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3 </head> <body class="fixed-top-nav "> <header> <nav id="navbar" class="navbar navbar-light navbar-expand-sm fixed-top" role="navigation"> <div class="container"> <a class="navbar-brand title font-weight-lighter" href="/"> <span class="font-weight-bold">Mine</span> Altinli </a> <button class="navbar-toggler collapsed ml-auto" type="button" data-toggle="collapse" data-target="#navbarNav" aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation"> <span class="sr-only">Toggle navigation</span> <span class="icon-bar top-bar"></span> <span class="icon-bar middle-bar"></span> <span class="icon-bar bottom-bar"></span> </button> <div class="collapse navbar-collapse text-right" id="navbarNav"> <ul class="navbar-nav ml-auto flex-nowrap"> <li class="nav-item "> <a class="nav-link" href="/">about </a> </li> <li class="nav-item "> <a class="nav-link" href="/projects/">research interests </a> </li> <li class="nav-item active"> <a class="nav-link" href="/publications/">publications <span class="sr-only">(current)</span> </a> </li> <li class="nav-item "> <a class="nav-link" href="/culture/">shaping academic culture </a> </li> <li class="toggle-container"> <button id="light-toggle" title="Change theme"> <i class="ti ti-sun-moon" id="light-toggle-system"></i> <i class="ti ti-moon-filled" id="light-toggle-dark"></i> <i class="ti ti-sun-filled" id="light-toggle-light"></i> </button> </li> </ul> </div> </div> </nav> <progress id="progress" value="0"> <div class="progress-container"> <span class="progress-bar"></span> </div> </progress> </header> <div class="container mt-5" role="main"> <div class="post"> <header class="post-header"> <h1 class="post-title">publications</h1> <p class="post-description">publications by categories in reversed chronological order. generated by jekyll-scholar.</p> </header> <article> <div class="publications"> <h2 class="bibliography">2025</h2> <ol class="bibliography"> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="haba_ancient_2025" class="col-sm-8"> <div class="title">Ancient origin of an urban underground mosquito</div> <div class="author"> Yuki Haba, Matthew L. Aardema, Maria O. Afonso, Natasha M. Agramonte, John Albright, Ana Margarida Alho, Antonio P.G. Almeida, Haoues Alout, Bulent Alten, <em>Mine Altinli</em>, and <span class="more-authors" title="click to view 207 more authors" onclick=" var element=$(this); element.attr('title', ''); var more_authors_text=element.text() == '207 more authors' ? 'Raouf Amara Korba, Stefanos S. Andreadis, Vincent Anghel, Soukaina Arich, Arielle Ar
3senault-Benoit, Célestine Atyame, Fabien Aubry, Frank W. Avila, Diego Ayala, Rasha S. Azrag, Lilit Babayan, Allon Bear, Norbert Becker, Anna G. Bega, Sophia Bejarano, Ira Ben-Avi, Joshua B. Benoit, Saïd C. Boubidi, William E. Bradshaw, Daniel Bravo-Barriga, Rubén Bueno-Marí, Nataša Bušić, Viktoria Čabanová, Brittany Cabeje, Beniamino Caputo, Maria V. Cardo, Simon Carpenter, Elena Carreton, Mouhamadou S. Chouaïbou, Michelle Christian, Maureen Coetzee, William R. Conner, Anton Cornel, C. Lorna Culverwell, Aleksandra I. Cupina, Katrien De Wolf, Isra Deblauwe, Brittany Deegan, Sarah Delacour-Estrella, Alessandra Della Torre, Debora Diaz, Serena E. Dool, Vitor L Dos Anjos, Sisay Dugassa, Babak Ebrahimi, Samar Y.M. Eisa, Nohal Elissa, Sahar A.B. Fallatah, Ary Faraji, Marina V. Fedorova, Emily Ferrill, Dina M. Fonseca, Kimberly A. Foss, Cipriano Foxi, Caio M. França, Stephen R. Fricker, Megan L. Fritz, Eva Frontera, Hans-Peter Fuehrer, Kyoko Futami, Enas H.S. Ghallab, Romain Girod, Mikhail I. Gordeev, David Greer, Martin Gschwind, Milehna M. Guarido, Teoh Guat Ney, Filiz Gunay, Eran Haklay, Alwia A.E. Hamad, Jun Hang, Christopher M. Hardy, Jacob W. Hartle, Jenny C. Hesson, Yukiko Higa, Christina M. Holzapfel, Ann-Christin Honnen, Angela M. Ionica, Laura Jones, Përparim Kadriaj, Hany A. Kamal, Colince Kamdem, Dmitry A. Karagodin, Shinji Kasai, Mihaela Kavran, Emad I.M. Khater, Frederik Kiene, Heung-Chul Kim, Ilias Kioulos, Annette Klein, Marko Klemenčić, Ana Klobučar, Erin Knutson, Constantianus J.M. Koenraadt, Linda Kothera, Pauline Kreienbühl, Pierrick Labbé, Itay Lachmi, Louis Lambrechts, Nediljko Landeka, Christopher H. Lee, Bryan D. Lessard, Ignacio Leycegui, Jan O. Lundström, Yoav Lustigman, Caitlin MacIntyre, Andrew J. Mackay, Krisztian Magori, Carla Maia, Colin A. Malcolm, Ralph-Joncyn O. Marquez, Dino Martins, Reem A. Masri, Gillian McDivitt, Rebekah J. McMinn, Johana Medina, Karen S. Mellor, Jason Mendoza, Enrih Merdić, Stacey Mesler, Camille Mestre, Homer Miranda, Martina Miterpáková, Fabrizio Montarsi, Anton V. Moskaev, Tong Mu, Tim W.R. Möhlmann, Alice Namias, Ivy Ng’iru, Marc F. Ngangué, Maria T. Novo, Laor Orshan, José A. Oteo, Yasushi Otsuka, Rossella Panarese, Claudia Paredes-Esquivel, Lusine Paronyan, Steven T. Peper, Dušan V. Petrić, Kervin Pilapil, Cristina Pou-Barreto, Sebastien J. Puechmaille, Ute Radespiel, Nil Rahola, Vivek K Raman, Hamadouche Redouane, Michael H. Reiskind, Nadja M. Reissen, Benjamin L. Rice, Vincent Robert, Ignacio Ruiz-Arrondo, Ryan Salamat, Amy Salamone, M’hammed Sarih, Giuseppe Satta, Kyoko Sawabe, Francis Schaffner, Karen E. Schultz, Elena V. Shaikevich, Igor V. Sharakhov, Maria V. Sharakhova, Nader Shatara, Anuarbek K. Sibataev, Mathieu Sicard, Evan Smith, Ryan C. Smith, Nathalie Smitz, Nicolas Soriano, Christos G. Spanoudis, Christopher M. Stone, Liora Studentsky, Tatiana Sulesco, Luciano M. Tantely, La K. Thao, Noor Tietze, Ryan E. Tokarz, Kun-Hsien Tsai, Yoshio Tsuda, Nataša Turić, Melissa R. Uhran, Isik Unlu, Wim Van Bortel, Haykuhi