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74    {"@context":"http:\/\/schema.org\/","@id":"\/products\/iptg#product","@type":"ProductGroup","brand":{"@type":"Brand","name":"GoldBio"},"category":"Laboratory Chemicals","description":"IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.\nIPTG is commonly used in cloning procedures that require induction of β-galactosidase and is most often used with X-Gal (Gold Bio #X4281C) or Bluo-Gal (Gold Bio #B-673) for blue\/white colony screening or Magenta-Gal (Gold Bio #B-378) for red\/white colony screening of bacterial colonies.\nIPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).\nGoldBio IPTG is made from a synthetic, non-animal origin.\n \nFunctional Highlights and Mechanism\n\n\nIPTG acts by mimicking the natural inducer allolactose: it binds to the lac repressor (LacI) and causes its release from the lac operator site, allowing transcription of downstream genes under the lac promoter.\n\n\nBecause IPTG is not metabolized by the cell, its concentration remains stable over time, enabling sustained induction rather than being depleted. \n\n\nIn recombinant protein expression systems (for example vectors using T7 promoter under lac operon regulation), addition of IPTG triggers expression of the gene of interest downstream of the inducible promoter. \n\n\nIPTG is often used in colony screening (e.g., blue\/white screening) in combination with chromogenic substrates such as X‑Gal: in this context IPTG activates β-galactosidase expression which cleaves X-Gal to yield a colored product. \n\n\nBecause of the consistency and high quality of GoldBio’s IPTG, it supports reproducible gene induction workflows and reduces variability associated with induction reagent quality.\n\n\n \nApplications\n\nGene expression induction in systems controlled by the lac operon\nBlue-white screening (with X-Gal) for recombinant DNA identification\nControlled production of recombinant proteins\nTimed gene activation for functional studies\nRegulatory input in synthetic biology circuits\n\nResearch Applications for IPTG\n(Click each for more information)\n\nInduction of Protein Expression in Lac Operon-Based Systems \n\n\nPurpose: To induce recombinant protein expression in bacterial systems, particularly Escherichia coli.\n\nHow It Works: IPTG is a non-metabolizable analog of allolactose that binds to the LacI repressor, causing dissociation from the lac operator and activation of transcription from lac-derived promoters.\n\nApplications: High-yield recombinant protein production using pET, pGEX, pMAL, and related expression systems.\n\nStudier, F. W., \u0026amp; Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. Journal of Molecular Biology, 189(1), 113–130.\n\n\nBlue\/White Screening in Cloning \n\n\nPurpose: To differentiate recombinant clones from non-recombinant colonies during cloning workflows.\n\nHow It Works: IPTG induces β-galactosidase expression; in the presence of X-gal, colonies with intact lacZα appear blue, while recombinant colonies with disrupted lacZ remain white.\n\nApplications: Screening workflows using pUC19, pBluescript, and other multiple cloning site-containing vectors.\n\nVieira, J., \u0026amp; Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene, 19(3), 259–268.\n\n\nStudy of Gene Regulation and Promoter Function \n\n\nPurpose: To investigate transcriptional regulation and promoter-controlled gene expression.\n\nHow It Works: IPTG induces reporter genes such as GFP or luciferase downstream of lac-based promoters, enabling quantitative analysis of promoter activity and regulatory dynamics.\n\nApplications: Promoter mapping, transcriptional dynamics studies, functional genomics, and educational laboratory assays.\n\nZaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., ... \u0026amp; Alon, U. (2006). A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nature Methods, 3(8), 623–628.\n\n\nMetabolic Engineering and Pathway Control \n\n\nPurpose: To enable temporal regulation of enzyme expression in engineered metabolic pathways.\n\nHow It Works: Genes encoding pathway enzymes are placed under lac-derived promoters, allowing controlled pathway activation and metabolic flux modulation through IPTG induction.\n\nApplications: Production optimization for biofuels, amino acids, polymers, and specialty chemicals in engineered microbial systems.\
74n\nZhang, F., Carothers, J. M., \u0026amp; Keasling, J. D. (2012). Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. Nature Biotechnology, 30(4), 354–359.\n\n\nConditional Gene Expression in Synthetic Biology \n\n\nPurpose: To provide external control over synthetic gene circuits and regulatory networks.\n\nHow It Works: LacI-IPTG regulatory modules function as tunable switches, repressors, or oscillators within engineered biological systems.\n\nApplications: Synthetic circuit design, biosensor development, microbial consortia regulation, and educational synthetic biology platforms.\n\nElowitz, M. B., \u0026amp; Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. Nature, 403(6767), 335–338.\n\n \nReconstitution\n100mM IPTG stock solution\n\nWeigh 0.238 g of IPTG\nAdd 10 mL sterile H2O. Dissolve completely.\nPrewet a 0.22 µm syringe filter by drawing through 5-10 mL of sterile H2O and discard water.\nSterilize IPTG Stock through the prepared 0.22 µm syringe filter.\nStore in 1 mL aliquots at -20°C for up to 1 year.\n\n \nStorage\/Handling\nStore powder IPTG desiccated at -20°C. Protect from light. For IPTG stock solution, store in 1 mL aliquots at -20°C for up to 1 year. IPTG is readily dissolved in sterile water.\n \nFAQ\nHow does IPTG work?\nIPTG, which is similar to the lactose metabolite allolactose, induces the lac operon by binding to the lac repressor. Once IPTG binds to the lac repressor, a conformational change occurs, causing the lac repressor to dissociate from the lac promoter. Once the lac repressor dissociates, RNA polymerase binds to the promoter to initiate expression.\nHow much IPTG should be used for expression\/ What concentration of IPTG should be used for expression?\nMost papers recommend using between 0.1-1.0mM IPTG for expression. However, the amount you should use depends on your proteins. However, it is best to run an IPTG titration with ranges between 0.01-1.0mM and compare using SDS-PAGE to determine the optimal concentration. Something to keep in mind is higher concentrations of IPTG can cause inclusion bodies.\nWhy should I use IPTG over lactose?\nIPTG is non-metabolizable, so its concentration remains stable and doesn’t get consumed by the bacteria.\nWhen should I add IPTG during culture growth?\nYou should usually add IPTG at the mid-log phase (OD600 ≈ 0.4–0.6) to ensure cells are healthy and divi
74ding rapidly.\nDo I need to autoclave IPTG?\n\nNo—filter sterilize only. IPTG is heat-sensitive and should not be autoclaved.\nDoes IPTG degrade over time?\n\nIPTG is stable when stored properly (aliquoted at -20°C, protected from light), but repeated freeze-thaw cycles should be avoided.\nCan IPTG be reused after thawing?\n\nThe best practice is to use single-use aliquots of IPTG. Do not refreeze IPTG leftovers to avoid degradation or contamination.\nCan IPTG be toxic to cells?\n\nIPTG can be toxic at high concentrations or in sensitive strains. Lowering the IPTG concentration or using auto-induction media can help.\n\nIPTG Tutorial Videos\n   ","hasVariant":[{"@id":"\/products\/iptg?variant=44854634610745#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 1 g","offers":{"@id":"\/products\/iptg?variant=44854634610745#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"27.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634610745"},"sku":"I2481C"},{"@id":"\/products\/iptg?variant=44854634643513#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 5 g","offers":{"@id":"\/products\/iptg?variant=44854634643513#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"38.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634643513"},"sku":"I2481C5"},{"@id":"\/products\/iptg?variant=44854634676281#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 25 g","offers":{"@id":"\/products\/iptg?variant=44854634676281#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"153.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634676281"},"sku":"I2481C25"},{"@id":"\/products\/iptg?variant=44854634709049#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 50 g","offers":{"@id":"\/products\/iptg?variant=44854634709049#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"237.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634709049"},"sku":"I2481C50"},{"@id":"\/products\/iptg?variant=44854634741817#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 100 g","offers":{"@id":"\/products\/iptg?variant=44854634741817#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"437.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634741817"},"sku":"I2481C100"},{"@id":"\/products\/iptg?variant=44854634774585#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 200 g","offers":{"@id":"\/products\/iptg?variant=44854634774585#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"836.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634774585"},"sku":"I2481C200"},{"@id":"\/products\/iptg?variant=44854634807353#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 300 g","offers":{"@id":"\/products\/iptg?variant=44854634807353#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"1113.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634807353"},"sku":"I2481C300"},{"@id":"\/products\/iptg?variant=44854634840121#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 400 g","offers":{"@id":"\/products\/iptg?variant=44854634840121#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"1446.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634840121"},"sku":"I2481C400"},{"@id":"\/products\/iptg?variant=44854634872889#variant","@type":"Product","image":"https:\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620\u0026width=1920","name":"IPTG - 500 g","offers":{"@id":"\/products\/iptg?variant=44854634872889#offer","@type":"Offer","availability":"http:\/\/schema.org\/InStock","price":"1740.00","priceCurrency":"USD","url":"https:\/\/www.goldbio.com\/products\/iptg?variant=44854634872889"},"sku":"I2481C500"}],"name":"IPTG","productGroupID":"8011355488313","url":"https:\/\/www.goldbio.com\/products\/iptg"}
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689<script>(function () {var userAgent = navigator.userAgent;var platform = navigator.platform;var maxTouchPoints = navigator.maxTouchPoints || 0;var isIOS = /iPad|iPhone|iPod/.test(platform) || (platform === 'MacIntel' && maxTouchPoints > 1);var isMacSafari = platform.indexOf('Mac') === 0 && /Safari/.test(userAgent) && !/Chrome|Chromium|CriOS|FxiOS|Edg|OPR|Android/.test(userAgent);var isAppleSafari = isIOS || isMacSafari;if (isAppleSafari) {fetch('/sf_private_access_tokens' + location.search).catch(function () {});}function browserMajorVersion(pattern) {var match = userAgent.match(pattern);return match ? parseInt(match[1], 10) : null;}function shouldLoadAutosizesPolyfill() {if (!window.PerformanceObserver?.supportedEntryTypes?.includes('paint')) {return false;}var chromeVersion = browserMajorVersion(/Chrome\/(\d+)/);if (chromeVersion !== null) {return chromeVersion < 126;}var firefoxVersion = browserMajorVersion(/Firefox\/(\d+)/);if (firefoxVersion !== null) {return firefoxVersion < 150;}var safariVersion = isAppleSafari ? browserMajorVersion(/Version\/(\d+).*Safari\//) : null;if (safariVersion !== null) {return safariVersion < 27;}return true;}if (shouldLoadAutosizesPolyfill()) {var autosizesScript = document.createElement('script');autosizesScript.async = true;autosizesScript.crossOrigin = 'anonymous';autosizesScript.src = "//www.goldbio.com/cdn/shopifycloud/storefront/assets/storefront/autosizes-84416378.js";(document.head || document.documentElement).appendChild(autosizesScript);}window.ShopifyAnalytics = window.ShopifyAnalytics || {};window.ShopifyAnalytics.performance = window.ShopifyAnalytics.performance || {};(function () {var LONG_FRAME_THRESHOLD = 50;var longAnimationFrames = [];var activeRafId = null;function collectLongFrames() {var previousTime = null;function rafMonitor(now) {if (activeRafId === null) {return;}var delta = now - previousTime;if (delta > LONG_FRAME_THRESHOLD) {longAnimationFrames.push({startTime: previousTime,endTime: now,});}previousTime = now;activeRafId = requestAnimationFrame(rafMonitor);}previousTime = performance.now();activeRafId = requestAnimationFrame(rafMonitor);}if (!window.PerformanceObserver?.supportedEntryTypes?.includes('long-animation-frame')) {collectLongFrames();var timeoutId = setTimeout(function () {cancelAnimationFrame(activeRafId);}, 10000);window.ShopifyAnalytics.performance.getLongAnimationFrames = function (stopCollection) {if (stopCollection === undefined) {stopCollection = false;}if (stopCollection) {clearTimeout(timeoutId);cancelAnimationFrame(activeRafId);}return longAnimationFrames;};}})();})();</script>
689<script id="shopify-origin-trials" async="async" integrity="sha256-qJbe/jEZBHtr5W/BlA2cKAz3vkXu6vJQyLjgvgCC86g=" src="//cdn.shopify.com/shopifycloud/storefront/assets/storefront/origin_trials-318ab40a.js" crossorigin="anonymous" onload="window.__shopifyOriginTrialsDone = true" onerror="window.__shopifyOriginTrialsDone = true"></script>
vendor: 1 bytes, line 689
689
690<script>
691  window.Shopify = window.Shopify || {};
692  window.Shopify.MCP = window.Shopify.MCP || {};
693  window.Shopify.MCP.enabled = true;
694  window.Shopify.MCP.shop = "9t079h-bv.myshopify.com";
695  window.Shopify.MCP.mcpEndpoint = "https:\/\/www.goldbio.com\/api\/mcp";
696  window.Shopify.MCP.tools = [{"name":"search_shop_policies_and_faqs","description":"Used to get facts about the stores policies, products, or services.\nSome examples of questions you can ask are:\n  - What is your return policy?\n  - What is your shipping policy?\n  - What is your phone number?\n  - What are your hours of operation?\"\n","inputSchema":{"$schema":"https:\/\/json-schema.org\/draft\/2020-12\/schema","type":"object","properties":{"query":{"type":"string","description":"A natural language query."},"context":{"type":"string","description":"Additional information about the request such as user demographics, mood, location, or other relevant details that could help in tailoring the response appropriately."}},"required":["query"]}}];
697</script>
vendor: 1 bytes, line 697
697
698<script>(()=>{var d="shopify:webmcp_adapter_loaded",n=Symbol.for("shopify.webmcp_adapter_loading");function s(c,a,{win:r=window,doc:o=document}={}){function p(){try{return r.localStorage.getItem(d)==="true"}catch{return!1}}function l(){try{r.localStorage.setItem(d,"true")}catch{}}function u(){return typeof(o.modelContext||r.navigator?.modelContext)?.registerTool=="function"}function f(){if(r[n]||!u())return;let t=o.head||o.getElementsByTagName("head")[0];if(!t)return;let e=o.createElement("script");e.type="module",e.crossOrigin="anonymous",a&&(e.integrity=a),e.src=c,e.addEventListener("load",l,{once:!0}),e.addEventListener("error",()=>{r[n]=!1},{once:!0}),t.appendChild(e),r[n]=!0}function _(t){let e=o.getElementById("shopify-origin-trials");if(!e||r.__shopifyOriginTrialsDone){t();return}e.addEventListener("load",t,{once:!0}),e.addEventListener("error",t,{once:!0})}function i(){_(()=>r.setTimeout(f,0))}function m(){o.addEventListener("DOMContentLoaded",i,{once:!0})}p()?i():m()}s("\/\/www.goldbio.com\/cdn\/shopifycloud\/storefront\/assets\/storefront\/webmcp-c6b62ece.js","sha256-ke4DDuBjdvea3vxLWxHc9E9nq8zihuG4IfYEGbb77ys=");})();
699</script>
vendor: 1 bytes, line 699
699
700<script data-source-attribution="shopify.dynamic_checkout.dynamic.init">var Shopify=Shopify||{};Shopify.PaymentButton=Shopify.PaymentButton||{isStorefrontPortableWallets:!0,init:function(){window.Shopify.PaymentButton.init=function(){};var t=document.createElement("script");t.src="https://www.goldbio.com/cdn/shopifycloud/portable-wallets/latest/portable-wallets.en.js",t.type="module",document.head.appendChild(t)}};
701</script>
vendor: 1 bytes, line 701
701
702<script data-source-attribution="shopify.dynamic_checkout.buyer_consent">
703  function portableWalletsHideBuyerConsent(e){var t=document.getElementById("shopify-buyer-consent"),n=document.getElementById("shopify-subscription-policy-button");t&&n&&(t.classList.add("hidden"),t.setAttribute("aria-hidden","true"),n.removeEventListener("click",e))}function portableWalletsShowBuyerConsent(e){var t=document.getElementById("shopify-buyer-consent"),n=document.getElementById("shopify-subscription-policy-button");t&&n&&(t.classList.remove("hidden"),t.removeAttribute("aria-hidden"),n.addEventListener("click",e))}window.Shopify?.PaymentButton&&(window.Shopify.PaymentButton.hideBuyerConsent=portableWalletsHideBuyerConsent,window.Shopify.PaymentButton.showBuyerConsent=portableWalletsShowBuyerConsent);
704</script>
vendor: 1 bytes, line 704
704
705<script data-source-attribution="shopify.dynamic_checkout.cart.bootstrap">document.addEventListener("DOMContentLoaded",(function(){function t(){return document.querySelector("shopify-accelerated-checkout-cart, shopify-accelerated-checkout")}if(t())Shopify.PaymentButton.init();else{new MutationObserver((function(e,n){t()&&(Shopify.PaymentButton.init(),n.disconnect())})).observe(document.body,{childList:!0,subtree:!0})}}));
706</script>
vendor: 1 bytes, line 706
706
707<script id='scb4127' type='text/javascript' async='' src='https://www.goldbio.com/cdn/shopifycloud/privacy-banner/storefront-banner.js'></script>
vendor: 1 bytes, line 707
707
708<script id="shopify-cfh-end">window.performance && window.performance.mark && window.performance.mark('shopify.content_for_header.end');</script>
708
709    
710    <style data-shopify>
711      :root {
712      	--scheme-1:                      #ffffff;
713      	--scheme-1_solid:                #ffffff;
714      	--scheme-1_bg:                   var(--scheme-1);
715      	--scheme-1_bg_dark:              var(--scheme-1_bg);
716      	--scheme-1_bg_var:               var(--scheme-1_bg_dark);
717      	--scheme-1_bg_secondary:         #f4f4f4;
718      	--scheme-1_fg:                   #0b1613;
719      	--scheme-1_fg_sat:               #c0dfd7;
720      	--scheme-1_title:                #0071d0;
721      	--scheme-1_title_solid:          #0071d0;
722
723      	--scheme-1_primary_btn_bg:       #8ebf4d;
724      	--scheme-1_primary_btn_bg_dark:  #7cab3d;
725      	--scheme-1_primary_btn_fg:       #ffffff;
726      	--scheme-1_secondary_btn_bg:     #e6943d;
727      	--scheme-1_secondary_btn_bg_dark:#e2831d;
728      	--scheme-1_secondary_btn_fg:     #000000;
729      	--scheme-1_tertiary_btn_bg:      #0071d0;
730      	--scheme-1_tertiary_btn_bg_dark: #005eac;
731      	--scheme-1_tertiary_btn_fg:      #ffffff;
732      	--scheme-1_btn_bg:       		var(--scheme-1_primary_btn_bg);
733      	--scheme-1_btn_bg_dark:  		var(--scheme-1_primary_btn_bg_dark);
734      	--scheme-1_btn_fg:       		var(--scheme-1_primary_btn_fg);
735      	--scheme-1_bd:                   #e5e5e5;
736      	--scheme-1_input_bg:             #ffffff;
737      	--scheme-1_input_fg:             #0b1613;
738      	--scheme-1_input_pl:             #1c3830;
739      	--scheme-1_accent:               #f0702c;
740      	--scheme-1_accent_gradient:      linear-gradient(320deg, rgba(232, 74, 147, 1) 4%, rgba(239, 179, 76, 1) 100%);
741      	
742      	--scheme-1_fg_brightness: 0; 
743      	
744      		--primary_text_rgba:  				rgb(11, 22, 19);
745      		--body_bg:            				var(--scheme-1_bg);
746      	
747      	}select, .bv_atual, #root #content .f8sr select,
748      	[class^="palette-scheme-1"] select,
749      	[class^="palette-scheme-1"] .bv_atual,
750      	#root #content [class^="palette-scheme-1"] .f8sr select {
751      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
752      	}
753      	
754      		#root input[type="date"], #root .datepicker-input { background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 14 16' viewBox='0 0 14 16' fill='%230b1613'%3E%3Cpath d='M12.3 2H11V.8c0-.5-.3-.8-.7-.8s-.8.3-.8.8V2h-5V.8c0-.5-.3-.8-.7-.8S3 .3 3 .8V2H1.8C.8 2 0 2.8 0 3.8v10.5c0 1 .8 1.8 1.8 1.8h10.5c1 0 1.8-.8 1.8-1.8V3.8c-.1-1-.9-1.8-1.8-1.8zm.2 12.3c0 .1-.1.3-.3.3H1.8c-.1 0-.3-.1-.3-.3V7.5h11v6.8zm0-8.3h-11V3.8c0-.1.1-.3.3-.3h10.5c.1 0 .3.1.3.3V6z'/%3E%3C/svg%3E"); }
755      	
756      	select:focus, #root #content .f8sr select:focus,
757      	[class^="palette-scheme-1"] select:focus,
758      	#root #content [class^="palette-scheme-1"] .f8sr select:focus {
759      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
760      	}
761      	:root, [class^="palette-scheme-1"], [data-active-content*="scheme-1"] {
762      	--primary_bg:             var(--scheme-1_bg);
763      	--bg_secondary:           var(--scheme-1_bg_secondary);
764      	--primary_text:           var(--scheme-1_fg);
765      	--primary_text_h:         var(--scheme-1_title);
766      	--headings_text:          var(--primary_text_h);
767      	--headings_text_solid:    var(--scheme-1_title_solid);
768      	--primary_bg_btn:         var(--scheme-1_primary_btn_bg);
769      	--primary_bg_btn_dark:    var(--scheme-1_primary_btn_bg_dark);
770      	--primary_btn_text:       var(--scheme-1_primary_btn_fg);
771      	--secondary_bg_btn:       var(--scheme-1_secondary_btn_bg);
772      	--secondary_bg_btn_dark:  var(--scheme-1_secondary_btn_bg_dark);
773      	--secondary_btn_text:     var(--scheme-1_secondary_btn_fg);
774      	--tertiary_bg_btn:        var(--scheme-1_tertiary_btn_bg);
775      	--tertiary_bg_btn_dark:   var(--scheme-1_tertiary_btn_bg_dark);
776      	--tertiary_btn_text:      var(--scheme-1_tertiary_btn_fg);
777      	--custom_input_bg:        var(--scheme-1_input_bg);
778      	--custom_input_fg:        var(--scheme-1_input_fg);
779      	--custom_input_pl:        var(--scheme-1_input_pl);
780      	--custom_input_bd:        var(--scheme-1_bd);
781      	--custom_bd:              var(--custom_input_bd);
782      	--secondary_bg: 		  var(--primary_bg_btn);
783      	--accent:                 var(--scheme-1_accent);
784      	--accent_gradient:        var(--scheme-1_accent_gradient);
785      	--primary_text_brightness:var(--scheme-1_fg_brightness);
786      	}
787      	
788      :root {
789      	--scheme-2:                      linear-gradient(-90deg, rgba(106, 106, 106, 1), rgba(76, 76, 76, 1) 100%);
790      	--scheme-2_solid:                #0b1613;
791      	--scheme-2_bg:                   var(--scheme-2);
792      	--scheme-2_bg_dark:              #080f0d;
793      	--scheme-2_bg_var:               var(--scheme-2_bg_dark);
794      	--scheme-2_bg_secondary:         #8a8a9e;
795      	--scheme-2_fg:                   #bbbbbb;
796      	--scheme-2_fg_sat:               #ffffff;
797      	--scheme-2_title:                #ffffff;
798      	--scheme-2_title_solid:          #ffffff;
799
800      	--scheme-2_primary_btn_bg:       #ffffff;
801      	--scheme-2_primary_btn_bg_dark:  #ededed;
802      	--scheme-2_primary_btn_fg:       #0b1613;
803      	--scheme-2_secondary_btn_bg:     #fbea61;
804      	--scheme-2_secondary_btn_bg_dark:#fae53e;
805      	--scheme-2_secondary_btn_fg:     #000000;
806      	--scheme-2_tertiary_btn_bg:      #000096;
807      	--scheme-2_tertiary_btn_bg_dark: #000072;
808      	--scheme-2_tertiary_btn_fg:      #ffffff;
809      	--scheme-2_btn_bg:       		var(--scheme-2_primary_btn_bg);
810      	--scheme-2_btn_bg_dark:  		var(--scheme-2_primary_btn_bg_dark);
811      	--scheme-2_btn_fg:       		var(--scheme-2_primary_btn_fg);
812      	--scheme-2_bd:                   #fafafa;
813      	--scheme-2_input_bg:             #ffffff;
814      	--scheme-2_input_fg:             #0b1613;
815      	--scheme-2_input_pl:             #1c3830;
816      	--scheme-2_accent:               #fbea61;
817      	--scheme-2_accent_gradient:      #fbea61;
818      	
819      	--scheme-2_fg_brightness: 1; 
820      	
821      	}
822      	[class^="palette-scheme-2"] select,
823      	[class^="palette-scheme-2"] .bv_atual,
824      	#root #content [class^="palette-scheme-2"] .f8sr select {
825      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
825ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
826      	}
827      	
828      	
829      	[class^="palette-scheme-2"] select:focus,
830      	#root #content [class^="palette-scheme-2"] .f8sr select:focus {
831      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
832      	}
833      	[class^="palette-scheme-2"], [data-active-content*="scheme-2"] {
834      	--primary_bg:             var(--scheme-2_bg);
835      	--bg_secondary:           var(--scheme-2_bg_secondary);
836      	--primary_text:           var(--scheme-2_fg);
837      	--primary_text_h:         var(--scheme-2_title);
838      	--headings_text:          var(--primary_text_h);
839      	--headings_text_solid:    var(--scheme-2_title_solid);
840      	--primary_bg_btn:         var(--scheme-2_primary_btn_bg);
841      	--primary_bg_btn_dark:    var(--scheme-2_primary_btn_bg_dark);
842      	--primary_btn_text:       var(--scheme-2_primary_btn_fg);
843      	--secondary_bg_btn:       var(--scheme-2_secondary_btn_bg);
844      	--secondary_bg_btn_dark:  var(--scheme-2_secondary_btn_bg_dark);
845      	--secondary_btn_text:     var(--scheme-2_secondary_btn_fg);
846      	--tertiary_bg_btn:        var(--scheme-2_tertiary_btn_bg);
847      	--tertiary_bg_btn_dark:   var(--scheme-2_tertiary_btn_bg_dark);
848      	--tertiary_btn_text:      var(--scheme-2_tertiary_btn_fg);
849      	--custom_input_bg:        var(--scheme-2_input_bg);
850      	--custom_input_fg:        var(--scheme-2_input_fg);
851      	--custom_input_pl:        var(--scheme-2_input_pl);
852      	--custom_input_bd:        var(--scheme-2_bd);
853      	--custom_bd:              var(--custom_input_bd);
854      	--secondary_bg: 		  var(--primary_bg_btn);
855      	--accent:                 var(--scheme-2_accent);
856      	--accent_gradient:        var(--scheme-2_accent_gradient);
857      	--primary_text_brightness:var(--scheme-2_fg_brightness);
858      	}
859      	
860      :root {
861      	--scheme-3:                      #ffffff;
862      	--scheme-3_solid:                #ffffff;
863      	--scheme-3_bg:                   var(--scheme-3);
864      	--scheme-3_bg_dark:              var(--scheme-3_bg);
865      	--scheme-3_bg_var:               var(--scheme-3_bg_dark);
866      	--scheme-3_bg_secondary:         #ffffff;
867      	--scheme-3_fg:                   #4c4c4c;
868      	--scheme-3_fg_sat:               #ffffff;
869      	--scheme-3_title:                #0071d0;
870      	--scheme-3_title_solid:          #0071d0;
871
872      	--scheme-3_primary_btn_bg:       #e6943d;
873      	--scheme-3_primary_btn_bg_dark:  #e2831d;
874      	--scheme-3_primary_btn_fg:       #ffffff;
875      	--scheme-3_secondary_btn_bg:     #0b1613;
876      	--scheme-3_secondary_btn_bg_dark:#000000;
877      	--scheme-3_secondary_btn_fg:     #ffffff;
878      	--scheme-3_tertiary_btn_bg:      #0071d0;
879      	--scheme-3_tertiary_btn_bg_dark: #005eac;
