https://matchboxscope.github.io/assets/js/ce60674a.76e66887.js

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1function u(e){let{components:t,...i}=e;return(0,r.yg)(g,(0,o.A)({},c,i,{components:t,mdxType:"MDXLayout"}),(0,r.yg)("h1",{id:"esp32-based-inline-holography-microscope"},"ESP32-based InLine Holography Microscope"),(0,r.yg)("p",null,"TEXT"),(0,r.yg)("h1",{id:"assembly"},"Assembly"),(0,r.yg)("p",null,"TEXT"),(0,r.yg)("h3",{id:"bill-of-material"},"Bill of material"),(0,r.yg)("table",null,(0,r.yg)("thead",{parentName:"table"},(0,r.yg)("tr",{parentName:"thead"},(0,r.yg)("th",{parentName:"tr",align:"center"},"Part"),(0,r.yg)("th",{parentName:"tr",align:"center"},"Purpose"),(0,r.yg)("th",{parentName:"tr",align:"center"},"Source"),(0,r.yg)("th",{parentName:"tr",align:"center"},"Price")))),(0,r.yg)("h2",{id:"3d-printing-files"},"3D printing files"),(0,r.yg)("p",null,"All design files can be found in the release from the ",(0,r.yg)("a",{parentName:"p",href:"https://github.com/Matchboxscope/Matchboxscope/releases/tag/ESPMicroscopeCollectionv2"},"Matchboxscope Releases")," (Look for miniplanktoscope)."),(0,r.yg)("p",null,"All 3D-printable files can be found ",(0,r.yg)("a",{parentName:"p",href:"./STL/Anglerfish_JAR"},"here"),":"),(0,r.yg)("h2",{id:"cad-files"},"CAD Files"),(0,r.yg)("p",null,"The release files can be found ",(0,r.yg)("a",{parentName:"p",href:"https://github.com/Matchboxscope/Matchboxscope/releases/download/ESPMicroscopeCollectionv2/CAD_PlanktoscopeMini.zip"},"https://github.com/Matchboxscope/Matchboxscope/releases/download/ESPMicroscopeCollectionv2/CAD_PlanktoscopeMini.zip")),(0,r.yg)("h2",{id:"assembly-process"},"Assembly Process"),(0,r.yg)("h2",{id:"reconstruction-code"},"Reconstruction code:"),(0,r.yg)("h3",{id:"online-based-pyscript"},"Online-based Pyscript"),(0,r.yg)("p",null,"Please visit the following website and upload your hologram: ",(0,r.yg)("a",{parentName:"p",href:"https://matchboxscope.github.io/pyscript/index_holo.html"},"https://matchboxscope.github.io/pyscript/index_holo.html"),"\nThe data won't leave your computer. Everything is implemented in pyodide/pyscript. The source code can be found here ",(0,r.yg)("a",{parentName:"p",href:"https://github.com/Matchboxscope/matchboxscope.github.io/blob/main/static/pyscript/image_upload_pillow.py"},"https://github.com/Matchboxscope/matchboxscope.github.io/blob/main/static/pyscript/image_upload_pillow.py")),(0,r.yg)("p",null,"As of now: The reconstruction distance is constant as I don't know how to make this a parameter. "),(0,r.yg)("h3",{id:"python"},"Python"),(0,r.yg)("pre",null,(0,r.yg)("code",{parentName:"pre",className:"language-py"},'#!/usr/bin/env python3\n# -*- coding: utf-8 -*-\n"""\nCreated on Thu Jun 22 22:00:20 2023\n\n@author: bene\n"""\n\n\nimport numpy as np\nimport matplotlib.pyplot as plt\nimport NanoImagingPack as nip\n\ndef reconstruct_inline_hologram(hologram, wavelength, ps, distance):\n\n# Inverse space\n     Nx = hologram.shape[1]\n     Ny = hologram.shape[0]\n     fx = np.linspace(-(Nx-1)/2*(1/(Nx*ps)), (Nx-1)/2*(1/(ps*Nx)), Nx)\n     fy = np.linspace(-(Ny-1)/2*(1/(ps*Ny)), (Ny-1)/2*(1/(ps*Ny)), Ny)\n     Fx, Fy = np.meshgrid(fx, fy)\n\n     kernel = np.exp(1j*(2 * np.pi / wavelength) * distance) * np.exp(1j * np.pi * wavelength * distance * (Fx**2 + Fy**2))\n\n     # Compute FFT centered about 0\n     E0fft = np.fft.fftshift(np.fft.fft2(hologram))\n\n     # Multiply spectrum with fresnel phase-factor\n     G = kernel*E0fft\n     Ef = np.fft.ifft2(np.fft.ifftshift(G))\n     return Ef\n\n\nimgHolo = plt.imread(\'/Users/bene/Downloads/ecoli.jpeg\')\nimgHolo = imgHolo/nip.gaussf(imgHolo, 25)\n\nfor iz in np.linspace(1,10,50):\n    img=reconstruct_inline_hologram(imgHolo[:,:,2], wavelength=450e-9, ps=5e-6 , distance=iz*1e-3)\n    plt.imshow(np.abs(img), cmap=\'gray\'), plt.title("dz"+str(iz*1e-3))\n    plt.savefig("ecoliDefocusEsp32"+str(iz)+".png")\n    plt.show()\n')),(0,r.yg)("h2",{id:"reults"},"Reults"),(0,r.yg)("p",null,"![](IMAGES/holoscope/git\n2023_11_14-Holo_Laser_USAF-1.gif)"),(0,r.yg)("p",null,(0,r.yg)("img",{src:n(224).A,width:"600",height:"360"})),(0,r.yg)("p",null,(0,r.yg)("img",{src:n(7329).A,width:"312",height:"312"})))}u.isMDXComponent=!0}

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