Vardanyan, Laura Vavassori, Enkelejda Velo, Marietjie Venter, Goran Vignjević, Chantal B.F. Vogels, Tatsiana Volkava, John Vontas, Heather M. Ward, Nazni Wasi Ahmad, Mylène Weill, Jennifer D. West, Sarah S. Wheeler, Gregory S. White, Nadja C. Wipf, Tai-Ping Wu, Kai-Di Yu, Elke Zimmermann, Carina Zittra, Petra Korlević, Erica McAlister, Mara K.N. Lawniczak, Molly Schumer, Noah H. Rose, Carolyn S. McBride' : '207 more authors'; var cursorPosition=0; var textAdder=setInterval(function(){ element.html(more_authors_text.substring(0, cursorPosition + 1)); if (++cursorPosition == more_authors_text.length){ clearInterval(textAdder); } }, '10'); ">207 more authors</span> </div> <div class="periodical"> Jan 2025 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1101/2025.01.26.634793" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Abstract Understanding how life is adapting to urban environments represents an important challenge in evolutionary biology. Here we investigate a widely cited example of urban adaptation, Culex pipiens form molestus , also known as the London Underground Mosquito. Population genomic analysis of ∼350 contemporary and historical samples counter the popular hypothesis that molestus originated belowground in London less than 200 years ago. Instead, we show that molestus first adapted to human environments aboveground in the Middle East over the course of \textgreater1000 years, likely in concert with the rise of agricultural civilizations. Our results highlight the role of early human society in priming taxa for contemporary urban evolution and have important implications for understanding arbovirus transmission.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="boshra_detection_2025" class="col-sm-8"> <div class="title">Detection and Characterization of Antiviral Proteins Against Bunyaviruses in Mosquito-Derived Cells</div> <div class="author"> P. Böhmer, M. Reuter, <em>M. Altinli</em>, and E. Schnettler </div> <div class="periodical"> <em>In Bunyaviruses</em>, Jan 2025 </div> <div class="periodical"> Series Title: Methods in Molecular Biology </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1007/978-1-0716-4338-9_7" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Viruses of the class Bunyaviricetes are often transmitted by arthropods, including mosquitoes. The innate immune response in mosquitoes comprises several pathways, including sequence-specific degradation through RNA interference (RNAi). It is known that bunyavirus infections are targeted by the innate immune response in mosquitoes and derived cells; however, detailed information is often still missing. In this chapter, we describe a detailed protocol to determine if a protein of interest acts antiviral against bunyavirus infection in mosquito-derived cells. In line, we also describe a detailed protocol to investigate the effect of overexpression of a mosquito-encoded protein or analysis of mutant proteins on bunyavirus infection.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="jagtap_invasive_2025" class="col-sm-8"> <div class="title">Invasive Aedes albopictus is a competent vector for O’nyong Nyong virus</div> <div class="author"> Swati Jagtap, <em>Mine Altinli</em>, Marlis Badusche, Manuel Chevalier, Norbert Becker, Mayke Leggewie, and Esther Schnettler </div> <div class="periodical"> <em>One Health</em>, Jun 2025 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1016/j.onehlt.2025.101062" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>O’nyong-nyong virus (ONNV) is a zoonotic vector-borne alphavirus with humans as primary hosts during urban outbreaks. While its enzootic transmission cycle is not well investigated, its pattern of disappearance and ree­ mergence suggests overlooked but critical wildlife reservoirs. While ONNV travel-related cases were reported in Canada and Germany, autochthonous transmission is limited to sub-Saharan Africa. The lack of specialised vectors, such as anopheline mosquitoes, is considered to be one of the reasons. Here, we investigated the vector competence of native and invasive culicine mosquitoes from Germany. While the tested native culicine mosquitoes from Germany did not transmit ONNV, our results provide experimental evidence for potential ONNV transmission by invasive Ae. albopictus mosquitoes. We further investigated the role of temperature on ONNV transmission. Ae. albopictus mosquitoes are highly invasive and have become widespread in Europe in the last decade. Our results highlight that, combined with rising summer temperatures and the abundance of competent vectors, ONNV may emerge as a public health threat for Europe in the near future.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="altinli_bacteria-mediated_2025" class="col-sm-8"> <div class="title">Bacteria-mediated dsRNA delivery for mosquito-borne virus control</div> <div class="author"> <em>Mine Altinli</em>, Sean P. Leonard, Alain Kohl, and Grant L. Hughes </div> <div class="periodical"> <em>Trends in Parasitology</em>, Aug 2025 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1016/j.pt.2025.07.015" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Mosquito-borne viruses represent an increasing global public health threat, exacerbated by urbanisation and climate change, thus making effective mosquito control essential. RNA interference (RNAi), a sequence-specific gene regulation mechanism, can be a flexible vector control tool. RNAi effectors, such as double-stranded RNA (dsRNA), can target mosquito genes or the viruses they carry, disrupting development or suppressing infection. However, current RNAi delivery methods are ineffective. Engineered bacterial symbionts offer a promising alternative for delivery, as they can produce dsRNA directly within mosquitoes. However, bacterial RNAi delivery in mosquitoes remains underexplored. We review emerging genetic tools, insights from RNAi and bacteria–mosquito interactions to outline priorities for realising bacterial RNAi as an efficie