880      	--scheme-3_tertiary_btn_fg:      #ffffff;
881      	--scheme-3_btn_bg:       		var(--scheme-3_primary_btn_bg);
882      	--scheme-3_btn_bg_dark:  		var(--scheme-3_primary_btn_bg_dark);
883      	--scheme-3_btn_fg:       		var(--scheme-3_primary_btn_fg);
884      	--scheme-3_bd:                   #dedcdc;
885      	--scheme-3_input_bg:             #ffffff;
886      	--scheme-3_input_fg:             #0b1613;
887      	--scheme-3_input_pl:             #1c3830;
888      	--scheme-3_accent:               #f0702c;
889      	--scheme-3_accent_gradient:      #f0702c;
890      	
891      	--scheme-3_fg_brightness: 0; 
892      	
893      	}
894      	[class^="palette-scheme-3"] select,
895      	[class^="palette-scheme-3"] .bv_atual,
896      	#root #content [class^="palette-scheme-3"] .f8sr select {
897      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
897ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
898      	}
899      	
900      	
901      	[class^="palette-scheme-3"] select:focus,
902      	#root #content [class^="palette-scheme-3"] .f8sr select:focus {
903      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
904      	}
905      	[class^="palette-scheme-3"], [data-active-content*="scheme-3"] {
906      	--primary_bg:             var(--scheme-3_bg);
907      	--bg_secondary:           var(--scheme-3_bg_secondary);
908      	--primary_text:           var(--scheme-3_fg);
909      	--primary_text_h:         var(--scheme-3_title);
910      	--headings_text:          var(--primary_text_h);
911      	--headings_text_solid:    var(--scheme-3_title_solid);
912      	--primary_bg_btn:         var(--scheme-3_primary_btn_bg);
913      	--primary_bg_btn_dark:    var(--scheme-3_primary_btn_bg_dark);
914      	--primary_btn_text:       var(--scheme-3_primary_btn_fg);
915      	--secondary_bg_btn:       var(--scheme-3_secondary_btn_bg);
916      	--secondary_bg_btn_dark:  var(--scheme-3_secondary_btn_bg_dark);
917      	--secondary_btn_text:     var(--scheme-3_secondary_btn_fg);
918      	--tertiary_bg_btn:        var(--scheme-3_tertiary_btn_bg);
919      	--tertiary_bg_btn_dark:   var(--scheme-3_tertiary_btn_bg_dark);
920      	--tertiary_btn_text:      var(--scheme-3_tertiary_btn_fg);
921      	--custom_input_bg:        var(--scheme-3_input_bg);
922      	--custom_input_fg:        var(--scheme-3_input_fg);
923      	--custom_input_pl:        var(--scheme-3_input_pl);
924      	--custom_input_bd:        var(--scheme-3_bd);
925      	--custom_bd:              var(--custom_input_bd);
926      	--secondary_bg: 		  var(--primary_bg_btn);
927      	--accent:                 var(--scheme-3_accent);
928      	--accent_gradient:        var(--scheme-3_accent_gradient);
929      	--primary_text_brightness:var(--scheme-3_fg_brightness);
930      	}
931      	
932      :root {
933      	--scheme-4:                      #0071d0;
934      	--scheme-4_solid:                #0071d0;
935      	--scheme-4_bg:                   var(--scheme-4);
936      	--scheme-4_bg_dark:              #006bc6;
937      	--scheme-4_bg_var:               var(--scheme-4_bg_dark);
938      	--scheme-4_bg_secondary:         #f4f4f4;
939      	--scheme-4_fg:                   #ffffff;
940      	--scheme-4_fg_sat:               #ffffff;
941      	--scheme-4_title:                #ffffff;
942      	--scheme-4_title_solid:          #ffffff;
943
944      	--scheme-4_primary_btn_bg:       #fbea61;
945      	--scheme-4_primary_btn_bg_dark:  #fae53e;
946      	--scheme-4_primary_btn_fg:       #000000;
947      	--scheme-4_secondary_btn_bg:     #ffffff;
948      	--scheme-4_secondary_btn_bg_dark:#ededed;
949      	--scheme-4_secondary_btn_fg:     #0b1613;
950      	--scheme-4_tertiary_btn_bg:      #ffffff;
951      	--scheme-4_tertiary_btn_bg_dark: #ededed;
952      	--scheme-4_tertiary_btn_fg:      #0b1613;
953      	--scheme-4_btn_bg:       		var(--scheme-4_primary_btn_bg);
954      	--scheme-4_btn_bg_dark:  		var(--scheme-4_primary_btn_bg_dark);
955      	--scheme-4_btn_fg:       		var(--scheme-4_primary_btn_fg);
956      	--scheme-4_bd:                   #fafafa;
957      	--scheme-4_input_bg:             #ffffff;
958      	--scheme-4_input_fg:             #0b1613;
959      	--scheme-4_input_pl:             #1c3830;
960      	--scheme-4_accent:               #f0702c;
961      	--scheme-4_accent_gradient:      #f0702c;
962      	
963      	--scheme-4_fg_brightness: 1; 
964      	
965      	}
966      	[class^="palette-scheme-4"] select,
967      	[class^="palette-scheme-4"] .bv_atual,
968      	#root #content [class^="palette-scheme-4"] .f8sr select {
969      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
969ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
970      	}
971      	
972      	
973      	[class^="palette-scheme-4"] select:focus,
974      	#root #content [class^="palette-scheme-4"] .f8sr select:focus {
975      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
976      	}
977      	[class^="palette-scheme-4"], [data-active-content*="scheme-4"] {
978      	--primary_bg:             var(--scheme-4_bg);
979      	--bg_secondary:           var(--scheme-4_bg_secondary);
980      	--primary_text:           var(--scheme-4_fg);
981      	--primary_text_h:         var(--scheme-4_title);
982      	--headings_text:          var(--primary_text_h);
983      	--headings_text_solid:    var(--scheme-4_title_solid);
984      	--primary_bg_btn:         var(--scheme-4_primary_btn_bg);
985      	--primary_bg_btn_dark:    var(--scheme-4_primary_btn_bg_dark);
986      	--primary_btn_text:       var(--scheme-4_primary_btn_fg);
987      	--secondary_bg_btn:       var(--scheme-4_secondary_btn_bg);
988      	--secondary_bg_btn_dark:  var(--scheme-4_secondary_btn_bg_dark);
989      	--secondary_btn_text:     var(--scheme-4_secondary_btn_fg);
990      	--tertiary_bg_btn:        var(--scheme-4_tertiary_btn_bg);
991      	--tertiary_bg_btn_dark:   var(--scheme-4_tertiary_btn_bg_dark);
992      	--tertiary_btn_text:      var(--scheme-4_tertiary_btn_fg);
993      	--custom_input_bg:        var(--scheme-4_input_bg);
994      	--custom_input_fg:        var(--scheme-4_input_fg);
995      	--custom_input_pl:        var(--scheme-4_input_pl);
996      	--custom_input_bd:        var(--scheme-4_bd);
997      	--custom_bd:              var(--custom_input_bd);
998      	--secondary_bg: 		  var(--primary_bg_btn);
999      	--accent:                 var(--scheme-4_accent);
1000      	--accent_gradient:        var(--scheme-4_accent_gradient);
1001      	--primary_text_brightness:var(--scheme-4_fg_brightness);
1002      	}
1003      	
1004      :root {
1005      	--scheme-5:                      #f0702c;
1006      	--scheme-5_solid:                #f0702c;
1007      	--scheme-5_bg:                   var(--scheme-5);
1008      	--scheme-5_bg_dark:              #ef6a22;
1009      	--scheme-5_bg_var:               var(--scheme-5_bg_dark);
1010      	--scheme-5_bg_secondary:         #ffffff;
1011      	--scheme-5_fg:                   #ffffff;
1012      	--scheme-5_fg_sat:               #ffffff;
1013      	--scheme-5_title:                #ffffff;
1014      	--scheme-5_title_solid:          #ffffff;
1015
1016      	--scheme-5_primary_btn_bg:       #ffffff;
1017      	--scheme-5_primary_btn_bg_dark:  #ededed;
1018      	--scheme-5_primary_btn_fg:       #0b1613;
1019      	--scheme-5_secondary_btn_bg:     #0b1613;
1020      	--scheme-5_secondary_btn_bg_dark:#000000;
1021      	--scheme-5_secondary_btn_fg:     #ffffff;
1022      	--scheme-5_tertiary_btn_bg:      #0071d0;
1023      	--scheme-5_tertiary_btn_bg_dark: #005eac;
1024      	--scheme-5_tertiary_btn_fg:      #ffffff;
1025      	--scheme-5_btn_bg:       		var(--scheme-5_primary_btn_bg);
1026      	--scheme-5_btn_bg_dark:  		var(--scheme-5_primary_btn_bg_dark);
1027      	--scheme-5_btn_fg:       		var(--scheme-5_primary_btn_fg);
1028      	--scheme-5_bd:                   #fafafa;
1029      	--scheme-5_input_bg:             #ffffff;
1030      	--scheme-5_input_fg:             #0b1613;
1031      	--scheme-5_input_pl:             #1c3830;
1032      	--scheme-5_accent:               #ffffff;
1033      	--scheme-5_accent_gradient:      #ffffff;
1034      	
1035      	--scheme-5_fg_brightness: 1; 
1036      	
1037      	}
1038      	[class^="palette-scheme-5"] select,
1039      	[class^="palette-scheme-5"] .bv_atual,
1040      	#root #content [class^="palette-scheme-5"] .f8sr select {
1041      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
1041ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
1042      	}
1043      	
1044      	
1045      	[class^="palette-scheme-5"] select:focus,
1046      	#root #content [class^="palette-scheme-5"] .f8sr select:focus {
1047      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
1048      	}
1049      	[class^="palette-scheme-5"], [data-active-content*="scheme-5"] {
1050      	--primary_bg:             var(--scheme-5_bg);
1051      	--bg_secondary:           var(--scheme-5_bg_secondary);
1052      	--primary_text:           var(--scheme-5_fg);
1053      	--primary_text_h:         var(--scheme-5_title);
1054      	--headings_text:          var(--primary_text_h);
1055      	--headings_text_solid:    var(--scheme-5_title_solid);
1056      	--primary_bg_btn:         var(--scheme-5_primary_btn_bg);
1057      	--primary_bg_btn_dark:    var(--scheme-5_primary_btn_bg_dark);
1058      	--primary_btn_text:       var(--scheme-5_primary_btn_fg);
1059      	--secondary_bg_btn:       var(--scheme-5_secondary_btn_bg);
1060      	--secondary_bg_btn_dark:  var(--scheme-5_secondary_btn_bg_dark);
1061      	--secondary_btn_text:     var(--scheme-5_secondary_btn_fg);
1062      	--tertiary_bg_btn:        var(--scheme-5_tertiary_btn_bg);
1063      	--tertiary_bg_btn_dark:   var(--scheme-5_tertiary_btn_bg_dark);
1064      	--tertiary_btn_text:      var(--scheme-5_tertiary_btn_fg);
1065      	--custom_input_bg:        var(--scheme-5_input_bg);
1066      	--custom_input_fg:        var(--scheme-5_input_fg);
1067      	--custom_input_pl:        var(--scheme-5_input_pl);
1068      	--custom_input_bd:        var(--scheme-5_bd);
1069      	--custom_bd:              var(--custom_input_bd);
1070      	--secondary_bg: 		  var(--primary_bg_btn);
1071      	--accent:                 var(--scheme-5_accent);
1072      	--accent_gradient:        var(--scheme-5_accent_gradient);
1073      	--primary_text_brightness:var(--scheme-5_fg_brightness);
1074      	}
1075      	
1076      :root {
1077      	--scheme-6:                      linear-gradient(127deg, rgba(242, 247, 249, 1) 11%, rgba(233, 241, 244, 1) 81%);
1078      	--scheme-6_solid:                #f4f4f4;
1079      	--scheme-6_bg:                   var(--scheme-6);
1080      	--scheme-6_bg_dark:              #efefef;
1081      	--scheme-6_bg_var:               var(--scheme-6_bg_dark);
1082      	--scheme-6_bg_secondary:         #ffffff;
1083      	--scheme-6_fg:                   #0b1613;
1084      	--scheme-6_fg_sat:               #c0dfd7;
1085      	--scheme-6_title:                #0b1613;
1086      	--scheme-6_title_solid:          #0b1613;
1087
1088      	--scheme-6_primary_btn_bg:       #ffffff;
1089      	--scheme-6_primary_btn_bg_dark:  #ededed;
1090      	--scheme-6_primary_btn_fg:       #000000;
1091      	--scheme-6_secondary_btn_bg:     #fbea61;
1092      	--scheme-6_secondary_btn_bg_dark:#fae53e;
1093      	--scheme-6_secondary_btn_fg:     #000000;
1094      	--scheme-6_tertiary_btn_bg:      #0b1613;
1095      	--scheme-6_tertiary_btn_bg_dark: #000000;
1096      	--scheme-6_tertiary_btn_fg:      #ffffff;
1097      	--scheme-6_btn_bg:       		var(--scheme-6_primary_btn_bg);
1098      	--scheme-6_btn_bg_dark:  		var(--scheme-6_primary_btn_bg_dark);
1099      	--scheme-6_btn_fg:       		var(--scheme-6_primary_btn_fg);
1100      	--scheme-6_bd:                   #fafafa;
1101      	--scheme-6_input_bg:             #ffffff;
1102      	--scheme-6_input_fg:             #0b1613;
1103      	--scheme-6_input_pl:             #1c3830;
1104      	--scheme-6_accent:               #0071d0;
1105      	--scheme-6_accent_gradient:      #0071d0;
1106      	
1107      	--scheme-6_fg_brightness: 0; 
1108      	
1109      	}
1110      	[class^="palette-scheme-6"] select,
1111      	[class^="palette-scheme-6"] .bv_atual,
1112      	#root #content [class^="palette-scheme-6"] .f8sr select {
1113      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
1113ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
1114      	}
1115      	
1116      	
1117      	[class^="palette-scheme-6"] select:focus,
1118      	#root #content [class^="palette-scheme-6"] .f8sr select:focus {
1119      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
1120      	}
1121      	[class^="palette-scheme-6"], [data-active-content*="scheme-6"] {
1122      	--primary_bg:             var(--scheme-6_bg);
1123      	--bg_secondary:           var(--scheme-6_bg_secondary);
1124      	--primary_text:           var(--scheme-6_fg);
1125      	--primary_text_h:         var(--scheme-6_title);
1126      	--headings_text:          var(--primary_text_h);
1127      	--headings_text_solid:    var(--scheme-6_title_solid);
1128      	--primary_bg_btn:         var(--scheme-6_primary_btn_bg);
1129      	--primary_bg_btn_dark:    var(--scheme-6_primary_btn_bg_dark);
1130      	--primary_btn_text:       var(--scheme-6_primary_btn_fg);
1131      	--secondary_bg_btn:       var(--scheme-6_secondary_btn_bg);
1132      	--secondary_bg_btn_dark:  var(--scheme-6_secondary_btn_bg_dark);
1133      	--secondary_btn_text:     var(--scheme-6_secondary_btn_fg);
1134      	--tertiary_bg_btn:        var(--scheme-6_tertiary_btn_bg);
1135      	--tertiary_bg_btn_dark:   var(--scheme-6_tertiary_btn_bg_dark);
1136      	--tertiary_btn_text:      var(--scheme-6_tertiary_btn_fg);
1137      	--custom_input_bg:        var(--scheme-6_input_bg);
1138      	--custom_input_fg:        var(--scheme-6_input_fg);
1139      	--custom_input_pl:        var(--scheme-6_input_pl);
1140      	--custom_input_bd:        var(--scheme-6_bd);
1141      	--custom_bd:              var(--custom_input_bd);
1142      	--secondary_bg: 		  var(--primary_bg_btn);
1143      	--accent:                 var(--scheme-6_accent);
1144      	--accent_gradient:        var(--scheme-6_accent_gradient);
1145      	--primary_text_brightness:var(--scheme-6_fg_brightness);
1146      	}
1147      	
1148      :root {
1149      	--scheme-7:                      #d5f1ee;
1150      	--scheme-7_solid:                #d5f1ee;
1151      	--scheme-7_bg:                   var(--scheme-7);
1152      	--scheme-7_bg_dark:              #cdeeeb;
1153      	--scheme-7_bg_var:               var(--scheme-7_bg_dark);
1154      	--scheme-7_bg_secondary:         #f0702c;
1155      	--scheme-7_fg:                   #0b1613;
1156      	--scheme-7_fg_sat:               #c0dfd7;
1157      	--scheme-7_title:                #0b1613;
1158      	--scheme-7_title_solid:          #0b1613;
1159
1160      	--scheme-7_primary_btn_bg:       #0071d0;
1161      	--scheme-7_primary_btn_bg_dark:  #005eac;
1162      	--scheme-7_primary_btn_fg:       #ffffff;
1163      	--scheme-7_secondary_btn_bg:     #fbea61;
1164      	--scheme-7_secondary_btn_bg_dark:#fae53e;
1165      	--scheme-7_secondary_btn_fg:     #0b1613;
1166      	--scheme-7_tertiary_btn_bg:      #0071d0;
1167      	--scheme-7_tertiary_btn_bg_dark: #005eac;
1168      	--scheme-7_tertiary_btn_fg:      #ffffff;
1169      	--scheme-7_btn_bg:       		var(--scheme-7_primary_btn_bg);
1170      	--scheme-7_btn_bg_dark:  		var(--scheme-7_primary_btn_bg_dark);
1171      	--scheme-7_btn_fg:       		var(--scheme-7_primary_btn_fg);
1172      	--scheme-7_bd:                   #e5e5e5;
1173      	--scheme-7_input_bg:             #ffffff;
1174      	--scheme-7_input_fg:             #0b1613;
1175      	--scheme-7_input_pl:             #1c3830;
1176      	--scheme-7_accent:               #0071d0;
1177      	--scheme-7_accent_gradient:      #0071d0;
1178      	
1179      	--scheme-7_fg_brightness: 0; 
1180      	
1181      	}
1182      	[class^="palette-scheme-7"] select,
1183      	[class^="palette-scheme-7"] .bv_atual,
1184      	#root #content [class^="palette-scheme-7"] .f8sr select {
1185      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
1185ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
1186      	}
1187      	
1188      	
1189      	[class^="palette-scheme-7"] select:focus,
1190      	#root #content [class^="palette-scheme-7"] .f8sr select:focus {
1191      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
1192      	}
1193      	[class^="palette-scheme-7"], [data-active-content*="scheme-7"] {
1194      	--primary_bg:             var(--scheme-7_bg);
1195      	--bg_secondary:           var(--scheme-7_bg_secondary);
1196      	--primary_text:           var(--scheme-7_fg);
1197      	--primary_text_h:         var(--scheme-7_title);
1198      	--headings_text:          var(--primary_text_h);
1199      	--headings_text_solid:    var(--scheme-7_title_solid);
1200      	--primary_bg_btn:         var(--scheme-7_primary_btn_bg);
1201      	--primary_bg_btn_dark:    var(--scheme-7_primary_btn_bg_dark);
1202      	--primary_btn_text:       var(--scheme-7_primary_btn_fg);
1203      	--secondary_bg_btn:       var(--scheme-7_secondary_btn_bg);
1204      	--secondary_bg_btn_dark:  var(--scheme-7_secondary_btn_bg_dark);
1205      	--secondary_btn_text:     var(--scheme-7_secondary_btn_fg);
1206      	--tertiary_bg_btn:        var(--scheme-7_tertiary_btn_bg);
1207      	--tertiary_bg_btn_dark:   var(--scheme-7_tertiary_btn_bg_dark);
1208      	--tertiary_btn_text:      var(--scheme-7_tertiary_btn_fg);
1209      	--custom_input_bg:        var(--scheme-7_input_bg);
1210      	--custom_input_fg:        var(--scheme-7_input_fg);
1211      	--custom_input_pl:        var(--scheme-7_input_pl);
1212      	--custom_input_bd:        var(--scheme-7_bd);
1213      	--custom_bd:              var(--custom_input_bd);
1214      	--secondary_bg: 		  var(--primary_bg_btn);
1215      	--accent:                 var(--scheme-7_accent);
1216      	--accent_gradient:        var(--scheme-7_accent_gradient);
1217      	--primary_text_brightness:var(--scheme-7_fg_brightness);
1218      	}
1219      	
1220      :root {
1221      	--scheme-8:                      linear-gradient(320deg, rgba(232, 74, 147, 1) 4%, rgba(239, 179, 76, 1) 100%);
1222      	--scheme-8_solid:                #f0702c;
1223      	--scheme-8_bg:                   var(--scheme-8);
1224      	--scheme-8_bg_dark:              #ef6a22;
1225      	--scheme-8_bg_var:               var(--scheme-8_bg_dark);
1226      	--scheme-8_bg_secondary:         #f4f4f4;
1227      	--scheme-8_fg:                   #ffffff;
1228      	--scheme-8_fg_sat:               #ffffff;
1229      	--scheme-8_title:                #ffffff;
1230      	--scheme-8_title_solid:          #ffffff;
1231
1232      	--scheme-8_primary_btn_bg:       #f0702c;
1233      	--scheme-8_primary_btn_bg_dark:  #e85b10;
1234      	--scheme-8_primary_btn_fg:       #ffffff;
1235      	--scheme-8_secondary_btn_bg:     #ffffff;
1236      	--scheme-8_secondary_btn_bg_dark:#ededed;
1237      	--scheme-8_secondary_btn_fg:     #0b1613;
1238      	--scheme-8_tertiary_btn_bg:      #0071d0;
1239      	--scheme-8_tertiary_btn_bg_dark: #005eac;
1240      	--scheme-8_tertiary_btn_fg:      #ffffff;
1241      	--scheme-8_btn_bg:       		var(--scheme-8_primary_btn_bg);
1242      	--scheme-8_btn_bg_dark:  		var(--scheme-8_primary_btn_bg_dark);
1243      	--scheme-8_btn_fg:       		var(--scheme-8_primary_btn_fg);
1244      	--scheme-8_bd:                   #fafafa;
1245      	--scheme-8_input_bg:             #ffffff;
1246      	--scheme-8_input_fg:             #0b1613;
1247      	--scheme-8_input_pl:             #1c3830;
1248      	--scheme-8_accent:               #f0702c;
1249      	--scheme-8_accent_gradient:      #f0702c;
1250      	
1251      	--scheme-8_fg_brightness: 1; 
1252      	
1253      	}
1254      	[class^="palette-scheme-8"] select,
1255      	[class^="palette-scheme-8"] .bv_atual,
1256      	#root #content [class^="palette-scheme-8"] .f8sr select {
1257      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
1257ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
1258      	}
1259      	
1260      	
1261      	[class^="palette-scheme-8"] select:focus,
1262      	#root #content [class^="palette-scheme-8"] .f8sr select:focus {
1263      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
1264      	}
1265      	[class^="palette-scheme-8"], [data-active-content*="scheme-8"] {
1266      	--primary_bg:             var(--scheme-8_bg);
1267      	--bg_secondary:           var(--scheme-8_bg_secondary);
1268      	--primary_text:           var(--scheme-8_fg);
1269      	--primary_text_h:         var(--scheme-8_title);
1270      	--headings_text:          var(--primary_text_h);
1271      	--headings_text_solid:    var(--scheme-8_title_solid);
1272      	--primary_bg_btn:         var(--scheme-8_primary_btn_bg);
1273      	--primary_bg_btn_dark:    var(--scheme-8_primary_btn_bg_dark);
1274      	--primary_btn_text:       var(--scheme-8_primary_btn_fg);
1275      	--secondary_bg_btn:       var(--scheme-8_secondary_btn_bg);
1276      	--secondary_bg_btn_dark:  var(--scheme-8_secondary_btn_bg_dark);
1277      	--secondary_btn_text:     var(--scheme-8_secondary_btn_fg);
1278      	--tertiary_bg_btn:        var(--scheme-8_tertiary_btn_bg);
1279      	--tertiary_bg_btn_dark:   var(--scheme-8_tertiary_btn_bg_dark);
1280      	--tertiary_btn_text:      var(--scheme-8_tertiary_btn_fg);
1281      	--custom_input_bg:        var(--scheme-8_input_bg);
1282      	--custom_input_fg:        var(--scheme-8_input_fg);
1283      	--custom_input_pl:        var(--scheme-8_input_pl);
1284      	--custom_input_bd:        var(--scheme-8_bd);
1285      	--custom_bd:              var(--custom_input_bd);
1286      	--secondary_bg: 		  var(--primary_bg_btn);
1287      	--accent:                 var(--scheme-8_accent);
1288      	--accent_gradient:        var(--scheme-8_accent_gradient);
1289      	--primary_text_brightness:var(--scheme-8_fg_brightness);
1290      	}
1291      	
1292      :root {
1293      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067:                      linear-gradient(-90deg, rgba(142, 191, 77, 1), rgba(97, 157, 28, 1) 100%);
1294      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_solid:                #ffffff;
1295      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg:                   var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067);
1296      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg_dark:              var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg);
1297      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg_var:               var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg_dark);
1298      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg_secondary:         #f4f4f4;
1299      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_fg:                   #ffffff;
1300      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_fg_sat:               #ffffff;
1301      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_title:                #ffffff;
1302      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_title_solid:          #ffffff;
1303
1304      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg:       #ffffff;
1305      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg_dark:  #ededed;
1306      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_fg:       #e6943d;
1307      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_secondary_btn_bg:     #e6943d;
1308      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_secondary_btn_bg_dark:#e2831d;
1309      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_secondary_btn_fg:     #000000;
1310      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_tertiary_btn_bg:      #e6943d;
1311      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_tertiary_btn_bg_dark: #e2831d;
1312      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_tertiary_btn_fg:      #ffffff;
1313      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_btn_bg:       		var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg);
1314      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_btn_bg_dark:  		var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg_dark);
1315      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_btn_fg:       		var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_fg);
1316      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bd:                   #ecedec;
1317      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_bg:             #ffffff;
1318      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_fg:             #0b1613;
1319      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_pl:             #1c3830;
1320      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_accent:               #f0702c;
1321      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_accent_gradient:      linear-gradient(320deg, rgba(232, 74, 147, 1) 4%, rgba(239, 179, 76, 1) 100%);
1322      	
1323      	--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_fg_brightness: 1; 
1324      	
1325      	}
1326      	[class^="palette-scheme-f84d67c2-3148-40bf-b6db-e141fd84f067"] select,
1327      	[class^="palette-scheme-f84d67c2-3148-40bf-b6db-e141fd84f067"] .bv_atual,
1328      	#root #content [class^="palette-scheme-f84d67c2-3148-40bf-b6db-e141fd84f067"] .f8sr select {
1329      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
1329ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
1330      	}
1331      	
1332      	
1333      	[class^="palette-scheme-f84d67c2-3148-40bf-b6db-e141fd84f067"] select:focus,
1334      	#root #content [class^="palette-scheme-f84d67c2-3148-40bf-b6db-e141fd84f067"] .f8sr select:focus {
1335      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
1336      	}
1337      	[class^="palette-scheme-f84d67c2-3148-40bf-b6db-e141fd84f067"], [data-active-content*="scheme-f84d67c2-3148-40bf-b6db-e141fd84f067"] {
1338      	--primary_bg:             var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg);
1339      	--bg_secondary:           var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg_secondary);
1340      	--primary_text:           var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_fg);
1341      	--primary_text_h:         var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_title);
1342      	--headings_text:          var(--primary_text_h);
1343      	--headings_text_solid:    var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_title_solid);
1344      	--primary_bg_btn:         var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg);
1345      	--primary_bg_btn_dark:    var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg_dark);
1346      	--primary_btn_text:       var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_fg);
1347      	--secondary_bg_btn:       var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_secondary_btn_bg);
