tetramer</b></th>
187							<th><b>Competitor’s Multimer</b></th>
188						</tr>
189						<tr>
190							<td class="col-1">Reactivity</td>
191							<td class="center anchor" data-pos="advantage" id="click_text-link_reactivity">Our MHC tetramers show high specificity due to a patented mutation (alanine to valine at position 245) in the MHC class I heavy chain, which reduces undesirable CD8-MHC interaction.</td>
192							<td>No comments regarding reactivity of competitor’s multimers.</td>
193						</tr>
194						<tr>
195							<td class="col-1">Quality</td>
196							<td class="center anchor" data-pos="compare" id="click_text-link_sensitivity">Optimized mixing ratio of raw materials dramatically improves tetramer synthesis efficiency and achieves high sensitivity.</td>
197							<td>Competitor’s multimers sometimes show low reproducibility due to difficulties in quality and quantity control of the number of MHC molecules when producing multimers. </td>
198						</tr>
199						<tr>
200							<td class="col-1">Stability</td>
201							<td class="center">Can be stored at 4°C in refrigerated form for several months, and is ready-to-use.</td>
202							<td>Storage stability is equivalent to MBL MHC tetramer.</td>
203						</tr>
204						<tr>
205							<td class="col-1">Custom MHC Tetramer</td>
206							<td class="center">Availability of more than 60 types of MHC alleles.</td>
207							<td>Only 20-30 types of alleles.</td>
208						</tr>
209					</tbody>
210				</table>
211
212				<img class="mb-768" src="images/mb_table_1.png" alt="">
213			</div>
214		</div>
215
216		<div class="wechat wechat-2 view_in_target_03" id="scroll_lp_tetramer_cn_C">
217    		<div class="title">As a partner in your drug discovery and cancer immunotherapy</div>
218			<div class="row">
219    			<div class="col"><a class="btn-pdf" href="./tetramer_EN.pdf" target="_blank"><span>Brochures</span></a></div>
220    			<div class="col"><a class="btn-qq js-qq" href="https://ruo.mbl.co.jp/bio/g/inq/" target="_blank" id="qqBtn_cta03"><span>Contact us</span></a></div>
221			</div>
222		</div>
223
224		<div class="section section-4" id="news">
225			<h2 class="title">MHC tetramer is used worldwide.<div class="line"></div></h2>
226			<div class="sub-title">World map of publications <br class="mb-414" />using MHC Tetramer</div>
227			<div class="box">
228				<img class="pc-414" src="images/section_4.png" alt="">
229				<img class="mb-414" src="images/mb_section_4.png" alt="">
230			</div>
231			<div class="section-footer"><a href="./referencelist-201809.pdf" target="_blank" class="btn-pdf"><span>Citation list</span></a></div>
232		</div>
233		
234		
235		<div class="section section-5">
236			<h2 class="title">Process of MHC Class I Tetramer Synthesis<div class="line"></div></h2>
237			<div class="box box-shadow">
238				<img class="pc-768" src="images/section_5.png" alt="">
239				<img class="mb-768" src="images/mb_section_5_1.png" alt="">
240				<img class="mb-768" src="images/mb_section_5_2.png" alt="">
241			</div>
242		</div>
243
244		<div class="section section-6">
245			<h2 class="title">Detection of Antigen-Specific T Cells by MHC Tetramers<div class="line"></div></h2>
246			<div class="box box-shadow">
247				<div class="left">
248					<!-- <div class="title">MBL MHC Tetramer</div> -->
249					<img src="images/section_6_1.png" alt="">
250					<p>Binding activity of the monomer to TCR is too weak to remain bound on the target T cell.  It is easily detached from the T cell.</p>
251				</div>
252				<div class="arrow">
253                    <div class="arrow-img"><img src="images/icn-arrow-right.png" alt="" class="hidden-sp"><img src="images/icn-arrow-down.png" alt="" class="hidden-pc"></div>
254                    <div class="arrow-text">Tetramerization</div>
255                </div>
256				<div class="right">
257					<!-- <div class="title">Monomer</div> -->
258					<img src="images/section_6_2.png" alt="">
259					<p>To overcome this difficulty, the monomer is biotinylated and tetramerized with a fluorescence-labeled streptavidin to increase avidity to TCR. It enables the tetramer to achieve stable binding to TCR on the antigen-specific T cell.</p>
260					<!-- <p>MHC/多肽复合物的单体(Monomer)与T细胞受体(TCR)的亲和性较低,无法稳定结合,因此无法用来检<br class="pc-768" />测抗原特异性T细胞(CTL)。</p> -->
261				</div>
262			</div>
263		</div>
264
265		<div class="section section-7" id="advantage">
266			<h2 class="title">Patented technology to increase specificity<div class="line"></div></h2>
267			<div class="box box-shadow">
268				<div class="left">
269					<!-- <div>非特异性结合位点<span>引入</span>突变</div> -->
270					<img src="images/section_7_1a.png" alt="">