3nt and sustainable vector control strategy.</p> </div> </div> </div> </li> </ol> <h2 class="bibliography">2024</h2> <ol class="bibliography"><li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="williams_are_2024" class="col-sm-8"> <div class="title">Are Leibniz PostDocs alright? Insights into the careers, working lives, and mental health of Leibniz PostDocs</div> <div class="author"> Harry M. Williams, Regina Becker, Lena Roemer, <em>Mine Altinli</em>, Sabrina Sobieraj, Thomas Lösch, and Daniela Fiedler </div> <div class="periodical"> Aug 2024 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> </div> <div class="abstract hidden"> <p>Postdoctoral researchers play a critical role in scientific research. This is the case not only for the Leibniz Association but for research worldwide. However, postdoctoral researchers are often overlooked or misrepresented as a homogeneous group of researchers. The principal aim of the Leibniz PostDoc Survey was to understand who Leibniz PostDocs are and to provide some insight into their experiences, interests, and needs. This report summarises the results of the second Leibniz PostDoc Survey, which was open for submission from November 2022 to January 2023. Overall, we had questions covering the following sections: (1) demographics, (2) contract and payments, (3) working hours and tasks, (4) career goals, (5) career development – experiences, (6) career development – supervisor and institutional support, (7) satisfaction and working situation, (8) good scientific practice, workplace conflict, and discrimination, (9) mental health, as well as (10) the experience of international Leibniz PostDocs. The report includes a summary of the key findings and recommendations as well as a comprehensive description of all results from each survey question.</p> </div> </div> </div> </li></ol> <h2 class="bibliography">2023</h2> <ol class="bibliography"> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="altinli_antiviral_2023" class="col-sm-8"> <div class="title">Antiviral RNAi Response in Culex quinquefasciatus-Derived HSU Cells</div> <div class="author"> <em>Mine Altinli</em>, Mayke Leggewie, Jonny Schulze, Rashwita Gyanwali, Marlis Badusche, Vattipally B. Sreenu, Janina Fuss, and Esther Schnettler </div> <div class="periodical"> <em>Viruses</em>, Feb 2023 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.3390/v15020436" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Culex spp. mosquitoes are important vectors of viruses, such as West Nile virus, Eastern equine encephalitis virus and Rift valley fever virus. However, their interactions with innate antiviral immunity, especially RNA interference (RNAi), are not well known. Most research on RNAi pathways in mosquitoes is focused on the tropical vector mosquito Aedes aegypti. Here, we investigated the production of arbovirus-specific small RNAs in Cx. quinquefasciatus-derived HSU cells. Furthermore, by silencing RNAi-related proteins, we investigated the antiviral role of these proteins for two different arboviruses: Semliki Forest virus (SFV) and Bunyamwera orthobunyavirus (BUNV). Our results showed an expansion of Ago2 and Piwi6 in Cx. quinquefasciatus compared to Ae. aegypti. While silencing Ago2a and Ago2b increased BUNV replication, only Ago2b showed antiviral activity against SFV. Our results suggest differences in the function of Cx. quinquefasciatus and Ae. aegypti RNAi proteins and highlight the virus-specific function of these proteins in Cx. quinquefasciatus.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="jagtap_agua_2023" class="col-sm-8"> <div class="title">Agua Salud Alphavirus Infection, Dissemination and Transmission in Aedes aegypti Mosquitoes</div> <div class="author"> Swati V. Jagtap, Jorn Brink, Svea C. Frank, Marlis Badusche, Mayke Leggewie, Vattipally B. Sreenu, Janina Fuss, Esther Schnettler, and <em>Mine Altinli</em> </div> <div class="periodical"> <em>Viruses</em>, May 2023 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.3390/v15051113" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Mosquitoes are competent vectors for many important arthropod-borne viruses (arboviruses). In addition to arboviruses, insect-specific viruses (ISV) have also been discovered in mosquitoes. ISVs are viruses that replicate in insect hosts but are unable to infect and replicate in vertebrates. They have been shown to interfere with arbovirus replication in some cases. Despite the increase in studies on ISV–arbovirus interactions, ISV interactions with their hosts and how they are maintained in nature are still not well understood. In the present study, we investigated the infection and dissemination of the Agua Salud alphavirus (ASALV) in the important mosquito vector Aedes aegypti through different infection routes (per oral infection, intrathoracic injection) and its transmission. We show here that ASALV infects the female Ae. aegypti and replicates when mosquitoes are infected intrathoracically or orally. ASALV disseminated to different tissues, including the midgut, salivary glands and ovaries. However, we observed a higher virus load in the brain than in the salivary glands and carcasses, suggesting a tropism towards brain tissues. Our results show that ASALV is transmitted horizontally during adult and larval stages, although we did not observe vertical transmission. Understanding ISV infection and dissemination dynamics in Ae. aegypti and their transmission routes could help the use of ISVs as an arbovirus control strategy in the future.