1348      	--secondary_bg_btn_dark:  var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_secondary_btn_bg_dark);
1349      	--secondary_btn_text:     var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_secondary_btn_fg);
1350      	--tertiary_bg_btn:        var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_tertiary_btn_bg);
1351      	--tertiary_bg_btn_dark:   var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_tertiary_btn_bg_dark);
1352      	--tertiary_btn_text:      var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_tertiary_btn_fg);
1353      	--custom_input_bg:        var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_bg);
1354      	--custom_input_fg:        var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_fg);
1355      	--custom_input_pl:        var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_pl);
1356      	--custom_input_bd:        var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bd);
1357      	--custom_bd:              var(--custom_input_bd);
1358      	--secondary_bg: 		  var(--primary_bg_btn);
1359      	--accent:                 var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_accent);
1360      	--accent_gradient:        var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_accent_gradient);
1361      	--primary_text_brightness:var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_fg_brightness);
1362      	}
1363      	
1364      :root {
1365      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d:                      #4c4c4c;
1366      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_solid:                #4c4c4c;
1367      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bg:                   var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d);
1368      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bg_dark:              #474747;
1369      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bg_var:               var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bg_dark);
1370      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bg_secondary:         #f4f4f4;
1371      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_fg:                   #ffffff;
1372      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_fg_sat:               #ffffff;
1373      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_title:                #ffffff;
1374      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_title_solid:          #ffffff;
1375
1376      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_bg:       #bbbbbb;
1377      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_bg_dark:  #a9a9a9;
1378      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_fg:       #ffffff;
1379      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_secondary_btn_bg:     #e6943d;
1380      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_secondary_btn_bg_dark:#e2831d;
1381      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_secondary_btn_fg:     #000000;
1382      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_tertiary_btn_bg:      #0071d0;
1383      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_tertiary_btn_bg_dark: #005eac;
1384      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_tertiary_btn_fg:      #ffffff;
1385      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_btn_bg:       		var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_bg);
1386      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_btn_bg_dark:  		var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_bg_dark);
1387      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_btn_fg:       		var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_fg);
1388      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bd:                   #ecedec;
1389      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_input_bg:             #ffffff;
1390      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_input_fg:             #0b1613;
1391      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_input_pl:             #1c3830;
1392      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_accent:               #f0702c;
1393      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_accent_gradient:      linear-gradient(320deg, rgba(232, 74, 147, 1) 4%, rgba(239, 179, 76, 1) 100%);
1394      	
1395      	--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_fg_brightness: 1; 
1396      	
1397      	}
1398      	[class^="palette-scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d"] select,
1399      	[class^="palette-scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d"] .bv_atual,
1400      	#root #content [class^="palette-scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d"] .f8sr select {
1401      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
1401ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
1402      	}
1403      	
1404      	
1405      	[class^="palette-scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d"] select:focus,
1406      	#root #content [class^="palette-scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d"] .f8sr select:focus {
1407      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
1408      	}
1409      	[class^="palette-scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d"], [data-active-content*="scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d"] {
1410      	--primary_bg:             var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bg);
1411      	--bg_secondary:           var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bg_secondary);
1412      	--primary_text:           var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_fg);
1413      	--primary_text_h:         var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_title);
1414      	--headings_text:          var(--primary_text_h);
1415      	--headings_text_solid:    var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_title_solid);
1416      	--primary_bg_btn:         var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_bg);
1417      	--primary_bg_btn_dark:    var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_bg_dark);
1418      	--primary_btn_text:       var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_primary_btn_fg);
1419      	--secondary_bg_btn:       var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_secondary_btn_bg);
1420      	--secondary_bg_btn_dark:  var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_secondary_btn_bg_dark);
1421      	--secondary_btn_text:     var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_secondary_btn_fg);
1422      	--tertiary_bg_btn:        var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_tertiary_btn_bg);
1423      	--tertiary_bg_btn_dark:   var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_tertiary_btn_bg_dark);
1424      	--tertiary_btn_text:      var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_tertiary_btn_fg);
1425      	--custom_input_bg:        var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_input_bg);
1426      	--custom_input_fg:        var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_input_fg);
1427      	--custom_input_pl:        var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_input_pl);
1428      	--custom_input_bd:        var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bd);
1429      	--custom_bd:              var(--custom_input_bd);
1430      	--secondary_bg: 		  var(--primary_bg_btn);
1431      	--accent:                 var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_accent);
1432      	--accent_gradient:        var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_accent_gradient);
1433      	--primary_text_brightness:var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_fg_brightness);
1434      	}
1435      	
1436      :root {
1437      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd:                      #f4f2f2;
1438      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_solid:                #f4f2f2;
1439      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bg:                   var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd);
1440      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bg_dark:              #efecec;
1441      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bg_var:               var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bg_dark);
1442      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bg_secondary:         #f4f4f4;
1443      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_fg:                   #0b1613;
1444      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_fg_sat:               #c0dfd7;
1445      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_title:                #42565e;
1446      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_title_solid:          #42565e;
1447
1448      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_bg:       #0071d0;
1449      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_bg_dark:  #005eac;
1450      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_fg:       #ffffff;
1451      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_secondary_btn_bg:     #e6943d;
1452      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_secondary_btn_bg_dark:#e2831d;
1453      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_secondary_btn_fg:     #000000;
1454      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_tertiary_btn_bg:      #0071d0;
1455      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_tertiary_btn_bg_dark: #005eac;
1456      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_tertiary_btn_fg:      #ffffff;
1457      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_btn_bg:       		var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_bg);
1458      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_btn_bg_dark:  		var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_bg_dark);
1459      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_btn_fg:       		var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_fg);
1460      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bd:                   #ecedec;
1461      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_input_bg:             #ffffff;
1462      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_input_fg:             #0b1613;
1463      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_input_pl:             #1c3830;
1464      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_accent:               #f0702c;
1465      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_accent_gradient:      linear-gradient(320deg, rgba(232, 74, 147, 1) 4%, rgba(239, 179, 76, 1) 100%);
1466      	
1467      	--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_fg_brightness: 0; 
1468      	
1469      	}
1470      	[class^="palette-scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd"] select,
1471      	[class^="palette-scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd"] .bv_atual,
1472      	#root #content [class^="palette-scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd"] .f8sr select {
1473      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
1474      	}
1475      	
1476      	
1477      	[class^="palette-scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd"] select:focus,
1478      	#root #content [class^="palette-scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd"] .f8sr select:focus {
1479      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
1480      	}
1481      	[class^="palette-scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd"], [data-active-content*="scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd"] {
1482      	--primary_bg:             var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bg);
1483      	--bg_secondary:           var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bg_secondary);
1484      	--primary_text:           var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_fg);
1485      	--primary_text_h:         var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_title);
1486      	--headings_text:          var(--primary_text_h);
1487      	--headings_text_solid:    var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_title_solid);
1488      	--primary_bg_btn:         var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_bg);
1489      	--primary_bg_btn_dark:    var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_bg_dark);
1490      	--primary_btn_text:       var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_primary_btn_fg);
1491      	--secondary_bg_btn:       var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_secondary_btn_bg);
1492      	--secondary_bg_btn_dark:  var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_secondary_btn_bg_dark);
1493      	--secondary_btn_text:     var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_secondary_btn_fg);
1494      	--tertiary_bg_btn:        var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_tertiary_btn_bg);
1495      	--tertiary_bg_btn_dark:   var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_tertiary_btn_bg_dark);
1496      	--tertiary_btn_text:      var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_tert
1496iary_btn_fg);
1497      	--custom_input_bg:        var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_input_bg);
1498      	--custom_input_fg:        var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_input_fg);
1499      	--custom_input_pl:        var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_input_pl);
1500      	--custom_input_bd:        var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_bd);
1501      	--custom_bd:              var(--custom_input_bd);
1502      	--secondary_bg: 		  var(--primary_bg_btn);
1503      	--accent:                 var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_accent);
1504      	--accent_gradient:        var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_accent_gradient);
1505      	--primary_text_brightness:var(--scheme-314b065c-7afb-46c6-88bb-70ce5471e4bd_fg_brightness);
1506      	}
1507      	
1508      :root {
1509      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61:                      #f4f2f2;
1510      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_solid:                #f4f2f2;
1511      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bg:                   var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61);
1512      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bg_dark:              #efecec;
1513      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bg_var:               var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bg_dark);
1514      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bg_secondary:         #f4f4f4;
1515      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_fg:                   #0b1613;
1516      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_fg_sat:               #c0dfd7;
1517      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_title:                #0071d0;
1518      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_title_solid:          #0071d0;
1519
1520      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_bg:       #0071d0;
1521      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_bg_dark:  #005eac;
1522      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_fg:       #ffffff;
1523      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_secondary_btn_bg:     #e6943d;
1524      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_secondary_btn_bg_dark:#e2831d;
1525      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_secondary_btn_fg:     #000000;
1526      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_tertiary_btn_bg:      #0071d0;
1527      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_tertiary_btn_bg_dark: #005eac;
1528      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_tertiary_btn_fg:      #ffffff;
1529      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_btn_bg:       		var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_bg);
1530      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_btn_bg_dark:  		var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_bg_dark);
1531      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_btn_fg:       		var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_fg);
1532      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bd:                   #ecedec;
1533      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_input_bg:             #ffffff;
1534      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_input_fg:             #0b1613;
1535      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_input_pl:             #1c3830;
1536      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_accent:               #f0702c;
1537      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_accent_gradient:      linear-gradient(320deg, rgba(232, 74, 147, 1) 4%, rgba(239, 179, 76, 1) 100%);
1538      	
1539      	--scheme-c800998b-c7f1-4f24-82e6-971865496e61_fg_brightness: 0; 
1540      	
1541      	}
1542      	[class^="palette-scheme-c800998b-c7f1-4f24-82e6-971865496e61"] select,
1543      	[class^="palette-scheme-c800998b-c7f1-4f24-82e6-971865496e61"] .bv_atual,
1544      	#root #content [class^="palette-scheme-c800998b-c7f1-4f24-82e6-971865496e61"] .f8sr select {
1545      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewBox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M.3.2c.4-.3.9-.3 1.2.1l4.7 5.3 5-5.3c.3-.3.9-.4 1.2 0 .3.3.4.9 0 1.2l-5.7 6c-.1.1-.3.2-.5.2s-.5-.1-.6-.3l-5.3-6C-.1 1.1-.1.5.3.2z'/%3E%3C/svg%3E");
1546      	}
1547      	
1548      	
1549      	[class^="palette-scheme-c800998b-c7f1-4f24-82e6-971865496e61"] select:focus,
1550      	#root #content [class^="palette-scheme-c800998b-c7f1-4f24-82e6-971865496e61"] .f8sr select:focus {
1551      	background-image: url("data:image/svg+xml,%3Csvg xmlns='http://www.w3.org/2000/svg' xml:space='preserve' style='enable-background:new 0 0 12.7 7.7' viewB
1551ox='0 0 12.7 7.7' fill='%230b1613'%3E%3Cpath d='M12.4 7.5c-.4.3-.9.3-1.2-.1L6.5 2.1l-5 5.3c-.3.3-.9.4-1.2 0-.3-.3-.4-.9 0-1.2L6 .2c.1-.1.3-.2.5-.2.3 0 .5.1.6.3l5.3 6c.4.3.4.9 0 1.2z'/%3E%3C/svg%3E");
1552      	}
1553      	[class^="palette-scheme-c800998b-c7f1-4f24-82e6-971865496e61"], [data-active-content*="scheme-c800998b-c7f1-4f24-82e6-971865496e61"] {
1554      	--primary_bg:             var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bg);
1555      	--bg_secondary:           var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bg_secondary);
1556      	--primary_text:           var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_fg);
1557      	--primary_text_h:         var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_title);
1558      	--headings_text:          var(--primary_text_h);
1559      	--headings_text_solid:    var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_title_solid);
1560      	--primary_bg_btn:         var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_bg);
1561      	--primary_bg_btn_dark:    var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_bg_dark);
1562      	--primary_btn_text:       var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_primary_btn_fg);
1563      	--secondary_bg_btn:       var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_secondary_btn_bg);
1564      	--secondary_bg_btn_dark:  var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_secondary_btn_bg_dark);
1565      	--secondary_btn_text:     var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_secondary_btn_fg);
1566      	--tertiary_bg_btn:        var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_tertiary_btn_bg);
1567      	--tertiary_bg_btn_dark:   var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_tertiary_btn_bg_dark);
1568      	--tertiary_btn_text:      var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_tertiary_btn_fg);
1569      	--custom_input_bg:        var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_input_bg);
1570      	--custom_input_fg:        var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_input_fg);
1571      	--custom_input_pl:        var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_input_pl);
1572      	--custom_input_bd:        var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_bd);
1573      	--custom_bd:              var(--custom_input_bd);
1574      	--secondary_bg: 		  var(--primary_bg_btn);
1575      	--accent:                 var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_accent);
1576      	--accent_gradient:        var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_accent_gradient);
1577      	--primary_text_brightness:var(--scheme-c800998b-c7f1-4f24-82e6-971865496e61_fg_brightness);
1578      	}
1579      	
1580      
1581    </style>
1582
1583    
1584      <!-- Google Tag Manager -->
1585      
1585<script>
vendor: 475 bytes, lines 1585-1595
1585
1586        (function (w, d, s, l, i) {
1587          w[l] = w[l] || [];
1588          w[l].push({ 'gtm.start': new Date().getTime(), event: 'gtm.js' });
1589          var f = d.getElementsByTagName(s)[0],
1590            j = d.createElement(s),
1591            dl = l != 'dataLayer' ? '&l=' + l : '';
1592          j.async = true;
1593          j.src = 'https://www.googletagmanager.com/gtm.js?id=' + i + dl;
1594          f.parentNode.insertBefore(j, f);
1595        })(window, document, 'script', 'dataLayer', '
1595GTM-TXKBT5M4
vendor: 10 bytes, lines 1595-1596
1595');
1596      
1596</script>
1596
1597      <!-- End Google Tag Manager -->
1598    
1599
1600    
1600<script>
1601      window.dataLayer = window.dataLayer || [];
1602      window.dataLayer.push({
1603        shopCurrency: 'USD',
1604        pageType: 'product',
1605        customerId: '',
1606      });
1607    </script>
1607
1608
1609    
1610
1611
1612<script>
1613  document.addEventListener("DOMContentLoaded", function(event) {
1614    const style = document.getElementById('wsg-custom-style');
1615    if (typeof window.isWsgCustomer != "undefined" && isWsgCustomer) {
1616      style.innerHTML = `
1617        ${style.innerHTML} 
1618        /* A friend of hideWsg - this will _show_ only for wsg customers. Add class to an element to use */
1619        .showWsg {
1620          display: unset;
1621        }
1622        /* wholesale only CSS */
1623        .additional-checkout-buttons, .shopify-payment-button {
1624          display: none !important;
1625        }
1626        .wsg-proxy-container select {
1627          background-color: 
1628          ${
1629        document.querySelector('input').style.backgroundColor
1630          ? document.querySelector('input').style.backgroundColor
1631          : 'white'
1632        } !important;
1633        }
1634      `;
1635    } else {
1636      style.innerHTML = `
1637        ${style.innerHTML}
1638        /* Add CSS rules here for NOT wsg customers - great to hide elements from retail when we can't access the code driving the element */
1639        
1640      `;
1641    }
1642
1643    if (typeof window.embedButtonBg !== undefined && typeof window.embedButtonText !== undefined && window.embedButtonBg !== window.embedButtonText) {
1644      style.innerHTML = `
1645        ${style.innerHTML}
1646        .wsg-button-fix {
1647          background: ${embedButtonBg} !important;
1648          border-color: ${embedButtonBg} !important;
1649          color: ${embedButtonText} !important;
1650        }
1651      `;
1652    }
1653
1654    // =========================
1655    //         CUSTOM JS
1656    // ==========================
1657    if (document.querySelector(".wsg-proxy-container")) {
1658      initNodeObserver(wsgCustomJs);
1659    }
1660  })
1661
1662  function wsgCustomJs() {
1663
1664    // update button classes
1665    const button = document.querySelectorAll(".wsg-button-fix");
1666    let buttonClass = "xxButtonClassesHerexx";
1667    buttonClass = buttonClass.split(" ");
1668    for (let i = 0; i < button.length; i++) {
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1670    }
1671
1672    // wsgCustomJs window placeholder
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1777    .wsg-center img {
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1790  .wsg-table {
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1793  .wsg-spof-container-main {
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1796  /* General fixes */
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2007
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vendor: 1 bytes, line 2008
2008
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2043        return document.cookie.indexOf('loggedConversion=' + token) !== -1;
2044      }
2045      return false;
2046    }
2047
2048    var setCookieIfConversion = function(token) {
2049      if (token) {
2050        var twoMonthsFromNow = new Date(Date.now());
2051        twoMonthsFromNow.setMonth(twoMonthsFromNow.getMonth() + 2);
2052
2053        document.cookie = 'loggedConversion=' + token + '; expires=' + twoMonthsFromNow;
2054      }
2055    }
2056
2057    var trekkie = window.ShopifyAnalytics.lib = window.trekkie = window.trekkie || [];
2058    window.ShopifyAnalytics.lib.trekkie = window.trekkie;
2059    if (trekkie.integrations) {
2060      return;
2061    }
2062    trekkie.methods = [
2063      'identify',
2064      'page',
2065      'ready',
2066      'track',
2067      'trackForm',
2068      'trackLink'
2069    ];
2070    trekkie.factory = function(method) {
2071      return function() {
2072        var args = Array.prototype.slice.call(arguments);
2073        args.unshift(method);
2074        trekkie.push(args);
2075        if (method == 'track' || method == 'page') {
2076          var pageUrl;
2077          try {
2078            pageUrl = window.location.href;
2079          } catch (e) {}
2080          var pageReferrer;
2081          try {
2082            pageReferrer = document.referrer;
2083          } catch (e) {}
2084          try {
2085            if (window.__TREKKIE_SHIM_QUEUE == null) {
2086              window.__TREKKIE_SHIM_QUEUE = [];
2087            }
2088            window.__TREKKIE_SHIM_QUEUE.push({
2089              from: 'trekkie-stub',
2090              method: method,
2091              args: args.slice(1),
2092              pageContext: {
2093                url: pageUrl,
2094                referrer: pageReferrer
2095              }
2096            });
2097          } catch (e) {
2098            // no-op
2099          }
2100        }
2101        return trekkie;
2102      };
2103    };
2104    for (var i = 0; i < trekkie.methods.length; i++) {
2105      var key = trekkie.methods[i];
2106      trekkie[key] = trekkie.factory(key);
2107    }
2108    trekkie.load = function(config) {
2109      trekkie.config = config || {};
2110      trekkie.config.initialDocumentCookie = document.cookie;