271					<p>
271Our HLA Class I tetramer has a mutation in the &alpha;3 domain on HLA. It minimizes undesirable binding to CD8 molecules and dramatically improves specificity to detect target T cells.</p>
272					<a class="lt-btn" href="javascript:void(0);" class="more" data-lightbox="1" id="click_detail_with_TCR">
273						<img class="pc-414" src="images/more.png" alt="">
274						<img class="mb-414" src="images/mb_more.png" alt="">
275						<img class="on" src="images/more_on.png" alt="">
276					</a>
277				</div>
278				<div class="right">
279					<!-- <div>非特异性结合位点<span>没有引入</span>突变</div> -->
280					<img src="images/section_7_2a.png" alt="">
281					<p>CD8 is known to bind HLA to assist in TCR and HLA binding. This means that HLA contains a CD8-binding site.<br>
282					Therefore, it occasionally causes false positive signals between HLA tetramers and CD8-positive T cells.</p>
283					<a class="lt-btn" href="javascript:void(0);" class="more" data-lightbox="2" id="click_detail_without_TCR">
284						<img class="pc-414" src="images/more.png" alt="">
285						<img class="mb-414" src="images/mb_more.png" alt="">
286						<img class="on" src="images/more_on.png" alt="">
287					</a>
288				</div>
289				<div class="clear"></div>
290				<div class="center">
291					<p>*Please note that &alpha;3 mutation is adopted only in Human HLA Class I Tetramers, but not in other species including Mouse MHC Class I Tetramers.</p>
292				</div>
293			</div>
294			<!-- <p>( French Application Number FR9911133 )</p> -->
295		</div>
296
297		<div class="section section-8" id="faq">
298			<h2 class="title">Q&A<div class="line"></div></h2>
299			<div class="box">
300				<div class="qa">
301					<div class="question">
302						<span class="square">Q</span>
303						<p>How do I order MBL’s MHC Tetramers?</p>
304					</div>
305					<div class="answer">
306						<span class="square">A</span>
307						<p>We have a wide selection of class I (multiple species) and class II (human) alleles available for custom tetramer synthesis. Contact us with the allele, peptide sequence, and fluorochrome you wish to order. We recommend customers do a literature search to determine the best peptide/allele combination to detect the specific T cells of interest.<br><br>
308Our tetramer R&D manufacturing team will assess the theoretical feasibility (e.g. likelihood of successful tetramer manufacture) of that combination. Once the team determines feasibility, you will receive a quote with the price of the tetramer, as well as a projected lead-time.</p>
309					</div>
310				</div>
311
312				<div class="qa">
313					<div class="question">
314						<span class="square">Q</span>
315						<p>How long will it take for delivery?</p>
316					</div>
317					<div class="answer">
318						<span class="square">A</span>
319<p>Once your order is booked and a purchase order is received, you will be quoted a ship date by customer service. Delivery time is contingent on the type of tetramer you request.<br><br>Catalog : 3-30 days. Shipped next day if the tetramer is in stock, or 2-4 weeks if the monomer is available, but we have to prepare the tetramer.<br><br>Custom : Up to 60 days if peptide has to be ordered/synthesized. Can be produced in 3-4 weeks if we have the peptide on hand.</p>
320					</div>
321				</div>
322
323				<div class="qa">
324					<div class="question">
325						<span class="square">Q</span>
326						<p>Is there continuous after-sales service after purchase?</p>
327					</div>
328					<div class="answer">
329						<span class="square">A</span>
330						<p>Our technical support team in Japan will support your problem, and they strive to satisfactorily resolve your issue. Please feel free to ask any questions and concerns in use of our MHC tetramers.</p>
331					</div>
332				</div>
333
334				<div class="qa">
335					<div class="question">
336						<span class="square">Q</span>
337						<p>Which research fields are MHC tetramers used in?</p>
338					</div>
339					<div class="answer">
340						<span class="square">A</span>
341<p>MHC tetramers are used for the detection and monitoring of antigen-specific T cells to study the adaptive immune response, including T cell development, disease progression, and evaluation of therapeutics, vaccinations, and other interventions that may have an impact on cellular immune responses.  In clinical trials, MHC tetramers are used to monitor T cells that may be related to either the cause of illness (e.g. diabetes or autoimmune disease) or the fight against an ailment (e.g. cancer, infection).<br><br>There are numerous citations of MBL’s MHC tetramers worldwide.   