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="leggewie_aedes_2023" class="col-sm-8"> <div class="title">The Aedes aegypti RNA interference response against Zika virus in the context of co-infection with dengue and chikungunya viruses</div> <div class="author"> Mayke Leggewie, Christina Scherer, <em>Mine Altinli</em>, Rommel J. Gestuveo, Vattipally B. Sreenu, Janina Fuss, Marie Vazeille, Laurence Mousson, Marlis Badusche, Alain Kohl, and <span class="more-authors" title="click to view 2 more authors" onclick=" var element=$(this); element.attr('title', ''); var more_authors_text=element.text() == '2 more authors' ? 'Anna-Bella Failloux, Esther Schnettler' : '2 more authors'; var cursorPosition=0; var textAdder=setInterval(function(){ element.html(more_authors_text.substring(0, cursorPosition + 1)); if (++cursorPosition == more_authors_text.length){ clearInterval(textAdder); } }, '10'); ">2 more authors</span> </div> <div class="periodical"> <em>PLOS Neglected Tropical Diseases</em>, Jul 2023 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1371/journal.pntd.0011456" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Since its detection in 2015 in Brazil, Zika virus (ZIKV) has remained in the spotlight of international public health and research as an emerging arboviral pathogen. In addition to single infection, ZIKV may occur in co-infection with dengue (DENV) and chikungunya (CHIKV) viruses, with whom ZIKV shares geographic distribution and the mosquito Aedes aegypti as a vector. The main mosquito immune response against arboviruses is RNA interference (RNAi). It is unknown whether or not the dynamics of the RNAi response differ between single arboviral infections and co-infections. In this study, we investigated the interaction of ZIKV and DENV, as well as ZIKV and CHIKV co-infections with the RNAi response in Ae. aegypti. Using small RNA sequencing, we found that the efficiency of small RNA production against ZIKV -a hallmark of antiviral RNAi—was mostly similar when comparing single and co-infections with either DENV or CHIKV. Silencing of key antiviral RNAi proteins, showed no change in effect on ZIKV replication when the cell is co-infected with ZIKV and DENV or CHIKV. Interestingly, we observed a 
3negative effect on ZIKV replication during CHIKV coinfection in the context of Ago2-knockout cells, though his effect was absent during DENV co-infection. Overall, this study provides evidence that ZIKV single or co-infections with CHIKV or DENV are equally controlled by RNAi responses. Thus, Ae. aegypti mosquitoes and derived cells support co-infections of ZIKV with either CHIKV or DENV to a similar level than single infections, as long as the RNAi response is functional.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="agboli_interaction_2023" class="col-sm-8"> <div class="title">Interaction of Mesonivirus and Negevirus with arboviruses and the RNAi response in Culex tarsalis-derived cells</div> <div class="author"> Eric Agboli, Jonny Schulze, Stephanie Jansen, Daniel Cadar, Vattipally B. Sreenu, Mayke Leggewie, <em>Mine Altinli</em>, Marlis Badusche, Hanna Jöst, Jessica Börstler, and <span class="more-authors" title="click to view 2 more authors" onclick=" var element=$(this); element.attr('title', ''); var more_authors_text=element.text() == '2 more authors' ? 'Jonas Schmidt-Chanasit, Esther Schnettler' : '2 more authors'; var cursorPosition=0; var textAdder=setInterval(function(){ element.html(more_authors_text.substring(0, cursorPosition + 1)); if (++cursorPosition == more_authors_text.length){ clearInterval(textAdder); } }, '10'); ">2 more authors</span> </div> <div class="periodical"> <em>Parasites &amp; Vectors</em>, Oct 2023 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1186/s13071-023-05985-w" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Background  Mosquito-specific viruses (MSVs) comprise a variety of different virus families, some of which are known to interfere with infections of medically important arboviruses. Viruses belonging to the family Mesoniviridae or taxon Negevirus harbor several insect-specific viruses, including MSVs, which are known for their wide geographical distri‑bution and extensive host ranges. Although these viruses are regularly identified in mosquitoes all over the world, their presence in mosquitoes in Germany had not yet been reported. Methods  A mix of three MSVs (Yichang virus [Mesoniviridae] and two negeviruses [Daeseongdong virus and Dezi‑dougou virus]) in a sample that contained a pool of Coquillettidia richiardii mosquitoes collected in Germany was used to investigate the interaction of these viruses with different arboviruses in Culex-derived cells. In addition, small RNA sequencing and analysis of different mosquito-derived cells infected with this MSV mix were performed. Results  A strain of Yichang virus (Mesoniviridae) and two negeviruses (Daeseongdong virus and Dezidougou virus) were identified in the Cq. richiardii mosquitoes sampled in Germany, expanding current knowledge of their circula‑tion in central Europe. Infection of mosquito-derived cells with these three viruses revealed that they are targeted by the small interfering RNA (siRNA) pathway. In Culex-derived cells, co-infection by these three viruses had vary‑ing effects on the representative arboviruses from different virus families (Togaviridae: Semliki forest virus [SFV]; Bunyavirales: Bunyamwera orthobunyavirus [BUNV]; or Flaviviridae: Usutu virus [USUV]). Specifically, persistent MSV co-infection inhibited BUNV infection, as well as USUV infection (but the latter only at specific time points). However, the impact on SFV infection was only noticeable at low multiplicity of infection (MOI 0.1) and at specific time points in combination with the infection status. Conclusions  Taken together, these results are important findings that will lead to a better understanding of the com‑plex interactions of MSVs, mosquitoes and arboviruses.