2111      var first = document.getElementsByTagName('script')[0];
2112var script = document.createElement('script');
2113script.type = 'text/javascript';
2114script.onerror = function(e) {
2115  var scriptFallback = document.createElement('script');
2116  scriptFallback.type = 'text/javascript';
2117  scriptFallback.onerror = function(error) {
2118          var Monorail = {
2119      produce: function produce(monorailDomain, schemaId, payload) {
2120        var currentMs = new Date().getTime();
2121        var event = {
2122          schema_id: schemaId,
2123          payload: payload,
2124          metadata: {
2125            event_created_at_ms: currentMs,
2126            event_sent_at_ms: currentMs
2127          }
2128        };
2129        return Monorail.sendRequest("https://" + monorailDomain + "/v1/produce", JSON.stringify(event));
2130      },
2131      sendRequest: function sendRequest(endpointUrl, payload) {
2132        // Try the sendBeacon API
2133        if (window && window.navigator && typeof window.navigator.sendBeacon === 'function' && typeof window.Blob === 'function' && !Monorail.isIos12()) {
2134          var blobData = new window.Blob([payload], {
2135            type: 'text/plain'
2136          });
2137
2138          if (window.navigator.sendBeacon(endpointUrl, blobData)) {
2139            return true;
2140          } // sendBeacon was not successful
2141
2142        } // XHR beacon
2143
2144        var xhr = new XMLHttpRequest();
2145
2146        try {
2147          xhr.open('POST', endpointUrl);
2148          xhr.setRequestHeader('Content-Type', 'text/plain');
2149          xhr.send(payload);
2150        } catch (e) {
2151          console.log(e);
2152        }
2153
2154        return false;
2155      },
2156      isIos12: function isIos12() {
2157        return window.navigator.userAgent.lastIndexOf('iPhone; CPU iPhone OS 12_') !== -1 || window.navigator.userAgent.lastIndexOf('iPad; CPU OS 12_') !== -1;
2158      }
2159    };
2160    Monorail.produce('monorail-edge.shopifysvc.com',
2161      'trekkie_storefront_load_errors/1.1',
2162      {shop_id: 71852359737,
2163      theme_id: 148618739769,
2164      app_name: "storefront",
2165      context_url: window.location.href,
2166      source_url: "//www.goldbio.com/cdn/s/trekkie.storefront.2f6d7d7ede7d24234881a9cd5f47e679642fa1f6.min.js"});
2167
2168  };
2169  scriptFallback.async = true;
2170  scriptFallback.src = '//www.goldbio.com/cdn/s/trekkie.storefront.2f6d7d7ede7d24234881a9cd5f47e679642fa1f6.min.js';
2171  first.parentNode.insertBefore(scriptFallback, first);
2172};
2173script.async = true;
2174script.src = '//www.goldbio.com/cdn/s/trekkie.storefront.2f6d7d7ede7d24234881a9cd5f47e679642fa1f6.min.js';
2175first.parentNode.insertBefore(script, first);
2176
2177    };
2178    trekkie.load(
2179      {"Trekkie":{"appName":"storefront","development":false,"defaultAttributes":{"shopId":71852359737,"isMerchantRequest":null,"themeId":148618739769,"themeCityHash":"11557834470593795019","contentLanguage":"en","currency":"USD","eventMetadataId":"04518c3d-9eb1-4a5b-8d30-e62d9619ad78"},"isServerSideCookieWritingEnabled":true,"monorailRegion":"shop_domain","enabledBetaFlags":["764d78cb","8450a54b","7ee89bf1","4c64608a"]},"Session Attribution":{},"S2S":{"facebookCapiEnabled":false,"source":"trekkie-storefront-renderer","apiClientId":580111}}
2180    );
2181
2182    var loaded = false;
2183    trekkie.ready(function() {
2184      if (loaded) return;
2185      loaded = true;
2186
2187      window.ShopifyAnalytics.lib = window.trekkie;
2188
2189      var originalDocumentWrite = document.write;
2190      document.write = customDocumentWrite;
2191      try { window.ShopifyAnalytics.merchantGoogleAnalytics.call(this); } catch(error) {};
2192      document.write = originalDocumentWrite;
2193
2194      var match = window.location.pathname.match(/checkouts\/(.+)\/(thank_you|post_purchase)/)
2195      var token = match? match[1]: undefined;
2196      if (!hasLoggedConversion(token)) {
2197        setCookieIfConversion(token);
2198        window.ShopifyAnalytics.lib.track("Viewed Product",{"currency":"USD","variantId":44854634610745,"productId":8011355488313,"productGid":"gid:\/\/shopify\/Product\/8011355488313","name":"IPTG - 1 g","price":"27.00","sku":"I2481C","brand":"GoldBio","variant":"1 g","category":"","nonInteraction":true,"remote":false,"available":true},undefined,undefined,{"shopifyEmitted":true});
2199      }
2200    });
2201
2202    window.ShopifyAnalytics.lib.page(null,{"pageType":"product","resourceType":"product","resourceId":8011355488313,"requestId":"b6e94186-5a6b-4f2e-9501-4420642b5b54-1790917233","shopifyEmitted":true});
2203
2204    var eventsListenerScript = document.createElement('script');
2205    eventsListenerScript.async = true;
2206    eventsListenerScript.src = "//www.goldbio.com/cdn/shopifycloud/storefront/assets/shop_events_listener-4e26a9ce.js";
2207    document.getElementsByTagName('head')[0].appendChild(eventsListenerScript);
2208})();</script>
vendor: 1 bytes, line 2208
2208
2209<script
2210  defer
2211  src="https://www.goldbio.com/cdn/shopifycloud/perf-kit/shopify-perf-kit-3.9.4.min.js"
2212  data-application="storefront-renderer"
2213  data-shop-id="71852359737"
2214  data-render-region="gcp-us-central1"
2215  data-page-type="product"
2216  data-theme-instance-id="148618739769"
2217  data-theme-name="Xtra"
2218  data-theme-version="6.0.0"
2219  data-monorail-region="shop_domain"
2220  data-resource-timing-sampling-rate="10"
2221  data-shs="true"
2222  data-shs-beacon="true"
2223  data-shs-export-with-fetch="true"
2224  data-shs-logs-sample-rate="1"
2225  data-shs-beacon-endpoint="https://www.goldbio.com/api/collect"
2226></script>
2226
2227<meta name="shopify-y" content="fce03e2f-4e40-4696-8593-68e42e8f71ed" data-expiration="1822474833000"><meta name="shopify-s" content="38168d2f-d029-4af2-9877-38d048e83898" data-expiration="1790919033000"><meta name="new-cookie-storage-activated" content="t">
2228</head>
2229  <body class="template-product">
2230    
2231      <!-- Google Tag Manager (noscript) -->
vendor: 93 bytes, lines 2231-2234
2231
2232      <noscript
2233        ><iframe
2234          src="https://www.googletagmanager.com/ns.html?id=
2234GTM-TXKBT5M4
vendor: 135 bytes, lines 2234-2240
2234"
2235          height="0"
2236          width="0"
2237          style="display:none;visibility:hidden"
2238        ></iframe
2239      ></noscript>
2240      
2240<!-- End Google Tag Manager (noscript) -->
2241    
2242    <div id="fb-root"></div>
2243    
2243<script>
2244      (function (d, s, id) {
2245        var js,
2246          fjs = d.getElementsByTagName(s)[0];
2247        if (d.getElementById(id)) return;
2248        js = d.createElement(s);
2249        js.id = id;
2250        js.src = 'https://connect.facebook.net/en_US/sdk.js#xfbml=1&version=v2.5';
2251        fjs.parentNode.insertBefore(js, fjs);
2252      })(document, 'script', 'facebook-jssdk');
2253    </script>
2253
2254
2255    <div id="root"><nav id="skip">
2256    <ul>
2257        <li><a href="/" accesskey="a" class="link-accessible">Toggle accessibility mode (a)</a></li>
2258        <li><a href="/" accesskey="h">Go to homepage (h)</a></li>
2259        <li><a href="#nav" accesskey="n">Go to navigation (n)</a></li>
2260        <li><a href="/search" aria-controls="search" accesskey="s">Go to search (s)</a></li>
2261        <li><a href="#content" accesskey="c">Go to content (c)</a></li>
2262        <li><a href="#shopify-section-footer" accesskey="f">Go to footer (f)</a></li>
2263    </ul>
2264</nav><!-- BEGIN sections: header-group -->
2265<div id="shopify-section-sections--19420674097209__header" class="shopify-section shopify-section-group-header-group shopify-section-header">
2266<link href="//www.goldbio.com/cdn/shop/t/4/assets/header.css?v=118300044769976535391761666979" rel="stylesheet" type="text/css" media="all" />
2267
2268<script>document.documentElement.classList.add('search-compact-is-centered');</script>
2268
2269
2270<style>
2271  :root {
2272  --content_p:                  0px;
2273  
2274  --ghw:  1420px;
2275  
2276  --custom_top_up_bg:           var(--scheme-1_bg);
2277  --custom_top_up_fg:           var(--scheme-1_fg);
2278  --custom_top_up_fg_hover:     var(--scheme-1_btn_bg);
2279  --custom_top_up_h: 	 	35px;
2280  
2281  --header_mih:                 109px;
2282  --custom_top_main_bg:         var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bg);
2283  --custom_top_main_fg:         var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_fg);
2284      --custom_top_main_link_bg:    var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg);
2285      --custom_top_main_link_dark:  var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg_dark);
2286      --custom_top_main_link_text:  var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_fg);
2287    
2288  
2289  
2290   --custom_top_nav_h: 55px;
2291  --custom_top_nav_bg:          var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_bg);
2292  --custom_top_nav_scrollbar_bg:var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_fg_sat);
2293  --custom_top_nav_fz:          18px;
2294  --mobile_nav_fz:              14px;
2295  --custom_top_nav_ls:          0.0em;
2296  --custom_top_nav_fw:          600;
2297  
2298  
2299    --custom_top_main_sh: 0 9px 32px rgba(0,0,0,0.07);
2300  
2301  
2302--custom_top_nav_bd_op:       1.0;
2303    --custom_top_nav_bd:          rgb(236, 237, 236);
2304  
2305  --custom_top_nav_fg:          var(--scheme-1cc006fb-83b1-4713-9349-bd1a4cf7569d_fg);
2306  --custom_top_nav_fg_hover:    var(--custom_top_nav_fg);
2307  
2308--custom_top_search_bd:     var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_bd);--custom_top_search_bg:       var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_bg);
2309  --custom_top_search_fg:       var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_fg);
2310  --custom_top_search_pl:       var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_input_pl);
2311  }
2312  @media only screen and (max-width: 760px) {
2313  :root {
2314  --content_p:                  0px;
2315  --custom_top_search_h:        40px;
2316  }.shopify-section-header { --logo_w: 130px; }html #root .shopify-section-header #search.full:not(.no-autocomplete) > p, html #root .shopify-section-header #search.full:not(.no-autocomplete) > fieldset > p {
2317  border-color: var(--scheme-1_bg_secondary);
2318  background: var(--scheme-1_bg_secondary);
2319  }
2320  html #search > a.toggle, #search p > a.search-back {
2321  --custom_top_search_fg: var(--scheme-1_fg);
2322  }
2323  }
2324  @media only screen and (min-width: 761px) {
2325  :root {
2326  --custom_top_search_h:        clamp(calc(var(--main_fz) + 20px), 50px, calc(var(--header_mih) - 5px));
2327  --search_w:               390px;
2328  }
2329  #nav-bar > ul > li > ul > li > ul > li > a, #nav > ul > li > ul > li > ul > li > a { --main_fw: 400; font-weight: 400; }.shopify-section-header { --logo_w: 300px; }}
2330  @media only screen and (max-width: 62.5em ) {
2331  #shopify-section-sections--19420674097209__header #nav {
2332  --custom_drop_nav_bg:         var(--scheme-1_bg);
2333  --custom_drop_nav_fg:         var(--scheme-1_fg);
2334  --custom_drop_nav_fg_text:    var(--scheme-1_fg);
2335  --custom_drop_nav_fg_hover:   var(--scheme-1_btn_bg);
2336  --custom_drop_nav_head_bg:    var(--scheme-1_bg_var);
2337  --custom_drop_nav_head_fg:    var(--scheme-1_fg);
2338  --custom_drop_nav_bd:         var(--scheme-1_bd);
2339  }
2340  }
2341  @media only screen and (min-width: 62.5em ) {
2342  
2343  }
2344  #root #header .f8vl *:not(a[class*="overlay-"], button[class*="overlay-"]), #root .m6pn.f8vl *:not(a[class*="overlay-"], button[class*="overlay-"]) {
2345  --secondary_btn_text:             var(--scheme-1_btn_fg);
2346  --secondary_bg_btn:               var(--scheme-1_btn_bg);
2347  --secondary_bg_btn_dark:          var(--scheme-1_btn_bg_dark);
2348  --secondary_bg_btn_fade:          var(--scheme-1_btn_bg_dark);
2349  --white:                      var(--scheme-1_btn_fg);
2350  }
2351  :root {
2352  --header_mih:                 90px;
2353  --custom_top_up_h:       25px;
2354  }
2355  #root .shopify-section-header #header-inner > .link-btn a:first-child:after {
2356  content: "\e99a";
2357  }
2358  }
2359  #root #header .f8vl .submit, #root .m6pn.f8vl .submit {
2360  --secondary_btn_text:             var(--scheme-1_btn_fg);
2361  --secondary_bg_btn:               var(--scheme-1_btn_bg);
2362  --secondary_bg_btn_dark:          var(--scheme-1_btn_bg_dark);
2363  --secondary_bg_btn_fade:          var(--scheme-1_btn_bg_dark);
2364  --secondary_bg_fade:          var(--secondary_bg_btn_fade);
2365  }
2366  
2367    #nav.plain:before, #nav-bar.plain:before { border-top-width: 0!important; }
2368  
2369  
2370  #root .icon-cart:before { content: "\e903"; }
2371  #nav-user li:not(.link-btn) {
2372  
2373  
2374    --custom_top_main_link_bg:    var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg);
2375    --custom_top_main_link_dark:  var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_bg_dark);
2376    --custom_top_main_link_text:  var(--scheme-f84d67c2-3148-40bf-b6db-e141fd84f067_primary_btn_fg);
2377  
2378  }
2379  
2380</style>
2381
2382<div id="header-outer">
2383  <div id="header">
2384    <div
2385      id="header-inner"
2386      class="
2387        
2388        
2389        
2390        mobile-visible-search
2391        text-center-mobile
2392        
2393        
2394        logo-text
2395      "
2396    ><p id="logo" class="">
2397    <a href="/"><picture><img src="//www.goldbio.com/cdn/shop/files/New_Logo_R.png?v=1784317653&amp;width=600" alt="GoldBio" srcset="//www.goldbio.com/cdn/shop/files/New_Logo_R.png?v=1784317653&amp;width=300 300w, //www.goldbio.com/cdn/shop/files/New_Logo_R.png?v=1784317653&amp;width=600 600w" width="300" height="134.12526997840172" class="logo-img" sizes="(min-width: 760px) 300px, 130px">
2398        </picture></a><style>
2399        #logo {
2400        --logo_w: 300px;
2401        }
2402        @media only screen and (max-width: 47.5em) {
2403        #logo {
2404        --logo_w: 130px;
2405        }
2406        }
2407      </style></p>
2408<p class="link-btn">
2409    <a class="text-justify mobile-text-uppercase" href="/" aria-controls="nav">View catalog <i aria-hidden="true" class="icon-chevron-down"></i></a>
2410    <a class="search-compact" href="#search" aria-label="search" aria-controls="search">
2411      
2412        <i aria-hidden="true" class="icon-zoom"></i> <span class="hidden">Search</span>
2413      
2414    </a>
2415  </p>
2416<form action="/search" method="get" id="search" class="
2417    
2418    text-center-sticky
2419    
2420    
2421    no-autocomplete
2422    blur solid
2423    no-bg bd-b no-pd-t
2424    compact-handle
2425    "
2426        style="--main_fz:14px;--price_fz:14px;font-size:14px;--placeholder_fz:14px;"
2427  >
2428    <fieldset>
2429      <legend>Search</legend>
2430      <p>
2431        <label for="search_main">Search</label>
2432        <input type="search" id="search_main" name="q" placeholder="What are you looking for?" autocomplete="off" required>
2433        <button type="submit" class="override">Zoeken</button>
2434      </p>
2435      <div id="livesearch"><div class="cols">
2436            <p>Search results</p>
2437          </div></div>
2438    </fieldset>
2439  </form>
2440<nav
2441        id="nav-user"
2442        
2443      >
2444        <ul data-type="user-nav">
2445              
2446                <li class="desktop-only mobile-hide">
2447                  <div class="cite"><a href="/pages/citation-search-tool"><img src="https://cdn.shopify.com/s/files/1/0718/5235/9737/files/1-Graduation_Cap_-_Citation_Count-01.png?v=1760980849" width="80px" style="float:left"><div class="prodCite">1000+ PRODUCTS CITED</div></a></div>
2448<style>
2449.prodCite {
2450margin-top: 10px;
2451color: white;
2452}
2453.cite:hover {
2454text-decoration: underline;
2455}
2456</style>
2457                </li>
2458                
2459              
2460            
2461
2462            <li class="mobile-only">
2463              <a
2464                href=""
2465                
2466                  role="none" style="pointer-events: none"
2467                
2468              >
2469                
2470                
2471              </a>
2472            </li>
2473          
2474
2475<li class="sub  user-login menu-hide "><a
2476                  href="https://www.goldbio.com/customer_authentication/redirect?locale=en&region_country=US"
2477                  class="mobile-hide"
2478                  aria-label="Sign in"
2479                >
2480                  
2481                    <i
2482                      aria-hidden="true"
2483                      aria-label="Sign in"
2484                      class="icon-user"
2485                    ></i>
2486            <!--        <span class="desktop-hide">Sign in</span>  -->
2487                                        <span>Sign in</span>
2488                  
2489                </a>
2490                <a
2491                  href="https://www.goldbio.com/customer_authentication/redirect?locale=en&region_country=US"
2492                  class="mobile-only"
2493                  aria-label="Sign in"
2494                  ><i aria-hidden="true" class="icon-user"></i>
2495                  <span class="hidden">Sign in</span></a
2496                >
2497                 
2498</li><li class="wishlist-header hidden">
2499            <a href="#wishlist" aria-label="Wishlist"><i class="icon-heart-outline"></i></a>
2500          </li>
2501          
2502            <li class="cart base-font">
2503              <a
2504                href="/cart"
2505                
2506                  data-panel="cart"
2507                
2508                aria-label="Cart $0.00"
2509              >
2510                
2511                  <span class="hidden">Cart</span
2512                  ><i
2513                    aria-hidden="true"
2514                    class="icon-cart"
2515                    ><span id="cart-count" class="plain">0</span></i
2516                  >
2517                
2518              </a>
2519            </li>
2520          
2521        </ul>
2522      </nav>
2523    </div>
2524  </div><nav
2525        id="nav-bar"
2526        aria-label="Menu"
2527        class="hr nav-scroll-wrapper text-left plain tr_bd ff-primary dropdown bm-a"
2528      >
2529        <ul
2530          data-type="horizontal-nav"
2531          
2532            class="nav-scroll no-scroll"
2533          
2534        >
2535<li class="  active text-uppercase " aria-current="page">
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3196        <h1 class="m1 size-28">IPTG</h1>          
3197
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3199
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3351              <li class="active">
3352                <a href="#section-info">Description</a>
3353              </li>
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3356
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3358            <div id="section-info">
3359              <h2 class="mobile-hide">Description</h2><p>IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in <em>E. coli</em>. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.</p>
3360<p>IPTG is commonly used in cloning procedures that require induction of β-galactosidase and is most often used with <a href="/products/x-gal" target="_blank">X-Gal (Gold Bio #X4281C)</a> or <a href="/products/930/bluo-gal" target="_blank">Bluo-Gal (Gold Bio #B-673)</a> for blue/white colony screening or <a href="/products/magenta-gal" target="_blank">Magenta-Gal (Gold Bio #B-378)</a> for red/white colony screening of bacterial colonies.</p>
3361<p>IPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).</p>
3362<p><strong>GoldBio IPTG is made from a synthetic, non-animal origin.</strong></p>
3363<p> </p>
3364<h2>Functional Highlights and Mechanism</h2>
3365<ul>
3366<li>
3367<p>IPTG acts by mimicking the natural inducer allolactose: it binds to the lac repressor (LacI) and causes its release from the lac operator site, allowing transcription of downstream genes under the lac promoter.<span class=""><span class="ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]"></span></span></p>
3368</li>
3369<li>
3370<p>Because IPTG is not metabolized by the cell, its concentration remains stable over time, enabling sustained induction rather than being depleted. <span class=""><span class="ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]"></span></span></p>
3371</li>
3372<li>
3373<p>In recombinant protein expression systems (for example vectors using T7 promoter under lac operon regulation), addition of IPTG triggers expression of the gene of interest downstream of the inducible promoter. <span class=""><span class="ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]"></span></span></p>
3374</li>
3375<li>
3376<p>IPTG is often used in colony screening (e.g., blue/white screening) in combination with chromogenic substrates such as X‑Gal: in this context IPTG activates β-galactosidase expression which cleaves X-Gal to yield a colored product. <span class=""><span class="ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]"></span></span></p>
3377</li>
3378<li>
3379<p>Because of the consistency and high quality of GoldBio’s IPTG, it supports reproducible gene induction workflows and reduces variability associated with induction reagent quality.</p>
3380</li>
3381</ul>
3382<p>
3382 </p>
3383<h2>Applications</h2>
3384<ul>
3385<li>Gene expression induction in systems controlled by the lac operon</li>
3386<li>Blue-white screening (with X-Gal) for recombinant DNA identification</li>
3387<li>Controlled production of recombinant proteins</li>
3388<li>Timed gene activation for functional studies</li>
3389<li>Regulatory input in synthetic biology circuits</li>
3390</ul>
3391<h2>Research Applications for IPTG</h2>
3392<p><em><strong>(Click each for more information)</strong></em></p>
3393<details>
3394<summary><strong>Induction of Protein Expression in Lac Operon-Based Systems <br></strong></summary>
3395<ul>
3396<li>
3397<strong>Purpose</strong>: To induce recombinant protein expression in bacterial systems, particularly <em>Escherichia coli</em>.</li>
3398<li>
3399<strong>How It Works</strong>: IPTG is a non-metabolizable analog of allolactose that binds to the LacI repressor, causing dissociation from the lac operator and activation of transcription from lac-derived promoters.</li>
3400<li>
3401<strong>Applications</strong>: High-yield recombinant protein production using pET, pGEX, pMAL, and related expression systems.</li>
3402</ul>
3403<p>Studier, F. W., &amp; Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. <em>Journal of Molecular Biology</em>, 189(1), 113–130.</p>
3404</details>
3405<details>
3406<summary><strong>Blue/White Screening in Cloning <br></strong></summary>
3407<ul>
3408<li>
3409<strong>Purpose</strong>: To differentiate recombinant clones from non-recombinant colonies during cloning workflows.</li>
3410<li>
3411<strong>How It Works</strong>: IPTG induces β-galactosidase expression; in the presence of X-gal, colonies with intact <em>lacZα</em> appear blue, while recombinant colonies with disrupted <em>lacZ</em> remain white.</li>
3412<li>
3413<strong>Applications</strong>: Screening workflows using pUC19, pBluescript, and other multiple cloning site-containing vectors.</li>
3414</ul>
3415<p>Vieira, J., &amp; Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. <em>Gene</em>, 19(3), 259–268.</p>
3416</details>
3417<details>
3418<summary><strong>Study of Gene Regulation and Promoter Function <br></strong></summary>
3419<ul>
3420<li>
3421<strong>Purpose</strong>: To investigate transcriptional regulation and promoter-controlled gene expression.</li>
3422<li>
3423<strong>How It Works</strong>: IPTG induces reporter genes such as GFP or luciferase downstream of lac-based promoters, enabling quantitative analysis of promoter activity and regulatory dynamics.</li>
3424<li>
3425<strong>Applications</strong>: Promoter mapping, transcriptional dynamics studies, functional genomics, and educational laboratory assays.</li>
3426</ul>
3427<p>Zaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., ... &amp; Alon, U. (2006). A comprehensive library of fluorescent transcriptional reporters for <em>Escherichia coli</em>. <em>Nature Methods</em>, 3(8), 623–628.</p>
3428</details>
3429<details>
3430<summary><strong>Metabolic Engineering and Pathway Control <br></strong></summary>
3431<ul>
3432<li>
3433<strong>Purpose</strong>: To enable temporal regulation of enzyme expression in engineered metabolic pathways.</li>
3434<li>
3435<strong>How It Works</strong>: Genes encoding pathway enzymes are placed under lac-derived promoters, allowing controlled pathway activation and metabolic flux modulation through IPTG induction.</li>
3436<li>
3437<strong>Applications</strong>: Production optimization for biofuels, amino acids, polymers, and specialty chemicals in engineered microbial systems.</li>
3438</ul>
3439<p>Zhang, F., Carothers, J. M., &amp; Keasling, J. D. (2012). Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. <em>Nature Biotechnology</em>, 30(4), 354–359.</p>
3440</details>
3441<details>
3442<summary><strong>Conditional Gene Expression in Synthetic Biology <br></strong></summary>
3443<ul>
3444<li>
3445<strong>Purpose</strong>: To provide external control over synthetic gene circuits and regulatory networks.</li>
3446<li>
3447<strong>How It Works</strong>: LacI-IPTG regulatory modules function as tunable switches, repressors, or oscillators within engineered biological systems.</li>
3448<li>
3449<strong>Applications</strong>: Synthetic circuit design, biosensor development, microbial consortia regulation, and educational synthetic biology platforms.</li>
3450</ul>
3451<p>Elowitz, M. B., &amp; Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. <em>Nature</em>, 403(6767), 335–338.</p>
3452</details>
3453<p> </p>
3454<h2>Reconstitution</h2>
3455<h4>100mM IPTG stock solution</h4>
3456<ol>
3457<li>Weigh 0.238 g of IPTG</li>
3458<li>Add 10 mL sterile H<sub>2</sub>O. Dissolve completely.</li>
3459<li>Prewet a 0.22 µm syringe filter by drawing through 5-10 mL of sterile H<sub>2</sub>O and discard water.</li>
3460<li>Sterilize IPTG Stock through the prepared 0.22 µm syringe filter.</li>
3461<li>Store in 1 mL aliquots at -20°C for up to 1 year.</li>
3462</ol>
3463<p>
3463 </p>
3464<h2>Storage/Handling</h2>
3465<p>Store powder IPTG desiccated at -20°C. Protect from light. <br>For IPTG stock solution, store in 1 mL aliquots at -20°C for up to 1 year. IPTG is readily dissolved in sterile water.</p>
3466<p> </p>
3467<h2>FAQ</h2>
3468<h3>How does IPTG work?</h3>
3469<p>IPTG, which is similar to the lactose metabolite allolactose, induces the lac operon by binding to the lac repressor. Once IPTG binds to the lac repressor, a conformational change occurs, causing the lac repressor to dissociate from the lac promoter. Once the lac repressor dissociates, RNA polymerase binds to the promoter to initiate expression.</p>