342Please refer to the citation list <a href="./pdf/T-Select_MHC_Tetramer_Citation_List_20181026.pdf" target="_blank" class="link-un
342der">here</a>.</p>
343					</div>
344				</div>
345
346			</div>
347		</div>
348
349		<div class="wechat view_in_target_04" id="scroll_lp_tetramer_cn_D">
350    		<div class="title">As a partner in your drug discovery and cancer immunotherapy</div>
351			<div class="row">
352    			<div class="col"><a class="btn-pdf" href="./tetramer_EN.pdf" target="_blank"><span>Brochures</span></a></div>
353    			<div class="col"><a class="btn-qq js-qq" href="https://ruo.mbl.co.jp/bio/g/inq/" target="_blank" id="qqBtn_cta04"><span>Contact us</span></a></div>
354			</div>
355		</div>
356
357		<div class="section section-9" id="make">
358			<h2 class="title">MHC Tetramer Custom Services<div class="line"></div></h2>
359			<div class="wrap">
360			<table>
361                <tbody>
362                    <tr>
363                        <th width="285">MHC Tetramer</th>
364                        <td>
365                            <div class="text">Various alleles for the following antigens are available.</div>
366                            <dl class="mhc">
367                                <dt>Cancer</dt>
368                                <dd>WT1 / gp100 / Mart-1 / p53 / Her-2</dd>
369                                <dt>Transplantation</dt>
370                                <dd>CMV / EBV / AdV / HSV / HHV / VZV</dd>
371                                <dt>Virus</dt>
372                                <dd>Influenza / HTLV-1 / HIV / HBV / HCV / HPV / M.tuberculosis</dd>
373                                <dt>Others</dt>
374                                <dd>Tetanus Toxin / OVA / MOG</dd>
375                            </dl>
376                        </td>
377                    </tr>
378                </tbody>
379            </table>
380			<table>
381                <tbody>
382                    <tr>
383                        <th width="285" rowspan="3">MHC Custom Tetramer</th>
384                        <td>
385							<div class="text">We can manufacture a custom MHC tetramer based on your needs (Over 60 alleles available).</div>
386							<ul>
387								<li>
388									<div class="title">Class I Tetramer Custom Service</div>
389									<p>Human / mouse / Rhesus Macaque / chicken</p>
390									<a class="ltbtn" href="javascript:void(0);" data-pos="1" id="click_detail_product_class1">
391										<img style="width: 206px;" src="images/allele_off.png" alt="">
392										<img style="width: 206px;" class="on" src="images/allele_on.png" alt="">
393									</a>
394								</li>
395								<li>
396									<div class="title">Class II Tetramer Custom Service</div>
397									<p>Human / mouse</p>
398									<a class="ltbtn" href="javascript:void(0);" data-pos="2" id="click_detail_product_class2">
399										<img style="width: 206px;" src="images/allele_off.png" alt="">
400										<img style="width: 206px;" class="on" src="images/allele_on.png" alt="">
401									</a>
402								</li>
403							</ul>
404                        </td>
405                    </tr>
406                    <tr>
407                        <td>
408							<div class="text">All-in-one kits to prepare custom tetramers in your laboratory.</div>
409							<ul>
410								<li>
411									<div class="title">QuickSwitch™ Custom Tetramer kit</div>
412								</li>
413							</ul>
414                        </td>
415                    </tr>
416                </tbody>
417            </table>
418			
419			<div class="board"><p><b>Related products</b>  <br class="mb-414" />Peptides loaded on MHC, CD8 antibodies</p></div>
420			<p class="text-center"><b>We provide numerous custom MHC alleles that can meet your needs worldwide.</b></p>
421			
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424        </div>
425
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469Copyright &copy; 2018 MEDICAL &amp; BIOLOGICAL LABORATORIES CO., LTD. All rights reserved.
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476	<div class="lightbox" id="lightbox-1">
477		<div class="close"><img src="images/close.png" alt=""></div>
478		<div class="title">Reducing Background<br>CD8-Staining<div class="line"></div></div>
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495		<div class="title">Causes non-Specific Binding<div class="line"></div></div>
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513		<div class="title mb-768">MHC Class I Custom Tetramer<div class="line"></div></div>
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525		<div class="title mb-768">MHC Class II Custom Tetramer<div class="line"></div></div>
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