</p> </div> </div> </div> </li> </ol> <h2 class="bibliography">2022</h2> <ol class="bibliography"><li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="altinli_antiviral_2022" class="col-sm-8"> <div class="title">Antiviral RNAi Response against the Insect-Specific Agua Salud Alphavirus</div> <div class="author"> <em>Mine Altinli</em>, Mayke Leggewie, Marlis Badusche, Rashwita Gyanwali, Christina Scherer, Jonny Schulze, Vattipally B Sreenu, Marvin Fegebank, Bernhard Zibrat, Janina Fuss, and <span class="more-authors" title="click to view 2 more authors" onclick=" var element=$(this); element.attr('title', ''); var more_authors_text=element.text() == '2 more authors' ? 'Sandra Junglen, Esther Schnettler' : '2 more authors'; var cursorPosition=0; var textAdder=setInterval(function(){ element.html(more_authors_text.substring(0, cursorPosition + 1)); if (++cursorPosition == more_authors_text.length){ clearInterval(textAdder); } }, '10'); ">2 more authors</span> </div> <div class="periodical"> <em>mSphere</em>, Oct 2022 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1128/msphere.01003-21" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Arboviruses transmitted by mosquitoes are responsible for the death of millions of people each year. In addition to arboviruses, many insect-specific viruses (ISVs) have been discovered in mosquitoes in the last decade. ISVs, in contrast to arboviruses transmitted by mosquitoes to vertebrates, cannot replicate in vertebrate cells even when they are evolutionarily closely related to arboviruses. The alphavirus genus includes many arboviruses, although only a few ISVs have been discovered from this genus so far. Here, we investigate the interactions of a recently isolated insect-specific alphavirus, Agua Salud alphavirus (ASALV), with its mosquito host. RNA interference (RNAi) is one of the essential antiviral responses against arboviruses, although there is little knowledge on the interactions of RNAi with ISVs. Through the knockdown of transcripts of the different key RNAi pathway (small interfer
3ing RNA [siRNA], microRNA [miRNA], and P-element-induced wimpy testis [PIWI]-interacting RNA [piRNA]) proteins, we show the antiviral role of Ago2 (siRNA), Ago1 (miRNA), and Piwi4 proteins against ASALV in Aedes aegypti-derived cells. ASALV replication was increased in Dicer2 and Ago2 knockout cells, confirming the antiviral role of the siRNA pathway. In infected cells, mainly ASALV-specific siRNAs are produced, while piRNA-like small RNAs, with the characteristic nucleotide bias resulting from ping-pong amplification, are produced only in Dicer2 knockout cells. Taken together, ASALV interactions with the mosquito RNAi response differ from those of arthropod-borne alphaviruses in some aspects, although they also share some commonalities. Further research is needed to understand whether the identified differences can be generalized to other insect-specific alphaviruses.</p> </div> </div> </div> </li></ol> <h2 class="bibliography">2021</h2> <ol class="bibliography"> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="altinli_symbiotic_2021" class="col-sm-8"> <div class="title">Symbiotic Interactions Between Mosquitoes and Mosquito Viruses</div> <div class="author"> <em>Mine Altinli</em>, Esther Schnettler, and Mathieu Sicard </div> <div class="periodical"> <em>Frontiers in Cellular and Infection Microbiology</em>, Aug 2021 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.3389/fcimb.2021.694020" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Mosquitoes not only transmit human and veterinary pathogens called arboviruses (arthropod-borne viruses) but also harbor mosquito-associated insect-specific viruses (mosquito viruses) that cannot infect vertebrates. In the past, studies investigating mosquito viruses mainly focused on highly pathogenic interactions that were easier to detect than those without visible symptoms. However, the recent advances in viral metagenomics have highlighted the abundance and diversity of viruses which do not generate mass mortality in host populations. Over the last decade, this has facilitated the rapid growth of virus discovery in mosquitoes. The circumstances around the discovery of mosquito viruses greatly affected how they have been studied so far. While earlier research mainly focused on the pathogenesis caused by DNA and some doublestranded RNA viruses during larval stages, more recently discovered single-stranded RNA mosquito viruses were heavily studied for their putative interference with arboviruses in female adults. Thus, many aspects of mosquito virus interactions with their hosts and host-microbiota are still unknown. In this context, considering mosquito viruses as endosymbionts can help to identify novel research areas, in particular in relation to their long-term interactions with their hosts (e.g. relationships during all life stages, the stability of the associations at evolutionary scales, transmission routes and virulence evolution) and the possible context-dependent range of interactions (i.e. beneficial to antagonistic). Here, we review the symbiotic interactions of mosquito viruses considering different aspects of their ecology, such as transmission, host specificity, host immune system and interactions with other symbionts within the host cellular arena. Finally, we highlight related research gaps in mosquito virus research.