3470<h3>How much IPTG should be used for expression/ What concentration of IPTG should be used for expression?</h3>
3471<p>Most papers recommend using between 0.1-1.0mM IPTG for expression. However, the amount you should use depends on your proteins. However, it is best to run an IPTG titration with ranges between 0.01-1.0mM and compare using SDS-PAGE to determine the optimal concentration. Something to keep in mind is higher concentrations of IPTG can cause inclusion bodies.</p>
3472<h3>Why should I use IPTG over lactose?</h3>
3473<p>IPTG is <strong>non-metabolizable</strong>, so its concentration remains stable and doesn’t get consumed by the bacteria.<br></p>
3474<h3>When should I add IPTG during culture growth?</h3>
3475<p>You should usually add IPTG at the <strong>mid-log phase (OD600 ≈ 0.4–0.6)</strong>
3475 to ensure cells are healthy and dividing rapidly.</p>
3476<h3>Do I need to autoclave IPTG?<br>
3477</h3>
3478<p>No—<strong>filter sterilize only</strong>. IPTG is heat-sensitive and should not be autoclaved.<br></p>
3479<h3>Does IPTG degrade over time?<br>
3480</h3>
3481<p>IPTG is stable when stored properly (aliquoted at <strong>-20°C</strong>, protected from light), but repeated freeze-thaw cycles should be avoided.<br></p>
3482<h3>Can IPTG be reused after thawing?<br>
3483</h3>
3484<p>The best practice is to use single-use aliquots of IPTG. <strong>Do not refreeze</strong> IPTG leftovers to avoid degradation or contamination.<br></p>
3485<h3>Can IPTG be toxic to cells?<br>
3486</h3>
3487<p>IPTG can be toxic at high concentrations or in sensitive strains. Lowering the IPTG concentration or using auto-induction media can help.<span class="redactor-invisible-space"><br></span></p>
3488<p><br></p>
3489<h2>IPTG Tutorial Videos</h2>
3490<p><iframe src="https://www.youtube.com/embed/xxvkGX4C05k?feature=player_detailpage" width="576" height="324"> </iframe> <iframe src="https://www.youtube.com/embed/l7aL5lVEq0M?feature=player_detailpage" width="576" height="324"> </iframe><br></p>
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3527                  <div class="custom-value">I2481C
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3531                <div class="custom-label">
3532                  CAS Number:
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3535                  367-93-1
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3541
3542</div>
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3589                          
3590                          
3591                        >
3592                          1 g ( $27.00)
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3597                          value="44854634643513"
3598                          data-product-url="/products/iptg"
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3600                          
3601                          
3602                          
3603                        >
3604                          5 g ( $38.00)
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3609                          value="44854634676281"
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3611                          
3612                          
3613                          
3614                          
3615                        >
3616                          25 g ( $153.00)
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3621                          value="44854634709049"
3622                          data-product-url="/products/iptg"
3623                          
3624                          
3625                          
3626                          
3627                        >
3628                          50 g ( $237.00)
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3633                          value="44854634741817"
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3637                          
3638                          
3639                        >
3640                          100 g ( $437.00)
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3646                          data-product-url="/products/iptg"
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3648                          
3649                          
3650                          
3651                        >
3652                          200 g ( $836.00)
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3657                          value="44854634807353"
3658                          data-product-url="/products/iptg"
3659                          
3660                          
3661                          
3662                          
3663                        >
3664                          300 g ( $1,113.00)
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3670                          data-product-url="/products/iptg"
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3674                          
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3676                          400 g ( $1,446.00)
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3685                          
3686                          
3687                        >
3688                          500 g ( $1,740.00)
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3690                  id="f8pr-pickup-2"
3691                  class="f8pr-pickup pickup mobile-only size-12 hidden"
3692                  ><i aria-hidden="true" class="icon-pin"></i> Free pickup in
3693                  <a href="#pickup-availability" data-panel="pickup-availability" data-id="44854634610745">our shop(s)
3694                  </a></span
3695                >
3696                
3697
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3721<div class="product-availability-wrap" style="font-weight: bold;">For research use only. Not for food, drug, household, or cosmetic use. </div>
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3724                    <div class="product-availability-wrap">
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3726                      <div class="availability-value">In stock  </div> 
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3729
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3887</div>
3888<!-- END app snippet --></div>
3889            </div></div>
3890  </div><footer class="m6tb static desktop-hide product-description">
3891        <nav class="hidden">
3892          <ul>
3893            <li class="active">
3894              <a href="#section-info">Description</a>
3895            </li>
3896          </ul>
3897        </nav>
3898        <div>
3899          <div
3900            id="section-info"
3901            
3902          >
3903            <h2 class="mobile-hide">Description</h2><p>IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in <em>E. coli</em>. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.</p>
3904<p>IPTG is commonly used in cloning procedures that require induction of β-galactosidase and is most often used with <a href="/products/x-gal" target="_blank">X-Gal (Gold Bio #X4281C)</a> or <a href="/products/930/bluo-gal" target="_blank">Bluo-Gal (Gold Bio #B-673)</a> for blue/white colony screening or <a href="/products/magenta-gal" target="_blank">Magenta-Gal (Gold Bio #B-378)</a> for red/white colony screening of bacterial colonies.</p>
3905<p>IPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).</p>
3906<p><strong>GoldBio IPTG is made from a synthetic, non-animal origin.</strong></p>
3907<p> </p>
3908<h2>Functional Highlights and Mechanism</h2>
3909<ul>
3910<li>
3911<p>IPTG acts by mimicking the natural inducer allolactose: it binds to the lac repressor (LacI) and causes its release from the lac operator site, allowing transcription of downstream genes under the lac promoter.<span class=""><span class="ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]"></span></span></p>
3912</li>
3913<li>
3914<p>Because IPTG is not metabolized by the cell, its concentration remains stable over time, enabling sustained induction rather than being depleted. <span class=""><span class="ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]"></span></span></p>
3915</li>
3916<li>
3917<p>In recombinant protein expression systems (for example vectors using T7 promoter under lac operon regulation), addition of IPTG triggers expression of the gene of interest downstream of the inducible promoter. <span class=""><span class="ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]"></span></span></p>
3918</li>
3919<li>
3920<p>IPTG is often used in colony screening (e.g., blue/white screening) in combination with chromogenic substrates such as X‑Gal: in this context IPTG activates β-galactosidase expression which cleaves X-Gal to yield a colored product. <span class=""><span class="ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]"></span></span></p>
3921</li>
3922<li>
3923<p>Because of the consistency and high quality of GoldBio’s IPTG, it supports reproducible gene induction workflows and reduces variability associated with induction reagent quality.</p>
3924</li>
3925</ul>
3926<p>
3926 </p>
3927<h2>Applications</h2>
3928<ul>
3929<li>Gene expression induction in systems controlled by the lac operon</li>
3930<li>Blue-white screening (with X-Gal) for recombinant DNA identification</li>
3931<li>Controlled production of recombinant proteins</li>
3932<li>Timed gene activation for functional studies</li>
3933<li>Regulatory input in synthetic biology circuits</li>
3934</ul>
3935<h2>Research Applications for IPTG</h2>
3936<p><em><strong>(Click each for more information)</strong></em></p>
3937<details>
3938<summary><strong>Induction of Protein Expression in Lac Operon-Based Systems <br></strong></summary>
3939<ul>
3940<li>
3941<strong>Purpose</strong>: To induce recombinant protein expression in bacterial systems, particularly <em>Escherichia coli</em>.</li>
3942<li>
3943<strong>How It Works</strong>: IPTG is a non-metabolizable analog of allolactose that binds to the LacI repressor, causing dissociation from the lac operator and activation of transcription from lac-derived promoters.</li>
3944<li>
3945<strong>Applications</strong>: High-yield recombinant protein production using pET, pGEX, pMAL, and related expression systems.</li>
3946</ul>
3947<p>Studier, F. W., &amp; Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. <em>Journal of Molecular Biology</em>, 189(1), 113–130.</p>
3948</details>
3949<details>
3950<summary><strong>Blue/White Screening in Cloning <br></strong></summary>
3951<ul>
3952<li>
3953<strong>Purpose</strong>: To differentiate recombinant clones from non-recombinant colonies during cloning workflows.</li>
3954<li>
3955<strong>How It Works</strong>: IPTG induces β-galactosidase expression; in the presence of X-gal, colonies with intact <em>lacZα</em> appear blue, while recombinant colonies with disrupted <em>lacZ</em> remain white.</li>
3956<li>
3957<strong>Applications</strong>: Screening workflows using pUC19, pBluescript, and other multiple cloning site-containing vectors.</li>
3958</ul>
3959<p>Vieira, J., &amp; Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. <em>Gene</em>, 19(3), 259–268.</p>
3960</details>
3961<details>
3962<summary><strong>Study of Gene Regulation and Promoter Function <br></strong></summary>
3963<ul>
3964<li>
3965<strong>Purpose</strong>: To investigate transcriptional regulation and promoter-controlled gene expression.</li>
3966<li>
3967<strong>How It Works</strong>: IPTG induces reporter genes such as GFP or luciferase downstream of lac-based promoters, enabling quantitative analysis of promoter activity and regulatory dynamics.</li>
3968<li>
3969<strong>Applications</strong>: Promoter mapping, transcriptional dynamics studies, functional genomics, and educational laboratory assays.</li>
3970</ul>
3971<p>Zaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., ... &amp; Alon, U. (2006). A comprehensive library of fluorescent transcriptional reporters for <em>Escherichia coli</em>. <em>Nature Methods</em>, 3(8), 623–628.</p>
3972</details>
3973<details>
3974<summary><strong>Metabolic Engineering and Pathway Control <br></strong></summary>
3975<ul>
3976<li>
3977<strong>Purpose</strong>: To enable temporal regulation of enzyme expression in engineered metabolic pathways.</li>
3978<li>
3979<strong>How It Works</strong>: Genes encoding pathway enzymes are placed under lac-derived promoters, allowing controlled pathway activation and metabolic flux modulation through IPTG induction.</li>
3980<li>
3981<strong>Applications</strong>: Production optimization for biofuels, amino acids, polymers, and specialty chemicals in engineered microbial systems.</li>
3982</ul>
3983<p>Zhang, F., Carothers, J. M., &amp; Keasling, J. D. (2012). Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. <em>Nature Biotechnology</em>, 30(4), 354–359.</p>
3984</details>
3985<details>
3986<summary><strong>Conditional Gene Expression in Synthetic Biology <br></strong></summary>
3987<ul>
3988<li>
3989<strong>Purpose</strong>: To provide external control over synthetic gene circuits and regulatory networks.</li>
3990<li>
3991<strong>How It Works</strong>: LacI-IPTG regulatory modules function as tunable switches, repressors, or oscillators within engineered biological systems.</li>
3992<li>
3993<strong>Applications</strong>: Synthetic circuit design, biosensor development, microbial consortia regulation, and educational synthetic biology platforms.</li>
3994</ul>
3995<p>Elowitz, M. B., &amp; Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. <em>Nature</em>, 403(6767), 335–338.</p>
3996</details>
3997<p> </p>
3998<h2>Reconstitution</h2>
3999<h4>100mM IPTG stock solution</h4>
4000<ol>
4001<li>Weigh 0.238 g of IPTG</li>
4002<li>Add 10 mL sterile H<sub>2</sub>O. Dissolve completely.</li>
4003<li>Prewet a 0.22 µm syringe filter by drawing through 5-10 mL of sterile H<sub>2</sub>O and discard water.</li>
4004<li>Sterilize IPTG Stock through the prepared 0.22 µm syringe filter.</li>
4005<li>Store in 1 mL aliquots at -20°C for up to 1 year.</li>
4006</ol>
4007<p>
4007 </p>
4008<h2>Storage/Handling</h2>
4009<p>Store powder IPTG desiccated at -20°C. Protect from light. <br>For IPTG stock solution, store in 1 mL aliquots at -20°C for up to 1 year. IPTG is readily dissolved in sterile water.</p>
4010<p> </p>
4011<h2>FAQ</h2>
4012<h3>How does IPTG work?</h3>
4013<p>IPTG, which is similar to the lactose metabolite allolactose, induces the lac operon by binding to the lac repressor. Once IPTG binds to the lac repressor, a conformational change occurs, causing the lac repressor to dissociate from the lac promoter. Once the lac repressor dissociates, RNA polymerase binds to the promoter to initiate expression.</p>
4014<h3>How much IPTG should be used for expression/ What concentration of IPTG should be used for expression?</h3>
4015<p>Most papers recommend using between 0.1-1.0mM IPTG for expression. However, the amount you should use depends on your proteins. However, it is best to run an IPTG titration with ranges between 0.01-1.0mM and compare using SDS-PAGE to determine the optimal concentration. Something to keep in mind is higher concentrations of IPTG can cause inclusion bodies.</p>
4016<h3>Why should I use IPTG over lactose?</h3>
4017<p>IPTG is <strong>non-metabolizable</strong>, so its concentration remains stable and doesn’t get consumed by the bacteria.<br></p>
4018<h3>When should I add IPTG during culture growth?</h3>
4019<p>You should usually add IPTG at the <strong>mid-log phase (OD600 ≈ 0.4–0.6)</strong>
4019 to ensure cells are healthy and dividing rapidly.</p>
4020<h3>Do I need to autoclave IPTG?<br>
4021</h3>
4022<p>No—<strong>filter sterilize only</strong>. IPTG is heat-sensitive and should not be autoclaved.<br></p>
4023<h3>Does IPTG degrade over time?<br>
4024</h3>
4025<p>IPTG is stable when stored properly (aliquoted at <strong>-20°C</strong>, protected from light), but repeated freeze-thaw cycles should be avoided.<br></p>
4026<h3>Can IPTG be reused after thawing?<br>
4027</h3>
4028<p>The best practice is to use single-use aliquots of IPTG. <strong>Do not refreeze</strong> IPTG leftovers to avoid degradation or contamination.<br></p>
4029<h3>Can IPTG be toxic to cells?<br>
4030</h3>
4031<p>IPTG can be toxic at high concentrations or in sensitive strains. Lowering the IPTG concentration or using auto-induction media can help.<span class="redactor-invisible-space"><br></span></p>
4032<p><br></p>
4033<h2>IPTG Tutorial Videos</h2>
4034<p><iframe src="https://www.youtube.com/embed/xxvkGX4C05k?feature=player_detailpage" width="576" height="324"> </iframe> <iframe src="https://www.youtube.com/embed/l7aL5lVEq0M?feature=player_detailpage" width="576" height="324"> </iframe><br></p>
4035</div>
4036        </div>
4037      </footer>
4038
4039</article>
4040
4041
4042
4043  
4044  
4045  
4046    <div class="specification-section" id="specification-section-tab">
4047      <header class="section-header-border cols">
4048        <div class="title-styling heading">
4049          <h2>Product Specifications</h2>
4050        </div>
4051      </header>
4052      
4053          <div  class="product_desc_infochart">
4054          <table>
4055            <tbody>
4056              
4057                
4058                <tr>
4059                  <th>Catalog ID:</th>
4060                  <td>I2481</td>
4061                </tr>
4062              
4063              
4064               
4065                 <tr>
4066                  <th>Name(s):</th>
4067                  <td>IPTG;  Isopropyl-beta-D-thiogalactoside <br></td>
4068                 </tr>
4069               
4070               
4071               
4072               
4073                <tr>
4074                  <th>CAS #:</th>
4075                  <td>367-93-1</td>
4076                </tr>
4077               
4078               
4079                
4080                 <tr>
4081                  <th>Formula:</th>
4082                  <td>C<sub>9</sub>H<sub>18</sub>O<sub>5</sub>S1</td>
4083                </tr>
4084               
4085               
4086                
4087                  <tr>
4088                   <th>MW:</th>
4089                   <td>238.30 g/mol</td>
4090                  </tr>
4091              
4092              
4093              
4094                 <tr>
4095                   <th>Grade:</th>
4096                   <td>MOLECULAR BIOLOGY GRADE</td>
4097                 </tr>
4098              
4099              
4100              
4101                <tr>
4102                   <th>Storage/handling:</th>
4103                   <td>Store desiccated at -20°C. Protect from light.</td>
4104                 </tr>
4105              
4106              
4107
4108               
4109
4110                
4111
4112                
4113
4114                
4115
4116                
4117
4118                
4119
4120                
4121
4122                
4123
4124                
4125
4126                
4127
4128                
4129
4130                
4131               
4132              
4133
4134                
4135
4136                
4137
4138                
4139
4140                
4141
4142                
4143
4144                  
4145
4146                         
4147
4148                         
4149
4150                         
4151
4152                         
4153
4154                
4155
4156                    
4157
4158                    
4159
4160                    
4161
4162                    
4163
4164               
4165
4166              
4167              
4168              
4169              
4170                <tr>
4171                   <th>PubChem Chemical ID:</th>
4172                   <td><a href="http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=656894" target="_blank">656894</a></td>
4173                 </tr>
4174              
4175            </tbody>
4176          </table>
4177            </div>
4178          
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4181
4182
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4242
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4244  <header class="section-header-border cols"><div class="title-styling heading">
4245        <h2>Technical Documentation</h2>
4246      </div></header>
4247
4248  <div class="technical-documentation-wrap">
4249    <div class="doc-label">
4250      <span class="doc-badge badge-1"> P </span>
4251      Protocol
4252    </div>
4253
4254    <div class="doc-label">
4255      <span class="doc-badge badge-2"> O </span>
4256      Other
4257    </div>
4258
4259    <div class="doc-label">
4260      <span class="doc-badge badge-3"> C </span>
4261      Certificate of Analysis
4262    </div>
4263
4264    <div class="doc-label">
4265      <span class="doc-badge badge-4"> S </span>
4266      Safety Data Sheet
4267    </div>
4268  </div>
4269  <div class="technical-documentation">
4270      
4271        <div class="technical-document">
4272          <a class="technical-document-btn" href="//www.goldbio.com/cdn/shop/files/SDS_MSDS_-I2481.pdf?v=12331435468770360604" target="_blank">
4273            <div class="technical-document-icon ">
4274              
4275                
4276                
4277                  <div class="doc-label">
4278                    <span class="doc-badge badge-4"> S </span>
4279                  </div>
4280                
4281              
4282            </div>
4283            <div class="technical-document-detail">
4284              <h3 class="technical-document-name size-16">SDS (MSDS) I2481</h3>
4285              <div class="technical-document-description">
4286                <p>Safety Data Sheet - I2481</p>
4287              </div>
4288            </div>
4289          </a>
4290        </div>
4291      
4292        <div class="technical-document">
4293          <a class="technical-document-btn" href="//www.goldbio.com/cdn/shop/files/I2481-Spec-Sheet.pdf?v=15854614065118170554" target="_blank">
4294            <div class="technical-document-icon ">
4295              
4296                
4297                
4298                  <div class="doc-label">
4299                    <span class="doc-badge badge-3"> C </span>
4300                  </div>
4301                
4302              
4303            </div>
4304            <div class="technical-document-detail">
4305              <h3 class="technical-document-name size-16">I2481 Specification Sheet</h3>
4306              <div class="technical-document-description">
4307                <p>Specification Sheet - I2481</p>
4308              </div>
4309            </div>
4310          </a>
4311        </div>
4312      
4313        <div class="technical-document">
4314          <a class="technical-document-btn" href="//www.goldbio.com/cdn/shop/files/Blue_20White_20Screening_20of_20Bacterial_20Colonies_20using_20X-Gal_20and_20IPTG_20Plates.pdf?v=3974791636807413789" target="_blank">
4315            <div class="technical-document-icon ">
4316              
4317                
4318                  <div class="doc-label">
4319                    <span class="doc-badge badge-1"> P </span>
4320                  </div>
4321                
4322                
4323              
4324            </div>
4325            <div class="technical-document-detail">
4326              <h3 class="technical-document-name size-16">Blue-White Screening of Bacterial Colonies Utilizing X-Gal and IPTG Plates</h3>
4327              <div class="technical-document-description">
4328                <p><p>Protocol for adding X-Gal and IPTG to agar or plates for Blue/White screening method of bacterial transformation</p></p>
4329              </div>
4330            </div>
4331          </a>
4332        </div>
4333      
4334        <div class="technical-document">
4335          <a class="technical-document-btn" href="//www.goldbio.com/cdn/shop/files/IPTG_20Stability_20Report.pdf?v=9360711382150576611" target="_blank">
4336            <div class="technical-document-icon ">
4337              
4338                
4339                
4340                  <div class="doc-label">
4341                    <span class="doc-badge badge-2"> O </span>
4342                  </div>
4343                
4344              
4345            </div>
4346            <div class="technical-document-detail">
4347              <h3 class="technical-document-name size-16">IPTG Stability Report</h3>
4348              <div class="technical-document-description">
4349                <p><p>IPTG - Stability Report 0-36 months</p></p>
4350              </div>
4351            </div>
4352          </a>
4353        </div>
4354      
4355        <div class="technical-document">
4356          <a class="technical-document-btn" href="//www.goldbio.com/cdn/shop/files/IPTG_20Stock_20Solution.pdf?v=13340529216866576847" target="_blank">
4357            <div class="technical-document-icon ">
4358              
4359                
4360                  <div class="doc-label">
4361                    <span class="doc-badge badge-1"> P </span>
4362                  </div>
4363                
4364                
4365              
4366            </div>
4367            <div class="technical-document-detail">
4368              <h3 class="technical-document-name size-16">IPTG Stock Solution</h3>
4369              <div class="technical-document-description">
4370                <p><p>IPTG Stock Solution - 100 X (100mM)</p></p>
4371              </div>
4372            </div>
4373          </a>
4374        </div>
4375      
4376        <div class="technical-document">
4377          <a class="technical-document-btn" href="//www.goldbio.com/cdn/shop/files/IPTG_20Induction_20and_20Extraction_20of_20Proteins_20Protocol.pdf?v=15614010842119071872" target="_blank">
4378            <div class="technical-document-icon ">
4379              
4380                
4381                  <div class="doc-label">
4382                    <span class="doc-badge badge-1"> P </span>
4383                  </div>
4384                
4385                
4386              
4387            </div>
4388            <div class="technical-document-detail">
4389              <h3 class="technical-document-name size-16">IPTG Induction and Extraction of Proteins Protocol</h3>
4390              <div class="technical-document-description">
4391                <p><p>IPTG Induction & Extraction of Proteins - by Arur and Nayak. Schedl Lab, Wash U. St. Louis</p></p>
4392              </div>
4393            </div>
4394          </a>
4395        </div>
4396      
4397  </div>
4398</div>
4399
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4512  
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4515        
4516          <img
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4531    </figure>
4532  
4533  <div class="blog-post-content">
4534    <h3 class="article-title size-20">
4535      
4536     Using Multiple Antibiotics in Bacterial Culture
4537    </h3><p class="article-description body-small-text ">
4538          Antibiotics are important tools used to select for particular cell populations. For example, when bacteria are transformed with a plasmid, antibiotics are used to kill...