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="sicard_cytoplasmic_2021" class="col-sm-8"> <div class="title">Cytoplasmic Incompatibility Variations in Relation with Wolbachia cid Genes Divergence in Culex pipiens</div> <div class="author"> Mathieu Sicard, Alice Namias, Marco Perriat-Sanguinet, Eric Carron, Sandra Unal, <em>Mine Altinli</em>, Frederic Landmann, and Mylène Weill </div> <div class="periodical"> <em>mBio</em>, Feb 2021 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1128/mBio.02797-20" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>In arthropods, Wolbachia endosymbionts induce conditional sterility, called cytoplasmic incompatibility (CI), resulting from embryonic lethality. CI penetrance (i.e., embryonic death rate) varies depen
3ding on host species and Wolbachia strains involved. All Culex pipiens mosquitoes are infected by the endosymbiotic alphaproteobacteria Wolbachia wPip. CI in Culex, characterized as a binary “compatible/incompatible” phenomenon, revealed an unparalleled diversity of patterns linked to the amplification-diversification of cidA and cidB genes. Here, we accurately studied CI penetrance variations in the light of cid genes divergence by generating a C. pipiens compatibility matrix between 11 lines hosting different phylogenetic wPip groups and exhibiting distinct cid gene repertoires. We showed, as expected, that crosses involving wPip from the same group were mostly compatible. In contrast, only 22% of the crosses involving different wPip groups were compatible, while 54% were fully incompatible. For the remaining 24% of the crosses, “intermediate” compatibilities were reported, and a cytological observation of the first zygotic division confirmed the occurrence of “canonical” CI phenotypes in a fraction of the eggs. Backcross experiments demonstrated that intermediate compatibilities were not linked to host genetic background but to the Wolbachia strains involved. This previously unstudied intermediate penetrance CI was more severe and frequent in crosses involving wPip-IV strains exhibiting cid variants markedly divergent from other wPip groups. Our data demonstrate that CI is not always a binary compatible/incompatible phenomenon in C. pipiens but that intermediate compatibilities putatively resulting from partial mismatch due to Cid proteins divergence exist in this species complex.</p> </div> </div> </div> </li> </ol> <h2 class="bibliography">2020</h2> <ol class="bibliography"><li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="altinli_wolbachia_2020" class="col-sm-8"> <div class="title">Wolbachia modulates prevalence and viral load of Culex pipiens densoviruses in natural populations</div> <div class="author"> <em>Mine Altinli</em>, Sebastian Lequime, Celestine Atyame, Fabienne Justy, Mylene Weill, and Mathieu Sicard </div> <div class="periodical"> <em>Molecular Ecology</em>, Oct 2020 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1111/mec.15609" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>The inadequacy of standard mosquito control strategies calls for ecologically safe novel approaches, for example the use of biological agents such as the endosymbiotic α-proteobacteria Wolbachia or insect-specific viruses (ISVs). Understanding the ecological interactions between these “biocontrol endosymbionts” is thus a fundamental step. Wolbachia are transmitted vertically from mother to offspring and modify their hosts’ phenotypes, including reproduction (e.g., cytoplasmic incompatibility) and survival (e.g., viral interference). In nature, Culex pipiens (sensu lato) mosquitoes are always foun
3d infected with genetically diverse Wolbachia called wPip that belong to five phylogenetic groups. In recent years, ISVs have also been discovered in these mosquito species, although their interactions with Wolbachia in nature are unknown. Here, we studied the interactions between a widely prevalent ISV, the Culex pipiens densovirus (CpDV, Densovirinae), and Wolbachia in northern Tunisian C. pipiens populations. We showed an influence of different Wolbachia groups on CpDV prevalence and a general positive correlation between Wolbachia and CpDV loads. By investigating the putative relationship between CpDV diversification and wPip groups in the different sites, we detected a signal linked to wPip groups in CpDV phylogeny in sites where all larvae were infected by the same wPip group. However, no such signal was detected where the wPip groups coexisted, suggesting CpDV horizontal transfer between hosts. Overall, our results provide good evidence for an ecological influence of Wolbachia on an ISV, CpDV, in natural populations and highlight the importance of integrating Wolbachia in our understanding of ISV ecology in nature.