4539        </p>
4540<p class="show-full-article link"><a href="/blogs/articles/using-multiple-antibiotics-in-bacterial-culture" class="overlay-primary no-before inline strong"><span>Read more</span></a></p>
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4550          <img
4551            src="//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?v=1770320319"
4552            srcset=",//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=165 165w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=240 240w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=350 350w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=533 533w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=720 720w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=940 940w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=1066 1066w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=1280 1280w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=1400 1400w,//www.goldbio.com/cdn/shop/articles/0_-_IPTG_Stock_Solution_Thumbnail_-_1-8-2026_e82f1893-72b7-4a5b-aeff-5a4a03b5d838.jpg?pad_color=fff&v=1770320319&width=1700 1700w"
4553            sizes="
4554              
4555              (min-width: 760px) calc(100vw * 0.5)
4556              100vw
4557            "
4558            width="410"
4559            height="310"
4560            alt="How to make IPTG Stock Solution - Banner Image"
4561            loading="lazy"
4562          >
4563        
4564      </picture>
4565    </figure>
4566  
4567  <div class="blog-post-content">
4568    <h3 class="article-title size-20">
4569      
4570     How to Make an IPTG Stock Solution
4571    </h3><p class="article-description body-small-text ">
4572          IPTG is frequently used for blue/white colony screening and to induce protein expression in bacterial cultures. Fortunately, IPTG is very stable and easy to work...
4573        </p>
4574<p class="show-full-article link"><a href="/blogs/articles/how-to-make-an-iptg-stock-solution" class="overlay-primary no-before inline strong"><span>Read more</span></a></p>
4575    
4576  </div>
4577</li>
4578
4579<li class="blog-post ">
4580  
4581    <figure>
4582      <picture>
4583        
4584          <img
4585            src="//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?v=1770320590"
4586            srcset=",//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=165 165w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=240 240w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=350 350w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=533 533w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=720 720w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=940 940w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=1066 1066w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=1280 1280w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=1400 1400w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=1700 1700w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=2000 2000w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=2300 2300w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=2600 2600w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_induction_vs._auto-induction_thumbnail.png?pad_color=fff&v=1770320590&width=2900 2900w"
4587            sizes="
4588              
4589              (min-width: 760px) calc(100vw * 0.5)
4590              100vw
4591            "
4592            width="410"
4593            height="310"
4594            alt="IPTG vs. Auto-Induction thumbnail image"
4595            loading="lazy"
4596          >
4597        
4598      </picture>
4599    </figure>
4600  
4601  <div class="blog-post-content">
4602    <h3 class="article-title size-20">
4603      
4604     IPTG Induction vs Auto-Induction
4605    </h3><p class="article-description body-small-text ">
4606          I had been optimizing my protein’s expression for months and I thought that I had already tried nearly everything that could be done. Still, I...
4607        </p>
4608<p class="show-full-article link"><a href="/blogs/articles/iptg-induction-vs-auto-induction" class="overlay-primary no-before inline strong"><span>Read more</span></a></p>
4609    
4610  </div>
4611</li>
4612
4613<li class="blog-post ">
4614  
4615    <figure>
4616      <picture>
4617        
4618          <img
4619            src="//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?v=1770320281"
4620            srcset=",//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=165 165w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=240 240w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=350 350w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=533 533w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=720 720w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=940 940w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=1066 1066w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=1280 1280w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=1400 1400w,//www.goldbio.com/cdn/shop/articles/0-_IPTG_Optimization_Thumbnail_d81e9ae2-c9e1-47a2-809a-da0209d900fc.jpg?pad_color=fff&v=1770320281&width=1700 1700w"
4621            sizes="
4622              
4623              (min-width: 760px) calc(100vw * 0.5)
4624              100vw
4625            "
4626            width="410"
4627            height="310"
4628            alt="IPTG optimization thumbnail - iptg tubes"
4629            loading="lazy"
4630          >
4631        
4632      </picture>
4633    </figure>
4634  
4635  <div class="blog-post-content">
4636    <h3 class="article-title size-20">
4637      
4638     Optimizing Protein Expression with IPTG Induction
4639    </h3><p class="article-description body-small-text ">
4640          Isopropyl b-D-1-thiogalactopyranoside (IPTG) induces recombinant protein expression in bacteria. But have you ever used your lab’s standard IPTG induction protocol and been disappointed with your...
4641        </p>
4642<p class="show-full-article link"><a href="/blogs/articles/optimizing-protein-expression-with-iptg-induction" class="overlay-primary no-before inline strong"><span>Read more</span></a></p>
4643    
4644  </div>
4645</li>
4646
4647<li class="blog-post ">
4648  
4649    <figure>
4650      <picture>
4651        
4652          <img
4653            src="//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?v=1778678707"
4654            srcset=",//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=165 165w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=240 240w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=350 350w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=533 533w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=720 720w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=940 940w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=1066 1066w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=1280 1280w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=1400 1400w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=1700 1700w,//www.goldbio.com/cdn/shop/articles/0-Banner-_20What_20DE3_20means_20in_20BL21_20DE3_20and_20DL39_20DE3_20Competent_20Cells_20_e9d9a070-3230-4200-9566-efd2cd2756d4.jpg?pad_color=fff&v=1778678707&width=2000 2000w"
4655            sizes="
4656              
4657              (min-width: 760px) calc(100vw * 0.5)
4658              100vw
4659            "
4660            width="410"
4661            height="310"
4662            alt="What Does the DE3 Represent in Competent Cells Like BL21(DE3) and DL39(DE3)?*"
4663            loading="lazy"
4664          >
4665        
4666      </picture>
4667    </figure>
4668  
4669  <div class="blog-post-content">
4670    <h3 class="article-title size-20">
4671      
4672     What Does the DE3 Represent in Competent Cells Like BL21(DE3) and DL39(DE3)?*
4673    </h3><p class="article-description body-small-text ">
4674          Expression systems are important tools in molecular biology, enabling researchers to produce proteins for research, diagnostics, and therapeutics. Examples of E. coli protein expression strains...
4675        </p>
4676<p class="show-full-article link"><a href="/blogs/articles/what-does-the-de3-represent-in-competent-cells-like-bl21de3-and-dl39de3" class="overlay-primary no-before inline strong"><span>Read more</span></a></p>
4677    
4678  </div>
4679</li>
4680
4681<li class="blog-post ">
4682  
4683    <figure>
4684      <picture>
4685        
4686          <img
4687            src="//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?v=1786649490"
4688            srcset=",//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=165 165w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=240 240w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=350 350w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=533 533w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=720 720w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=940 940w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=1066 1066w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=1280 1280w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=1400 1400w,//www.goldbio.com/cdn/shop/articles/0-Banner_-_Alpha-complementation_like_i_m_10_banner_3320bc50-6e23-4333-940b-6810f6c4b955.jpg?pad_color=fff&v=1786649490&width=1700 1700w"
4689            sizes="
4690              
4691              (min-width: 760px) calc(100vw * 0.5)
4692              100vw
4693            "
4694            width="410"
4695            height="310"
4696            alt="Explain Alpha-Complementation and Blue-White Screening To Me Like I’m 10"
4697            loading="lazy"
4698          >
4699        
4700      </picture>
4701    </figure>
4702  
4703  <div class="blog-post-content">
4704    <h3 class="article-title size-20">
4705      
4706     Explain Alpha-Complementation and Blue-White Screening To Me Like I’m 10
4707    </h3><p class="article-description body-small-text ">
4708          Alpha-complementation – it’s a big phrase, but it’s not terribly hard to understand. The technical explanation of alpha-complementation involves combining two parts of a protein,...
4709        </p>
4710<p class="show-full-article link"><a href="/blogs/articles/explain-alpha-complementation-and-blue-white-screening-like-im-10" class="overlay-primary no-before inline strong"><span>Read more</span></a></p>
4711    
4712  </div>
4713</li>
4714
4715<li class="blog-post ">
4716  
4717    <figure>
4718      <picture>
4719        
4720          <img
4721            src="//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?v=1786649465"
4722            srcset=",//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=165 165w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=240 240w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=350 350w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=533 533w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=720 720w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=940 940w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=1066 1066w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=1280 1280w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=1400 1400w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=1700 1700w,//www.goldbio.com/cdn/shop/articles/01-Banner_20Differential_20Media_b77d5f9b-b731-4485-9e18-578c7fce88c1.jpg?pad_color=fff&v=1786649465&width=2000 2000w"
4723            sizes="
4724              
4725              (min-width: 760px) calc(100vw * 0.5)
4726              100vw
4727            "
4728            width="410"
4729            height="310"
4730            alt="All About Differential Media in Gene Cloning - What it is and How it Works"
4731            loading="lazy"
4732          >
4733        
4734      </picture>
4735    </figure>
4736  
4737  <div class="blog-post-content">
4738    <h3 class="article-title size-20">
4739      
4740     All About Differential Media in Gene Cloning - What it is and How it Works
4741    </h3><p class="article-description body-small-text ">
4742          Differential media is culture media containing compounds that enable visual distinction between the microbes growing on it. These media leverage the distinct metabolic and biochemical...
4743        </p>
4744<p class="show-full-article link"><a href="/blogs/articles/all-about-differential-media-in-gene-cloning-what-it-is-and-how-it-works" class="overlay-primary no-before inline strong"><span>Read more</span></a></p>
4745    
4746  </div>
4747</li>
4748</ul></div>
4749
4750<style>
4751  @media only screen and (min-width: 47.5em) {
4752  
4753    #shopify-section-template--19564622053433__section_related_blog_posts_dNe48y { padding-block: 50px; margin-bottom: 0; }
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5379                  <a
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5436                    href="/pages/lab-startup"
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5439                    New Lab Startup Program
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5481                    Help Center
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5484                  <a
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5509                    New Lab Startup Program
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5512  <div class="bottom-bar-store-info">
5513    <div class="store-info">
5514      <p class="store-name"> GoldBio &copy; <span class="date">2026</span> All Rights Reserved  </p>
5515      <p class="store-name-link"> <a href="/policies/privacy-policy" title="Privacy Policy">Privacy Policy</a> </p>
5516    </div>
5517     <div class="store-other-info">
5518       Gold Biotechnology (U.S. Registration No 3,257,927) and Goldbio (U.S. Registration No 3,257,926) are registered trademarks of Gold Biotechnology, Inc.
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5802    }
5803
5804    let enabledCommonFeatures = '{"add-to-wishlist-collections-button":false,"add-to-wishlist-pdp-button":true,"sfl-popup-v2":true,"control-centre":true,"auto-add-to-wishlist-from-pdp":{"pdpViewCount":"3","notificationTimeoutDuration":"5","selectedPosition":"TopLeft","enabled":false},"multiple-wishlist":true,"social-count":{"enabledOnCollections":false}}';
5805    try {
5806      enabledCommonFeatures = JSON.parse(enabledCommonFeatures) || {};
5807    } catch (e) {
5808      enabledCommonFeatures = {};
5809    }
5810    // Storing COMMON FEATURES data in the window object for potential use in the storefront JS code.
5811    window.SwymEnabledCommonFeatures = enabledCommonFeatures;
5812
5813    // Ghost-Intent Configuration
5814    // Defaults to disabled (false). Only enabled when metafields explicitly set to "true".
5815    try {
5816      let ghostIntentConfig = {
5817        isExplicitConsentNeeded: false,
5818        isExplicitConsentNeededEvenForLocalStorage: false,
5819        askForPermissionOnFirstClick: true
5820      };
5821      
5822      
5823      
5824      window.SwymGhostIntentConfig = ghostIntentConfig;
5825    } catch(e) {
5826      window.SwymGhostIntentConfig = {
5827        isExplicitConsentNeeded: false,
5828        isExplicitConsentNeededEvenForLocalStorage: false,
5829        askForPermissionOnFirstClick: true
5830      };
5831    }
5832
5833    // Education Layer Configuration
5834    // Reads from common enabled-features metafield first. Falls back to old standalone metafield for backward compatibility.
5835    
5836    
5837    window.SwymEducationLayerEnabled = enabledCommonFeatures?.["education-layer"] ?? true;
5838
5839    // Login Nudge Configuration
5840    // Reads from common enabled-features metafield first. Falls back to old standalone metafield for backward compatibility.
5841    
5842    
5843    window.SwymLoginNudgeEnabled = enabledCommonFeatures?.["login-nudge"] ?? true;
5844
5845    // Initialize or ensure SwymViewProducts exists
5846    if (!window.SwymViewProducts) {
5847      window.SwymViewProducts = {};
5848    }
5849    
5850      try {
5851        const socialCountMap = { "https://www.goldbio.com/products/iptg": { "socialCount": 32, "empi": 8011355488313 } };
5852        // Integrate social count data into existing SwymViewProducts structure
5853        Object.keys(socialCountMap).forEach(function(key) {
5854          if (!window.SwymViewProducts[key]) {
5855            window.SwymViewProducts[key] = {};
5856          }
5857
5858          window.SwymViewProducts[key] = socialCountMap[key];
5859        });
5860      } catch (e) {
5861        // Silent error handling
5862      }
5863    
5864  })();
5865</script>
5865
5866
5867<!-- BEGIN app snippet: swymLoadConsentManager -->
5868<script>
5869  (function () {
5870    if (document.querySelector("script[data-swym-consent-manager]")) return;
5871    var swymConsentManagerScript = document.createElement("script");
5872    swymConsentManagerScript.src = "https:\/\/cdn.shopify.com\/extensions\/01a0f142-9ec3-73a2-babd-8904a7e44cee\/swym-relay-840\/assets\/swym-consent-manager.js";
5873    swymConsentManagerScript.async = false; // run in insertion order once fetched, instead of whenever fetch resolves
5874    swymConsentManagerScript.setAttribute("data-swym-consent-manager", "true");
5875    document.head.appendChild(swymConsentManagerScript);
5876  })();
5877</script>
5877
5878<!-- END app snippet -->
5879
5880<script
5881  
5882  id="wishlist-embed-init"
5883  defer
5884  async
5885>
5886  (function () {
5887    window.swymWishlistEmbedLoaded = true;
5888    var fullAssetUrl = "https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/apps.bundle.js"; 
5889    var assetBaseUrl = fullAssetUrl?.substring(0, fullAssetUrl.lastIndexOf('/') + 1);
5890    var swymJsPath = '//procdn.swymrelay.com/code/swym-shopify.js';
5891    var baseJsPath = swymJsPath?.substring(0, swymJsPath.lastIndexOf('/') + 1);
5892    window.SwymCurrentJSPath = baseJsPath;
5893    window.SwymAssetBaseUrl = assetBaseUrl;
5894    
5895      window.SwymCurrentStorePath = "//swymstore-v3pro-01.swymrelay.com";
5896    
5897    function loadSwymShopifyScript() {
5898      var element = "";
5899      var scriptSrc = "";
5900
5901      
5902        element = "swym-ext-shopify-script";
5903        window.SwymShopifyCdnInUse = true;
5904        scriptSrc = "https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/swym-ext-shopify.js";
5905      
5906
5907      if (document.getElementById(element)) {
5908        return;
5909      }
5910
5911      var s = document.createElement("script");
5912      s.id = element;
5913      s.type = "text/javascript";
5914      s.async = true;
5915      s.defer = true;
5916      s.src = scriptSrc;
5917
5918      s.onerror = function() {
5919        console.warn("Failed to load Swym Shopify script: ", scriptSrc, " Continuing with default");
5920        // Fallback logic here
5921        element = `swym-ext-shopify-script-${__SWYM__VERSION__}`;
5922        var fallbackJsPathVal = "\/\/procdn.swymrelay.com\/code\/swym-shopify.js";
5923        var fallbackJsPathWithExt = fallbackJsPathVal.replace("swym-shopify", "swym-ext-shopify");
5924        scriptSrc = fallbackJsPathWithExt + '?shop=' + encodeURIComponent(window.Shopify.shop) + '&v=' + __SWYM__VERSION__;
5925
5926        var fallbackScript = document.createElement("script");
5927        fallbackScript.id = element;
5928        fallbackScript.type = "text/javascript";
5929        fallbackScript.async = true;
5930        fallbackScript.defer = true;
5931        fallbackScript.src = scriptSrc;
5932        var y = document.getElementsByTagName("script")[0];
5933        y.parentNode.insertBefore(fallbackScript, y);
5934      };
5935
5936      var x = document.getElementsByTagName("script")[0];
5937      x.parentNode.insertBefore(s, x);
5938    }
5939    
5940      var consentAPICallbackInvoked = false;
5941      function checkConsentAndLoad() {
5942        // Allow app to load in design mode (theme editor) regardless of consent
5943        if (window.Shopify?.designMode) {
5944          loadSwymShopifyScript();
5945          return;
5946        }
5947
5948        // Ghost-Intent: If merchant enabled explicit consent, block SDK until
5949        // consent is given through our Ghost-Intent flow (sync nudge accept).
5950        // This works on any store regardless of region (EU or non-EU).
5951        var ghostConfig = window.SwymGhostIntentConfig || {};
5952        if (ghostConfig.isExplicitConsentNeeded || ghostConfig.isExplicitConsentNeededEvenForLocalStorage) {
5953          try {
5954            var consentKey = 'swym-ghost-consent-' + (window.Shopify && window.Shopify.shop || '');
5955            if (localStorage.getItem(consentKey) === 'true') {
5956              // Consent was given through our flow — load SDK
5957              loadSwymShopifyScript();
5958              return;
5959            }
5960          } catch(e) {}
5961
5962          // Check if Shopify native cookie consent was given via the cookie banner.
5963          // If user accepted cookies through Shopify's banner, load SDK normally
5964          // (Ghost-Intent is only needed when NO consent mechanism has been used).