</p> </div> </div> </div> </li></ol> <h2 class="bibliography">2019</h2> <ol class="bibliography"> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="agboli_mosquito-specific_2019" class="col-sm-8"> <div class="title">Mosquito-Specific Viruses—Transmission and Interaction</div> <div class="author"> Agboli,  Leggewie,  Altinli, and  Schnettler </div> <div class="periodical"> <em>Viruses</em>, Sep 2019 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.3390/v11090873" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Mosquito-specific viruses (MSVs) are a subset of insect-specific viruses that are found to infect mosquitoes or mosquito derived cells. There has been an increase in discoveries of novel MSVs in recent years. This has expanded our understanding of viral diversity and evolution but has also sparked questions concerning the transmission of these viruses and interactions with their hosts and its microbiome. In fact, there is already evidence that MSVs interact with the immune system of their host. This is especially interesting, since mosquitoes can be infected with both MSVs and arthropod-borne (arbo) viruses of public health concern. In this review, we give an update on the different MSVs discovered so far and describe current data on their transmission and interaction with the mosquito immune system as well as the effect MSVs could have on an arboviruses-coinfection. Lastly, we discuss potential uses of these viruses, including vector and transmission control.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="altinli_evolution_2019" class="col-sm-8"> <div class="title">Evolution and phylogeography of Culex pipiens densovirus</div> <div class="author"> <em>Mine Altinli</em>, Sebastian Lequime, Maxime Courcelle, Sarah François, Fabienne Justy, Anne-Sophie Gosselin-Grenet, Mylene Ogliastro, Mylene Weill, and Mathieu Sicard </div> <div class="periodical"> <em>Virus Evolution</em>, Jul 2019 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1093/ve/vez053" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Viruses of the Parvoviridae family infect a wide range of animals including vertebrates and invertebrates. So far, our understanding of parvovirus diversity is biased towards medically or economically important viruses mainly infecting vertebrate hosts, while invertebrate infecting parvoviruses—namely densoviruses—have been largely neglected. Here, we investigated the prevalence and the evolution of the only mosquito-infecting ambidensovirus, Culex pipiens densovirus (CpDV), from laboratory mosquito lines and natural populations collected worldwide. CpDV diversity generally grouped in two clades, here named CpDV-1 and -2. The incongruence of the different gene trees for some samples suggested the possibility of recombination events between strains from different clades. We further investigated the role of selection on the evolution of CpDV genome and detected many individual sites under purifying selection both in non-structural and structural genes. However, some sites in structural genes were under diversifying selection, especially during the divergence of CpDV-1 and -2 clades. These substitutions between CpDV-1 and -2 clades were mostly located in the capsid protein encoding region and might cause changes in host specificity or pathogenicity of CpDV strains from the two clades. However, additional functional and experimental studies are necessary to fully understand the protein conformations and the resulting phenotype of these substitutions between clades of CpDV.</p> </div> </div> </div> </li> <li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="altinli_sharing_2019" class="col-sm-8"> <div class="title">Sharing cells with Wolbachia : the transovarian vertical transmission of Culex pipiens densovirus</div> <div class="author"> <em>Mine Altinli</em>, Julien Soms, Marc Ravallec, Fabienne Justy, Manon Bonneau, Mylene Weill, Anne‐Sophie Gosselin‐Grenet, and Mathieu Sicard </div> <div class="periodical"> <em>Environmental Microbiology</em>
3, Sep 2019 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1111/1462-2920.14511" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Culex pipiens densovirus (CpDV), a single stranded DNA virus, has been isolated from Culex pipiens mosquitoes but differs from other mosquito densoviruses in terms of genome structure and sequence identity. Its transmission from host to host, the nature of its interactions with both its host and host’s endosymbiotic bacteria Wolbachia are not known. Here, we report the presence of CpDV in the ovaries and eggs of Cx. pipiens mosquitoes in close encounters with Wolbachia. In the ovaries, CpDV amount significantly differed between mosquito lines harbouring different strains of Wolbachia and these differences were not linked to variations in Wolbachia densities. CpDV was vertically transmitted in all laboratory lines to 17%–20% of the offspring. For some females, however, the vertical transmission reached 90%. Antibiotic treatment that cured the host from Wolbachia significantly decreased both CpDV quantity and vertical transmission suggesting an impact of host microbiota, including Wolbachia, on CpDV transmission. Overall our results show that CpDV is transmitted vertically via transovarian path along with Wolbachia with which it shares the same cells. Our results are primordial to understand the dynamics of densovirus infection, their persistence and spread in populations considering their potential use in the regulation of mosquito vector populations.</p> </div> </div> </div> </li> </ol> <h2 class="bibliography">2018</h2> <ol class="bibliography"><li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="altinli_wolbachia_2018" class="col-sm-8"> <div class="title">Wolbachia diversity and cytoplasmic incompatibility patterns in Culex pipiens populations in Turkey</div> <div class="author"> <em>Mine Altinli</em>, Filiz Gunay, Bulent Alten, Mylene Weill, and Mathieu Sicard </div> <div class="periodical"> <em>Parasites &amp; Vectors</em>, Dec 2018 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.1186/s13071-018-2777-9" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Background: Wolbachia are maternally transmitted bacteria that can manipulate their hosts’ reproduction causing cytoplasmic incompatibility (CI). CI is a sperm-egg incompatibility resulting in embryonic death. Due to this sterilising effect on mosquitoes, Wolbachia are considered for vector control strategies. Important vectors for arboviruses, filarial nematodes and avian malaria, mosquitoes of Culex pipiens complex are suitable for Wolbachia-based vector control. They are infected with Wolbachia wPip strains belonging to five genetically distinct groups (wPip-I to V) within the Wolbachia B supergroup. CI properties of wPip strongly correlate with this genetic diversity: mosquitoes infected with wPip strains from a different wPip group are more likely to be incompatible with each other. Turkey is a critical spot for vector-borne diseases due to its unique geographical position as a natural bridge between Asia, Europe and Africa. However, general wPip diversity, distribution and CI patterns in natural Cx. pipiens (s.l.) populations in the region are unknown. In this study, we first identified wPip diversity in Turkish Cx. pipiens (s.l.) populations, by assigning them to one of the five groups within wPip (wPip-Ito V). We further investigated CI properties between different wPip strains from this region. Results: We showed a wPip fixation in Cx. pipiens (s.l.) populations in Turkey by analysing 753 samples from 59 sampling sites. Three wPip groups were detected in the region: wPip-I, wPip-II and wPip-IV. The most dominant group was wPip-II. While wPip-IV was restricted to only two locations, wPip-I and wPip-II had wider distributions. Individuals infected with wPip-II were found co-existing with individuals infected with wPip-I or wPip-IV in some sampling sites. Two mosquito isofemale lines harbouring either a wPip-I or a wPip-II strain were established from a population in northwestern Turkey. Reciprocal crosses between these lines showed that they were fully compatible with each other but bidirectionally incompatible with wPip-IV Istanbul infected line. Conclusion: Our findings reveal a high diversity of wPip and CI properties in Cx. pipiens (s.l.) populations in Turkey. Knowledge on naturally occurring CI patterns caused by wPip diversity in Turkey might be useful for Cx. pipiens (s.l.) control in the region.</p> </div> </div> </div> </li></ol> <h2 class="bibliography">2015</h2> <ol class="bibliography"><li> <div class="row"> <div class="col col-sm-2 abbr"> </div> <div id="braquart-varnier_mutualistic_2015" class="col-sm-8"> <div class="title">The Mutualistic Side of Wolbachia–Isopod Interactions: Wolbachia Mediated Protection Against Pathogenic Intracellular Bacteria</div> <div class="author"> Christine Braquart-Varnier, <em>Mine Altinli</em>, Romain Pigeault, Frédéric D. Chevalier, Pierre Grève, Didier Bouchon, and Mathieu Sicard </div> <div class="periodical"> <em>Frontiers in Microbiology</em>, Dec 2015 </div> <div class="periodical"> </div> <div class="links"> <a class="abstract btn btn-sm z-depth-0" role="button">Abs</a> <a href="https://doi.org/10.3389/fmicb.2015.01388" class="btn btn-sm z-depth-0" role="button" rel="external nofollow noopener" target="_blank">DOI</a> </div> <div class="abstract hidden"> <p>Wolbachia is a vertically transmitted endosymbiont whose radiative success is mainly related to various host reproductive manipulations that led to consider this symbiont as a conflictual reproductive parasite. However, lately, some Wolbachia have been shown to act as beneficial symbionts by protecting hosts against a broad range of parasites. Still, this protection has been mostly demonstrated in artificial Wolbachia-host associations between partners that did not co-evolved together. Here, we tested in two terrestrial isopod species Armadillidium vulgare and Porcellio dilatatus whether resident Wolbachia (native or non-native) could confer protection during infections with Listeria ivanovii and Salmonella typhimurium and also during a transinfection with a Wolbachia strain that kills the recipient host (i.e., wVulC in P. dilatatus). Survival analyses showed that (i) A. vulgare lines hosting their native Wolbachia (wVulC) always exhibited higher survival than asymbiotic ones when infected with pathogenic bacteria (ii) P. dilatatus lines hosting their native wDil Wolbachia strain survived the S. typhimurium infection better, while lines hosting non-native wCon Wolbachia strain survived the L. ivanovii and also the transinfection with wVulC from A. vulgare better. By studying L. ivanovii and S. typhimurium loads in the hemolymph of the different host-Wolbachia systems, we showed that (i) the difference in survival between lines after L. ivanovii infections were not linked to the difference between their pathogenic bacterial loads, and (ii) the difference in survival after S. typhimurium infections corresponds to lower loads of pathogenic bacteria. Overall, our results demonstrate a beneficial effect of Wolbachia on survival of terrestrial isopods when infected with pathogenic intracellular bacteria. This protective effect may rely on different mechanisms depen
3ding on the resident symbiont and the invasive bacteria interacting together within the hosts.</p> </div> </div> </div> </li></ol> </div> </article> </div> </div> <footer class="fixed-bottom" role="contentinfo"> <div class="container mt-0"> © Copyright 2025 Mine Altinli. </div> </footer> 
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