5965          var shopifyConsentGiven = window.Shopify?.customerPrivacy?.preferencesProcessingAllowed?.();
5966          if (shopifyConsentGiven) {
5967            loadSwymShopifyScript();
5968            return;
5969          }
5970
5971          console.warn("[Swym Ghost-Intent] SDK blocked until explicit consent is given");
5972          return;
5973        }
5974
5975        var isCookieBannerVisible = window.Shopify?.customerPrivacy?.shouldShowBanner?.();
5976        if(!isCookieBannerVisible) {
5977          loadSwymShopifyScript();
5978          return;
5979        }
5980        var shouldLoadSwymScript = window.Shopify?.customerPrivacy?.preferencesProcessingAllowed?.();
5981        if (shouldLoadSwymScript) {
5982          loadSwymShopifyScript();
5983        } else {
5984          console.warn("No customer consent to load Swym Wishlist Plus");
5985        }
5986      }
5987      function initialiseConsentCheck() {
5988        document.addEventListener("visitorConsentCollected", (event) => { checkConsentAndLoad(); });
5989        window.Shopify?.loadFeatures?.(
5990          [{name: 'consent-tracking-api', version: '0.1'}],
5991          error => { 
5992            consentAPICallbackInvoked = true;
5993            if (error) {
5994              if(!window.Shopify?.customerPrivacy) {
5995                loadSwymShopifyScript();
5996                return;
5997              }
5998            }
5999            checkConsentAndLoad();
6000          }
6001        );
6002      }
6003      function consentCheckFallback(retryCount) {
6004        if(!consentAPICallbackInvoked) {
6005          if (window.Shopify?.customerPrivacy) {
6006            checkConsentAndLoad();
6007          } else if (retryCount >= 1) {
6008            console.warn("Shopify.loadFeatures unsuccessful on site, refer - https://shopify.dev/docs/api/customer-privacy#loading-the-customer-privacy-api. Proceeding with normal Swym Wishlist Plus load");
6009            // Ghost-Intent: route through checkConsentAndLoad so SDK block is respected
6010            checkConsentAndLoad();
6011          } else {
6012            setTimeout(() => consentCheckFallback(retryCount + 1), 1000);
6013          }
6014        }
6015      }
6016      if (document.readyState === "loading") {
6017        document.addEventListener("DOMContentLoaded", initialiseConsentCheck);
6018        window.addEventListener("load", () => consentCheckFallback(0));
6019      } else {
6020        initialiseConsentCheck();
6021      }
6022    
6023  })();
6024</script>
6024
6025
6026<!-- BEGIN app snippet: swymSnippet -->
6026<script defer>
6027  (function () {
6028    const currentSwymJSPath = '//procdn.swymrelay.com/code/swym-shopify.js';
6029    const currentSwymStorePath = 'https://swymstore-v3pro-01.swymrelay.com';
6030    const dnsPrefetchLink = `<link rel="dns-prefetch" href="${currentSwymStorePath}" crossorigin>`;
6031    const dnsPrefetchLink2 = `<link rel="dns-prefetch" href="${currentSwymJSPath}">`;
6032    const preConnectLink = `<link rel="preconnect" href="${currentSwymJSPath}">`;
6033    const swymSnippet = document.getElementById('wishlist-embed-init');        
6034    if(dnsPrefetchLink) {swymSnippet.insertAdjacentHTML('afterend', dnsPrefetchLink);}
6035    if(dnsPrefetchLink2) {swymSnippet.insertAdjacentHTML('afterend', dnsPrefetchLink2);}
6036    if(preConnectLink) {swymSnippet.insertAdjacentHTML('afterend', preConnectLink);}
6037  })()
6038</script>
vendor: 1 bytes, line 6038
6038
6039<script id="swym-snippet" type="text">
6040  window.swymLandingURL = document.URL;
6041  window.swymCart = {"note":null,"attributes":{},"original_total_price":0,"total_price":0,"total_discount":0,"total_weight":0.0,"item_count":0,"items":[],"requires_shipping":false,"currency":"USD","items_subtotal_price":0,"cart_level_discount_applications":[],"checkout_charge_amount":0};
6042  window.swymPageLoad = function() {
6043    window.SwymProductVariants = window.SwymProductVariants || {};
6044    window.SwymHasCartItems = 0 > 0;
6045    window.SwymPageData = {}, window.SwymProductInfo = {};var variants = [];
6046      window.SwymProductInfo.product = {"id":8011355488313,"title":"IPTG","handle":"iptg","description":"\u003cp\u003eIPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in \u003cem\u003eE. coli\u003c\/em\u003e. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.\u003c\/p\u003e\n\u003cp\u003eIPTG is commonly used in cloning procedures that require induction of β-galactosidase and is most often used with \u003ca href=\"\/products\/x-gal\" target=\"_blank\"\u003eX-Gal (Gold Bio #X4281C)\u003c\/a\u003e or \u003ca href=\"\/products\/930\/bluo-gal\" target=\"_blank\"\u003eBluo-Gal (Gold Bio #B-673)\u003c\/a\u003e for blue\/white colony screening or \u003ca href=\"\/products\/magenta-gal\" target=\"_blank\"\u003eMagenta-Gal (Gold Bio #B-378)\u003c\/a\u003e for red\/white colony screening of bacterial colonies.\u003c\/p\u003e\n\u003cp\u003eIPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGoldBio IPTG is made from a synthetic, non-animal origin.\u
6046003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFunctional Highlights and Mechanism\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eIPTG acts by mimicking the natural inducer allolactose: it binds to the lac repressor (LacI) and causes its release from the lac operator site, allowing transcription of downstream genes under the lac promoter.\u003cspan class=\"\"\u003e\u003cspan class=\"ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBecause IPTG is not metabolized by the cell, its concentration remains stable over time, enabling sustained induction rather than being depleted. \u003cspan class=\"\"\u003e\u003cspan class=\"ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIn recombinant protein expression systems (for example vectors using T7 promoter under lac operon regulation), addition of IPTG triggers expression of the gene of interest downstream of the inducible promoter. \u003cspan class=\"\"\u003e\u003cspan class=\"ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIPTG is often used in colony screening (e.g., blue\/white screening) in combination with chromogenic substrates such as X‑Gal: in this context IPTG activates β-galactosidase expression which cleaves X-Gal to yield a colored product. \u003cspan class=\"\"\u003e\u003cspan class=\"ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBecause of the consistency and high quality of GoldBio’s IPTG, it supports reproducible gene induction workflows and reduces variability associated with induction reagent quality.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression induction in systems controlled by the lac operon\u003c\/li\u003e\n\u003cli\u003eBlue-white screening (with X-Gal) for recombinant DNA identification\u003c\/li\u003e\n\u003cli\u003eControlled production of recombinant proteins\u003c\/li\u003e\n\u003cli\u003eTimed gene activation for functional studies\u003c\/li\u003e\n\u003cli\u003eRegulatory input in synthetic biology circuits\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eResearch Applications for IPTG\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e(Click each for more information)\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eInduction of Protein Expression in Lac Operon-Based Systems \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To induce recombinant protein expression in bacterial systems, particularly \u003cem\u003eEscherichia coli\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: IPTG is a non-metabolizable analog of allolactose that binds to the LacI repressor, causing dissociation from the lac operator and activation of transcription from lac-derived promoters.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: High-yield recombinant protein production using pET, pGEX, pMAL, and related expression systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStudier, F. W., \u0026amp; Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. \u003cem\u003eJournal of Molecular Biology\u003c\/em\u003e, 189(1), 113–130.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eBlue\/White Screening in Cloning \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To differentiate recombinant clones from non-recombinant colonies during cloning workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: IPTG induces β-galactosidase expression; in the presence of X-gal, colonies with intact \u003cem\u003elacZα\u003c\/em\u003e appear blue, while recombinant colonies with disrupted \u003cem\u003elacZ\u003c\/em\u003e remain white.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Screening workflows using pUC19, pBluescript, and other multiple cloning site-containing vectors.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eVieira, J., \u0026amp; Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. \u003cem\u003eGene\u003c\/em\u003e, 19(3), 259–268.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eStudy of Gene Regulation and Promoter Function \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To investigate transcriptional regulation and promoter-controlled gene expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: IPTG induces reporter genes such as GFP or luciferase downstream of lac-based promoters, enabling quantitative analysis of promoter activity and regulatory dynamics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Promoter mapping, transcriptional dynamics studies, functional genomics, and educational laboratory assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eZaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., ... \u0026amp; Alon, U. (2006). A comprehensive library of fluorescent transcriptional reporters for \u003cem\u003eEscherichia coli\u003c\/em\u003e. \u003cem\u003eNature Methods\u003c\/em\u003e, 3(8), 623–628.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eMetabolic Engineering and Pathway Control \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To enable temporal regulation of enzyme expression in engineered metabolic pathways.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: Genes encoding pathway enzymes are placed under lac-derived promoters, allowing controlled pathway activation and metabolic flux modulation through IPTG induction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Production optimization for biofuels, amino acids, polymers, and specialty chemicals in engineered microbial systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eZhang, F., Carothers, J. M., \u0026amp; Keasling, J. D. (2012). Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. \u003cem\u003eNature Biotechnology\u003c\/em\u003e, 30(4), 354–359.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eConditional Gene Expression in Synthetic Biology \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To provide external 
6046control over synthetic gene circuits and regulatory networks.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: LacI-IPTG regulatory modules function as tunable switches, repressors, or oscillators within engineered biological systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Synthetic circuit design, biosensor development, microbial consortia regulation, and educational synthetic biology platforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eElowitz, M. B., \u0026amp; Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. \u003cem\u003eNature\u003c\/em\u003e, 403(6767), 335–338.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003ch4\u003e100mM IPTG stock solution\u003c\/h4\u003e\n\u003col\u003e\n\u003cli\u003eWeigh 0.238 g of IPTG\u003c\/li\u003e\n\u003cli\u003eAdd 10 mL sterile H\u003csub\u003e2\u003c\/sub\u003eO. Dissolve completely.\u003c\/li\u003e\n\u003cli\u003ePrewet a 0.22 µm syringe filter by drawing through 5-10 mL of sterile H\u003csub\u003e2\u003c\/sub\u003eO and discard water.\u003c\/li\u003e\n\u003cli\u003eSterilize IPTG Stock through the prepared 0.22 µm syringe filter.\u003c\/li\u003e\n\u003cli\u003eStore in 1 mL aliquots at -20°C for up to 1 year.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\/Handling\u003c\/h2\u003e\n\u003cp\u003eStore powder IPTG desiccated at -20°C. Protect from light. \u003cbr\u003eFor IPTG stock solution, store in 1 mL aliquots at -20°C for up to 1 year. IPTG is readily dissolved in sterile water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003ch3\u003eHow does IPTG work?\u003c\/h3\u003e\n\u003cp\u003eIPTG, which is similar to the lactose metabolite allolactose, induces the lac operon by binding to the lac repressor. Once IPTG binds to the lac repressor, a conformational change occurs, causing the lac repressor to dissociate from the lac promoter. Once the lac repressor dissociates, RNA polymerase binds to the promoter to initiate expression.\u003c\/p\u003e\n\u003ch3\u003eHow much IPTG should be used for expression\/ What concentration of IPTG should be used for expression?\u003c\/h3\u003e\n\u003cp\u003eMost papers recommend using between 0.1-1.0mM IPTG for expression. However, the amount you should use depends on your proteins. However, it is best to run an IPTG titration with ranges between 0.01-1.0mM and compare using SDS-PAGE to determine the optimal concentration. Something to keep in mind is higher concentrations of IPTG can cause inclusion bodies.\u003c\/p\u003e\n\u003ch3\u003eWhy should I use IPTG over lactose?\u003c\/h3\u003e\n\u003cp\u003eIPTG is \u003cstrong\u003enon-metabolizable\u003c\/strong\u003e, so its concentration remains stable and doesn’t get consumed by the bacteria.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eWhen should I add IPTG during culture growth?\u003c\/h3\u003e\n\u003cp\u003eYou should usually add IPTG at the \u003cstrong\u003emid-log phase (OD600 ≈ 0.4–0.6)\u003c\/strong\u003e to ensure cells are healthy and divi
6046ding rapidly.\u003c\/p\u003e\n\u003ch3\u003eDo I need to autoclave IPTG?\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eNo—\u003cstrong\u003efilter sterilize only\u003c\/strong\u003e. IPTG is heat-sensitive and should not be autoclaved.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eDoes IPTG degrade over time?\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eIPTG is stable when stored properly (aliquoted at \u003cstrong\u003e-20°C\u003c\/strong\u003e, protected from light), but repeated freeze-thaw cycles should be avoided.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eCan IPTG be reused after thawing?\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eThe best practice is to use single-use aliquots of IPTG. \u003cstrong\u003eDo not refreeze\u003c\/strong\u003e IPTG leftovers to avoid degradation or contamination.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eCan IPTG be toxic to cells?\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eIPTG can be toxic at high concentrations or in sensitive strains. Lowering the IPTG concentration or using auto-induction media can help.\u003cspan class=\"redactor-invisible-space\"\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eIPTG Tutorial Videos\u003c\/h2\u003e\n\u003cp\u003e\u003ciframe src=\"https:\/\/www.youtube.com\/embed\/xxvkGX4C05k?feature=player_detailpage\" width=\"576\" height=\"324\"\u003e \u003c\/iframe\u003e \u003ciframe src=\"https:\/\/www.youtube.com\/embed\/l7aL5lVEq0M?feature=player_detailpage\" width=\"576\" height=\"324\"\u003e \u003c\/iframe\u003e\u003cbr\u003e\u003c\/p\u003e","published_at":"2025-09-09T14:20:08-05:00","created_at":"2025-09-09T14:20:07-05:00","vendor":"GoldBio","type":"","tags":["367931","I2481","UN"],"price":2700,"price_min":2700,"price_max":174000,"available":true,"price_varies":true,"compare_at_price":null,"compare_at_price_min":0,"compare_at_price_max":0,"compare_at_price_varies":false,"variants":[{"id":44854634610745,"title":"1 g","option1":"1 g","option2":null,"option3":null,"sku":"I2481C","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 1 g","public_title":"1 g","options":["1 g"],"price":2700,"weight":1,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44854634643513,"title":"5 g","option1":"5 g","option2":null,"option3":null,"sku":"I2481C5","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 5 g","public_title":"5 g","options":["5 g"],"price":3800,"weight":5,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44854634676281,"title":"25 g","option1":"25 g","option2":null,"option3":null,"sku":"I2481C25","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 25 g","public_title":"25 g","options":["25 g"],"price":15300,"weight":25,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44854634709049,"title":"50 g","option1":"50 g","option2":null,"option3":null,"sku":"I2481C50","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 50 g","public_title":"50 g","options":["50 g"],"price":23700,"weight":50,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44854634741817,"title":"100 g","option1":"100 g","option2":null,"option3":null,"sku":"I2481C100","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 100 g","public_title":"100 g","options":["100 g"],"price":43700,"weight":100,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44854634774585,"title":"200 g","option1":"200 g","option2":null,"option3":null,"sku":"I2481C200","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 200 g","public_title":"200 g","options":["200 g"],"price":83600,"weight":200,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44854634807353,"title":"300 g","option1":"300 g","option2":null,"option3":null,"sku":"I2481C300","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 300 g","public_title":"300 g","options":["300 g"],"price":111300,"weight":300,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44854634840121,"title":"400 g","option1":"400 g","option2":null,"option3":null,"sku":"I2481C400","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 400 g","public_title":"400 g","options":["400 g"],"price":144600,"weight":400,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}},{"id":44854634872889,"title":"500 g","option1":"500 g","option2":null,"option3":null,"sku":"I2481C500","requires_shipping":true,"taxable":true,"featured_image":null,"available":true,"name":"IPTG - 500 g","public_title":"500 g","options":["500 g"],"price":174000,"weight":500,"compare_at_price":null,"inventory_management":null,"barcode":null,"requires_selling_plan":false,"selling_plan_allocations":[],"quantity_rule":{"min":1,"max":null,"increment":1}}],"images":["\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620"],"featured_image":"\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620","options":["Size"],"media":[{"alt":null,"id":28569679102009,"position":1,"preview_image":{"aspect_ratio":1.0,"height":500,"width":500,"src":"\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620"},"aspect_ratio":1.0,"height":500,"media_type":"image","src":"\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?v=1757445620","width":500}],"requires_selling_plan":false,"selling_plan_groups":[],"content":"\u003cp\u003eIPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in \u003cem\u003eE. coli\u003c\/em\u003e. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.\u003c\/p\u003e\n\u003cp\u003eIPTG is commonly used in cloning procedures that require induction of β-galactosidase and is most often used with \u003ca href=\"\/products\/x-gal\" target=\"_blank\"\u003eX-Gal (Gold Bio #X4281C)\u003c\/a\u003e or \u003ca href=\"\/products\/930\/bluo-gal\" target=\"_blank\"\u003eBluo-Gal (Gold Bio #B-673)\u003c\/a\u003e for blue\/white colony screening or \u003ca href=\"\/products\/magenta-gal\" target=\"_blank\"\u003eMagenta-Gal (Gold Bio #B-378)\u003c\/a\u003e for red\/white colony screening of bacterial colonies.\u003c\/p\u003e\n\u003cp\u003eIPTG is also used in the induction of recombinant proteins. In those systems, a protein of interest is encoded downstream of the IPTG inducible promoter. In the presence of IPTG, the protein of interest is induced in the cell culture. The culture can then be lysed and the protein expressed and purified through a number of methods, including His Tag or GST purification systems (for proteins with ligand tags).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eGoldBio IPTG is made from a synthetic, non-animal origin.\u
6046003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFunctional Highlights and Mechanism\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eIPTG acts by mimicking the natural inducer allolactose: it binds to the lac repressor (LacI) and causes its release from the lac operator site, allowing transcription of downstream genes under the lac promoter.\u003cspan class=\"\"\u003e\u003cspan class=\"ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBecause IPTG is not metabolized by the cell, its concentration remains stable over time, enabling sustained induction rather than being depleted. \u003cspan class=\"\"\u003e\u003cspan class=\"ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIn recombinant protein expression systems (for example vectors using T7 promoter under lac operon regulation), addition of IPTG triggers expression of the gene of interest downstream of the inducible promoter. \u003cspan class=\"\"\u003e\u003cspan class=\"ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIPTG is often used in colony screening (e.g., blue\/white screening) in combination with chromogenic substrates such as X‑Gal: in this context IPTG activates β-galactosidase expression which cleaves X-Gal to yield a colored product. \u003cspan class=\"\"\u003e\u003cspan class=\"ms-1 inline-flex max-w-full items-center relative top-[-0.094rem] animate-[show_150ms_ease-in]\"\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBecause of the consistency and high quality of GoldBio’s IPTG, it supports reproducible gene induction workflows and reduces variability associated with induction reagent quality.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression induction in systems controlled by the lac operon\u003c\/li\u003e\n\u003cli\u003eBlue-white screening (with X-Gal) for recombinant DNA identification\u003c\/li\u003e\n\u003cli\u003eControlled production of recombinant proteins\u003c\/li\u003e\n\u003cli\u003eTimed gene activation for functional studies\u003c\/li\u003e\n\u003cli\u003eRegulatory input in synthetic biology circuits\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eResearch Applications for IPTG\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e(Click each for more information)\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eInduction of Protein Expression in Lac Operon-Based Systems \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To induce recombinant protein expression in bacterial systems, particularly \u003cem\u003eEscherichia coli\u003c\/em\u003e.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: IPTG is a non-metabolizable analog of allolactose that binds to the LacI repressor, causing dissociation from the lac operator and activation of transcription from lac-derived promoters.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: High-yield recombinant protein production using pET, pGEX, pMAL, and related expression systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStudier, F. W., \u0026amp; Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. \u003cem\u003eJournal of Molecular Biology\u003c\/em\u003e, 189(1), 113–130.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eBlue\/White Screening in Cloning \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To differentiate recombinant clones from non-recombinant colonies during cloning workflows.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: IPTG induces β-galactosidase expression; in the presence of X-gal, colonies with intact \u003cem\u003elacZα\u003c\/em\u003e appear blue, while recombinant colonies with disrupted \u003cem\u003elacZ\u003c\/em\u003e remain white.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Screening workflows using pUC19, pBluescript, and other multiple cloning site-containing vectors.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eVieira, J., \u0026amp; Messing, J. (1982). The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. \u003cem\u003eGene\u003c\/em\u003e, 19(3), 259–268.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eStudy of Gene Regulation and Promoter Function \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To investigate transcriptional regulation and promoter-controlled gene expression.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: IPTG induces reporter genes such as GFP or luciferase downstream of lac-based promoters, enabling quantitative analysis of promoter activity and regulatory dynamics.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Promoter mapping, transcriptional dynamics studies, functional genomics, and educational laboratory assays.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eZaslaver, A., Bren, A., Ronen, M., Itzkovitz, S., Kikoin, I., Shavit, S., ... \u0026amp; Alon, U. (2006). A comprehensive library of fluorescent transcriptional reporters for \u003cem\u003eEscherichia coli\u003c\/em\u003e. \u003cem\u003eNature Methods\u003c\/em\u003e, 3(8), 623–628.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eMetabolic Engineering and Pathway Control \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To enable temporal regulation of enzyme expression in engineered metabolic pathways.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: Genes encoding pathway enzymes are placed under lac-derived promoters, allowing controlled pathway activation and metabolic flux modulation through IPTG induction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Production optimization for biofuels, amino acids, polymers, and specialty chemicals in engineered microbial systems.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eZhang, F., Carothers, J. M., \u0026amp; Keasling, J. D. (2012). Design of a dynamic sensor-regulator system for production of chemicals and fuels derived from fatty acids. \u003cem\u003eNature Biotechnology\u003c\/em\u003e, 30(4), 354–359.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eConditional Gene Expression in Synthetic Biology \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To provide external 
6046control over synthetic gene circuits and regulatory networks.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: LacI-IPTG regulatory modules function as tunable switches, repressors, or oscillators within engineered biological systems.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Synthetic circuit design, biosensor development, microbial consortia regulation, and educational synthetic biology platforms.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eElowitz, M. B., \u0026amp; Leibler, S. (2000). A synthetic oscillatory network of transcriptional regulators. \u003cem\u003eNature\u003c\/em\u003e, 403(6767), 335–338.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eReconstitution\u003c\/h2\u003e\n\u003ch4\u003e100mM IPTG stock solution\u003c\/h4\u003e\n\u003col\u003e\n\u003cli\u003eWeigh 0.238 g of IPTG\u003c\/li\u003e\n\u003cli\u003eAdd 10 mL sterile H\u003csub\u003e2\u003c\/sub\u003eO. Dissolve completely.\u003c\/li\u003e\n\u003cli\u003ePrewet a 0.22 µm syringe filter by drawing through 5-10 mL of sterile H\u003csub\u003e2\u003c\/sub\u003eO and discard water.\u003c\/li\u003e\n\u003cli\u003eSterilize IPTG Stock through the prepared 0.22 µm syringe filter.\u003c\/li\u003e\n\u003cli\u003eStore in 1 mL aliquots at -20°C for up to 1 year.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\/Handling\u003c\/h2\u003e\n\u003cp\u003eStore powder IPTG desiccated at -20°C. Protect from light. \u003cbr\u003eFor IPTG stock solution, store in 1 mL aliquots at -20°C for up to 1 year. IPTG is readily dissolved in sterile water.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003ch3\u003eHow does IPTG work?\u003c\/h3\u003e\n\u003cp\u003eIPTG, which is similar to the lactose metabolite allolactose, induces the lac operon by binding to the lac repressor. Once IPTG binds to the lac repressor, a conformational change occurs, causing the lac repressor to dissociate from the lac promoter. Once the lac repressor dissociates, RNA polymerase binds to the promoter to initiate expression.\u003c\/p\u003e\n\u003ch3\u003eHow much IPTG should be used for expression\/ What concentration of IPTG should be used for expression?\u003c\/h3\u003e\n\u003cp\u003eMost papers recommend using between 0.1-1.0mM IPTG for expression. However, the amount you should use depends on your proteins. However, it is best to run an IPTG titration with ranges between 0.01-1.0mM and compare using SDS-PAGE to determine the optimal concentration. Something to keep in mind is higher concentrations of IPTG can cause inclusion bodies.\u003c\/p\u003e\n\u003ch3\u003eWhy should I use IPTG over lactose?\u003c\/h3\u003e\n\u003cp\u003eIPTG is \u003cstrong\u003enon-metabolizable\u003c\/strong\u003e, so its concentration remains stable and doesn’t get consumed by the bacteria.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eWhen should I add IPTG during culture growth?\u003c\/h3\u003e\n\u003cp\u003eYou should usually add IPTG at the \u003cstrong\u003emid-log phase (OD600 ≈ 0.4–0.6)\u003c\/strong\u003e to ensure cells are healthy and divi
6046ding rapidly.\u003c\/p\u003e\n\u003ch3\u003eDo I need to autoclave IPTG?\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eNo—\u003cstrong\u003efilter sterilize only\u003c\/strong\u003e. IPTG is heat-sensitive and should not be autoclaved.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eDoes IPTG degrade over time?\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eIPTG is stable when stored properly (aliquoted at \u003cstrong\u003e-20°C\u003c\/strong\u003e, protected from light), but repeated freeze-thaw cycles should be avoided.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eCan IPTG be reused after thawing?\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eThe best practice is to use single-use aliquots of IPTG. \u003cstrong\u003eDo not refreeze\u003c\/strong\u003e IPTG leftovers to avoid degradation or contamination.\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eCan IPTG be toxic to cells?\u003cbr\u003e\n\u003c\/h3\u003e\n\u003cp\u003eIPTG can be toxic at high concentrations or in sensitive strains. Lowering the IPTG concentration or using auto-induction media can help.\u003cspan class=\"redactor-invisible-space\"\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eIPTG Tutorial Videos\u003c\/h2\u003e\n\u003cp\u003e\u003ciframe src=\"https:\/\/www.youtube.com\/embed\/xxvkGX4C05k?feature=player_detailpage\" width=\"576\" height=\"324\"\u003e \u003c\/iframe\u003e \u003ciframe src=\"https:\/\/www.youtube.com\/embed\/l7aL5lVEq0M?feature=player_detailpage\" width=\"576\" height=\"324\"\u003e \u003c\/iframe\u003e\u003cbr\u003e\u003c\/p\u003e"};
6047      window.SwymProductInfo.variants = window.SwymProductInfo.product.variants;
6048      var piu = "\/\/www.goldbio.com\/cdn\/shop\/files\/I2481_ce074f3a-45ce-46d9-a471-938b6f531d43.jpg?crop=center\u0026height=620\u0026v=1
6048757445620\u0026width=620";
6049      
6050
6051        SwymProductVariants[44854634610745] = {
6052          empi: window.SwymProductInfo.product.id,epi:44854634610745,
6053          dt: "IPTG",
6054          du: "https://www.goldbio.com/products/iptg",
6055          iu:  piu ,
6056          stk:  1 ,
6057          pr: 2700/100,
6058          ct: window.SwymProductInfo.product.type,
6059          
6060          variants: [{ "1 g" : 44854634610745}]
6061        };
6062        SwymProductVariants[44854634643513] = {
6063          empi: window.SwymProductInfo.product.id,epi:44854634643513,
6064          dt: "IPTG",
6065          du: "https://www.goldbio.com/products/iptg",
6066          iu:  piu ,
6067          stk:  1 ,
6068          pr: 3800/100,
6069          ct: window.SwymProductInfo.product.type,
6070          
6071          variants: [{ "5 g" : 44854634643513}]
6072        };
6073        SwymProductVariants[44854634676281] = {
6074          empi: window.SwymProductInfo.product.id,epi:44854634676281,
6075          dt: "IPTG",
6076          du: "https://www.goldbio.com/products/iptg",
6077          iu:  piu ,
6078          stk:  1 ,
6079          pr: 15300/100,
6080          ct: window.SwymProductInfo.product.type,
6081          
6082          variants: [{ "25 g" : 44854634676281}]
6083        };
6084        SwymProductVariants[44854634709049] = {
6085          empi: window.SwymProductInfo.product.id,epi:44854634709049,
6086          dt: "IPTG",
6087          du: "https://www.goldbio.com/products/iptg",
6088          iu:  piu ,
6089          stk:  1 ,
6090          pr: 23700/100,
6091          ct: window.SwymProductInfo.product.type,
6092          
6093          variants: [{ "50 g" : 44854634709049}]
6094        };
6095        SwymProductVariants[44854634741817] = {
6096          empi: window.SwymProductInfo.product.id,epi:44854634741817,
6097          dt: "IPTG",
6098          du: "https://www.goldbio.com/products/iptg",
6099          iu:  piu ,
6100          stk:  1 ,
6101          pr: 43700/100,
6102          ct: window.SwymProductInfo.product.type,
6103          
6104          variants: [{ "100 g" : 44854634741817}]
6105        };
6106        SwymProductVariants[44854634774585] = {
6107          empi: window.SwymProductInfo.product.id,epi:44854634774585,
6108          dt: "IPTG",
6109          du: "https://www.goldbio.com/products/iptg",
6110          iu:  piu ,
6111          stk:  1 ,
6112          pr: 83600/100,
6113          ct: window.SwymProductInfo.product.type,
6114          
6115          variants: [{ "200 g" : 44854634774585}]
6116        };
6117        SwymProductVariants[44854634807353] = {
6118          empi: window.SwymProductInfo.product.id,epi:44854634807353,
6119          dt: "IPTG",
6120          du: "https://www.goldbio.com/products/iptg",
6121          iu:  piu ,
6122          stk:  1 ,
6123          pr: 111300/100,
6124          ct: window.SwymProductInfo.product.type,
6125          
6126          variants: [{ "300 g" : 44854634807353}]
6127        };
6128        SwymProductVariants[44854634840121] = {
6129          empi: window.SwymProductInfo.product.id,epi:44854634840121,
6130          dt: "IPTG",
6131          du: "https://www.goldbio.com/products/iptg",
6132          iu:  piu ,
6133          stk:  1 ,
6134          pr: 144600/100,
6135          ct: window.SwymProductInfo.product.type,
6136          
6137          variants: [{ "400 g" : 44854634840121}]
6138        };
6139        SwymProductVariants[44854634872889] = {
6140          empi: window.SwymProductInfo.product.id,epi:44854634872889,
6141          dt: "IPTG",
6142          du: "https://www.goldbio.com/products/iptg",
6143          iu:  piu ,
6144          stk:  1 ,
6145          pr: 174000/100,
6146          ct: window.SwymProductInfo.product.type,
6147          
6148          variants: [{ "500 g" : 44854634872889}]
6149        };window.SwymProductInfo.currentVariant = 44854634610745;
6150      var product_data = {
6151        et: 1, empi: window.SwymProductInfo.product.id, epi: window.SwymProductInfo.currentVariant,
6152        dt: "IPTG", du: "https://www.goldbio.com/products/iptg",
6153        ct: window.SwymProductInfo.product.type, pr: 2700/100,
6154        iu:  piu , 
6155        variants: [{ "1 g" : 44854634610745 }],
6156        stk:  1 
6157        
6158      };
6159      window.SwymPageData = product_data;
6160    
6161    window.SwymPageData.uri = window.swymLandingURL;
6162  };
6163  if(window.selectCallback){
6164    (function(){
6165      var originalSelectCallback = window.selectCallback;
6166      window.selectCallback = function(variant){
6167        originalSelectCallback.apply(this, arguments);
6168        try{
6169          if(window.triggerSwymVariantEvent){
6170            window.triggerSwymVariantEvent(variant.id);
6171          }
6172        }catch(err){
6173          console.warn("Swym selectCallback", err);
6174        }
6175      };})();}
6176  window.swymCustomerId =null;
6177  window.swymCustomerExtraCheck =
6178    null;
6179  var swappName = ("Wishlist" || "Wishlist");
6180  var swymJSObject = {
6181    pid: "axo01GCFLvAUJNLld54JuPHt\/ED6rqveoO7str+Z4r8=",
6182    interface: "/apps/swym" + swappName + "/interfaces/interfaceStore.php?appname=" + swappName
6183  };
6184  window.swymJSShopifyLoad = function(){
6185    if(window.swymPageLoad) swymPageLoad();
6186    if(!window._swat) {
6187      (function (s, w, r, e, l, a, y) {
6188        r['SwymRetailerConfig'] = s;
6189        r[s] = r[s] || function (k, v) {
6190          r[s][k] = v;
6191        };
6192      })('_swrc', '', window);
6193      _swrc('RetailerId', swymJSObject.pid);
6194      _swrc('Callback', function(){initSwymShopify();});
6195    }else if(window._swat.postLoader){
6196      _swrc = window._swat.postLoader;
6197      _swrc('RetailerId', swymJSObject.pid);
6198      _swrc('Callback', function(){initSwymShopify();});
6199    }else{
6200      initSwymShopify();}
6201  }
6202  if(!window._SwymPreventAutoLoad) {
6203    swymJSShopifyLoad();
6204  }
6205</script>
6205
6206
6207<style id="safari-flasher-pre"></style>
6208<script>
6209  if (navigator.userAgent.indexOf('Safari') != -1 && navigator.userAgent.indexOf('Chrome') == -1) {
6210    document.getElementById("safari-flasher-pre").innerHTML = '' + '#swym-plugin,#swym-hosted-plugin{display: none;}' + '.swym-button.swym-add-to-wishlist{display: none;}' + '.swym-button.swym-add-to-watchlist{display: none;}' + '#swym-plugin  #swym-notepad, #swym-hosted-plugin  #swym-notepad{opacity: 0; visibility: hidden;}' + '#swym-plugin  #swym-notepad, #swym-plugin  #swym-overlay, #swym-plugin  #swym-notification,' + '#swym-hosted-plugin  #swym-notepad, #swym-hosted-plugin  #swym-overlay, #swym-hosted-plugin  #swym-notification' + '{-webkit-transition: none; transition: none;}' + '';
6211    window.SwymCallbacks = window.SwymCallbacks || [];
6212    window.SwymCallbacks.push(function(tracker) {
6213      tracker.evtLayer.addEventListener(tracker.JSEvents.configLoaded, function() {
6214        var x = function() {
6215          SwymUtils.onDOMReady(function() {
6216            var d = document.createElement("div");
6217            d.innerHTML = "<style id='safari-flasher-post'>" + "#swym-plugin:not(.swym-ready),#swym-hosted-plugin:not(.swym-ready){display: none;}" + ".swym-button.swym-add-to-wishlist:not(.swym-loaded){display: none;}" + ".swym-button.swym-add-to-watchlist:not(.swym-loaded){display: none;}" + "#swym-plugin.swym-ready  #swym-notepad, #swym-plugin.swym-ready  #swym-overlay, #swym-plugin.swym-ready  #swym-notification," + "#swym-hosted-plugin.swym-ready  #swym-notepad, #swym-hosted-plugin.swym-ready  #swym-overlay, #swym-hosted-plugin.swym-ready  #swym-notification" + "{-webkit-transition: opacity 0.3s, visibility 0.3ms, -webkit-transform 0.3ms !important;-moz-transition: opacity 0.3s, visibility 0.3ms, -moz-transform 0.3ms !important;-ms-transition: opacity 0.3s, visibility 0.3ms, -ms-transform 0.3ms !important;-o-transition: opacity 0.3s, visibility 0.3ms, -o-transform 0.3ms !important;transition: opacity 0.3s, visibility 0.3ms, transform 0.3ms !important;}" + "</style>";
6218            document.head.appendChild(d);
6219          });};
6220        setTimeout(x, 10);
6221      });});}
6222  window.SwymOverrideMoneyFormat = "${{amount}}";
6223</script>
6223
6224<style id="swym-product-view-defaults"> .swym-button.swym-add-to-wishlist-view-product:not(.swym-loaded) { display: none; } </style><!-- END app snippet -->
6225
6226<script
6227  
6228  id="swymSnippetCheckAndActivate"
6229>
6230  (function () {
6231    var duplicateProneIds = ['safari-flasher-pre', 'swym-product-view-defaults', 'swym-wrap-login-handler'];
6232
6233    function removeDuplicateIdNodes(id) {
6234      var nodes = document.querySelectorAll('#' + id);
6235      nodes.forEach(function (node, index) {
6236        if (index > 0) {
6237          node.remove();
6238        }
6239      });
6240    }
6241
6242    function cleanupDuplicateSnippetNodes(activeScript) {
6243      if (!activeScript) {
6244        return;
6245      }
6246      var allSwymSnippets = document.querySelectorAll('script#swym-snippet');
6247      allSwymSnippets.forEach(function (scriptNode) {
6248        if (scriptNode !== activeScript) {
6249          scriptNode.remove();
6250        }
6251      });
6252    }
6253
6254    function cleanupDuplicateActivationGuards() {
6255      var guardScripts = document.querySelectorAll('script#swymSnippetCheckAndActivate');
6256      guardScripts.forEach(function (scriptNode, index) {
6257        if (index > 0) {
6258          scriptNode.removeAttribute('id');
6259        }
6260      });
6261    }
6262
6263    function postDomLoad() {
6264      var activeScript = document.querySelector('script#swym-snippet:not([type="text"])');
6265      if (!activeScript) {
6266        var inactiveScript = document.querySelector('script#swym-snippet[type="text"]');
6267        if (inactiveScript) {
6268          inactiveScript.type = 'text/javascript';
6269          new Function(inactiveScript.textContent)();
6270          activeScript = inactiveScript;
6271        }
6272      }
6273
6274      cleanupDuplicateSnippetNodes(activeScript);
6275      cleanupDuplicateActivationGuards();
6276      duplicateProneIds.forEach(removeDuplicateIdNodes);
6277    }
6278
6279    if (document.readyState === 'loading') {
6280      document.addEventListener('DOMContentLoaded', postDomLoad);
6281    } else {
6282      postDomLoad();
6283    }
6284  })();
6285</script>
6285
6286
6287
6288
6289<script>
6290  (function () {
6291    const themeSchemaName = window.Shopify.theme.schema_name;
6292
6293    if (!window.SwymCallbacks) {
6294      window.SwymCallbacks = [];
6295    }
6296    window.SwymCallbacks.push(swat => {
6297      if (!swat) {
6298        return;
6299      }
6300
6301      // Ghost-Intent: Sync local wishlist items to cloud when SDK loads after consent
6302      if (window.SwymLocalWishlist && window.SwymLocalWishlist.getSyncableItems().length > 0) {
6303        window.SwymLocalWishlist.syncToCloud(swat);
6304      }
6305
6306      if (window._SwymSDKOnlyMode) {
6307        return;
6308      }
6309
6310      // ENABLE COMMON FEATURES
6311      let swymEnabledCommonFeatures = window.SwymEnabledCommonFeatures;
6312      Object.keys(swymEnabledCommonFeatures).forEach(key => {
6313        if (!swymEnabledCommonFeatures[key]) return;
6314
6315        switch (key) {
6316          case 'add-to-wishlist-collections-button':
6317            // Check if app block is already handling the collections button
6318            if (window.SwymCollectionsConfig) {
6319              return;
6320            }
6321
6322            // Check if v2 default collections is handling it
6323            if (window.SwymAdvancedCollectionsWishlistConfig) {
6324              return;
6325            }
6326
6327            // Fallback to old v1 behavior if v2 config is not present
6328            swat?.collectionsApi?.setDefaultCustomizationOptions();
6329            swat?.collectionsApi?.initializeCollections(swat, false, themeSchemaName);
6330
6331            break;
6332          case 'add-to-wishlist-pdp-button':
6333            // Check if app block is already handling the PDP button
6334            if (window.swymATWButtonConfig?.shopifyExtensionType === 'appblock') {
6335              return;
6336            }
6337
6338            if (!(window.SwymPageData && window.SwymPageData.et === 1)) {
6339              // Not initiating pdp button as it is not a product page
6340              return;
6341            }
6342
6343            // Inject addtowishlist.css into the document's <head>
6344            var head = document.head;
6345            var pdpButtonStylesheet = "https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/addtowishlistbutton.css";
6346            var pdpButtonStylesheetTag = document.createElement('link');
6347            pdpButtonStylesheetTag.id = `swym-pdp-button-stylesheet`;
6348            pdpButtonStylesheetTag.rel = 'stylesheet';
6349            pdpButtonStylesheetTag.href = pdpButtonStylesheet;
6350
6351            if (!document.getElementById('swym-pdp-button-stylesheet')) {
6352              head.appendChild(pdpButtonStylesheetTag);
6353            }
6354
6355            var x = document.getElementsByTagName('script')[0];
6356            function createAndInsertScript(id, src, onLoadCallback) {
6357              var scriptTag = document.createElement('script');
6358              scriptTag.id = id;
6359              scriptTag.type = 'text/javascript';
6360              scriptTag.src = src;
6361              scriptTag.onload = onLoadCallback;
6362              x.parentNode.insertBefore(scriptTag, x);
6363            }
6364
6365            var pdpButtonScript = "https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/addtowishlistbutton.js";
6366            createAndInsertScript(`swym-pdp-button-script`, pdpButtonScript, function () {
6367              const pdpBtnApi = window.WishlistPlusPDPButtonAPI;
6368              if (pdpBtnApi) {
6369                pdpBtnApi.setDefaultCustomizationOptions(swat);
6370                pdpBtnApi.initializePDPButton(swat, true);
6371              }
6372            });
6373            break;
6374          default:
6375            return;
6376        }
6377      });
6378    });
6379  })(); // IIFE to prevent polluting global scope
6380</script>
6380
6381
6382
6383
6384<script> 
6385  (function () {
6386    // Get the settings from Shopify's Liquid variables and create the styles
6387    const isControlCentreEnabledFromMetafield = window?.SwymEnabledCommonFeatures?.["control-centre"];
6388    const isCommonCustomizationEnabledFromMetafield = window?.SwymWishlistCommonCustomizationSettings && 
6389      Object.keys(window.SwymWishlistCommonCustomizationSettings).length > 0;
6390    const isControlCentreEnabledFromBlockSettings = true;
6391    
6392    if (!(isControlCentreEnabledFromMetafield && isCommonCustomizationEnabledFromMetafield) && !isControlCentreEnabledFromBlockSettings) {
6393      // Don't enable control centre if this is disabled and block settings are not enabled
6394      return;
6395    }
6396    
6397    let borderRadius = 6;
6398    let drawerWidth = window?.SwymWishlistCommonCustomizationSettings?.storefrontLayoutDrawerWidth || 400;
6399    
6400    // Check if block settings exist, use them, otherwise fall back to common settings
6401    let primaryBgColor = isControlCentreEnabledFromBlockSettings ? 
6402      "#000000" : 
6403      window.SwymWishlistCommonCustomizationSettings.primaryColor;
6404    
6405    let primaryTextColor = isControlCentreEnabledFromBlockSettings ? 
6406      "#ffffff" : 
6407      window.SwymWishlistCommonCustomizationSettings.secondaryColor;
6408    
6409    let secondaryBgColor = isControlCentreEnabledFromBlockSettings ? 
6410      "#f4f8fe" : null;
6411    let secondaryTextColor = isControlCentreEnabledFromBlockSettings ? 
6412      "#333333" : null;
6413
6414    // Only use color adjustment functions if we're using common settings
6415    if (!secondaryBgColor || !secondaryTextColor) {
6416      function adjustOpacity(color, opacity) {
6417        if (color[0] === '#') {
6418          color = color.slice(1);
6419        }
6420
6421        if (color.length === 3) {
6422          color = color.split('').map(char => char + char).join('');
6423        }
6424
6425        const r = parseInt(color.slice(0, 2), 16);
6426        const g = parseInt(color.slice(2, 4), 16);
6427        const b = parseInt(color.slice(4, 6), 16);
6428
6429        return `rgba(${r}, ${g}, ${b}, ${opacity})`;
6430      }
6431
6432      function hexToRgb(color) {
6433        if (color[0] === '#') {
6434          color = color.slice(1);
6435        }
6436        if (color.length === 3) {
6437          color = color.split('').map(c => c + c).join('');
6438        }
6439
6440        return {
6441          r: parseInt(color.slice(0, 2), 16),
6442          g: parseInt(color.slice(2, 4), 16),
6443          b: parseInt(color.slice(4, 6), 16)
6444        };
6445      }
6446
6447      function getLuminance({ r, g, b }) {
6448        return 0.299 * r + 0.587 * g + 0.114 * b;
6449      }
6450
6451      function generateSecondaryColors(primaryBgColor, primaryTextColor) {
6452        const secondaryBgOpacity = 0.2;
6453        const secondaryTextOpacity = 0.8;
6454
6455        const secondaryBgColor = adjustOpacity(primaryBgColor, secondaryBgOpacity);
6456
6457        const bgRgb = hexToRgb(primaryBgColor);
6458        const bgLuminance = getLuminance(bgRgb);
6459
6460        // Determine whether to use dark or light text for contrast
6461        const lightText = adjustOpacity(primaryTextColor, secondaryTextOpacity);
6462        const darkText = adjustOpacity(primaryBgColor, secondaryTextOpacity);
6463
6464        const secondaryTextColor = bgLuminance > 186 ? darkText : lightText;
6465
6466        return {
6467          secondaryBgColor,
6468          secondaryTextColor
6469        };
6470      }
6471
6472      const generatedColors = generateSecondaryColors(primaryBgColor, primaryTextColor);
6473      secondaryBgColor = secondaryBgColor || generatedColors.secondaryBgColor;
6474      secondaryTextColor = secondaryTextColor || generatedColors.secondaryTextColor;
6475    }
6476
6477    // Create the CSS rule
6478    let styles = `
6479      .swym-storefront-layout-root-component {
6480        --swym-storefront-layout-ui-border-radius: ${borderRadius}px;
6481        --swym-storefront-layout-button-border-radius: ${borderRadius}px;
6482        --swym-storefront-layout-side-drawer-width: ${drawerWidth}px;
6483        --swym-storefront-layout-button-color-bg-primary: ${primaryBgColor};
6484        --swym-storefront-layout-button-color-text-primary: ${primaryTextColor};
6485        --swym-storefront-layout-button-color-bg-secondary: ${secondaryBgColor};
6486        --swym-storefront-layout-button-color-text-secondary: ${secondaryTextColor};
6487      }
6488    `;
6489
6490    // Create a <style> element and append the styles
6491    let styleSheet = document.createElement("style");
6492    styleSheet.type = "text/css";
6493    styleSheet.innerText = styles;
6494    document.head.appendChild(styleSheet);
6495
6496    if (typeof window.SwymStorefrontLayoutContext === 'undefined') {
6497        window.SwymStorefrontLayoutContext = {};
6498    }
6499    if (typeof window.SwymStorefrontLayoutExtensions === 'undefined'){
6500        window.SwymStorefrontLayoutExtensions = {};
6501    }
6502
6503    // Expose block settings toggle state so ThemeCSSMapping can respect it.
6504    // When true, theme editor color pickers take priority over inherited theme CSS variables.
6505    SwymStorefrontLayoutContext.BlockSettingsEnabled = isControlCentreEnabledFromBlockSettings;
6506
6507    if (isControlCentreEnabledFromBlockSettings) {
6508      // If block settings are enabled, use them
6509      SwymStorefrontLayoutContext.Settings = {
6510        EnableStorefrontLayoutOnLauncher: true,
6511        EnableStorefrontLayoutNotification: window?.SwymWishlistCommonCustomizationSettings?.enableStorefrontLayoutNotification ?? true,
6512        StorefrontLayoutType: window?.SwymWishlistCommonCustomizationSettings?.storefrontLayoutType || "as-drawer",
6513        StorefrontLayoutDrawerPosition: "left",
6514        StorefrontLayoutAsSectionContainerId: "swym-wishlist-render-container",
6515        StorefrontLayoutAsSectionPageURL: "/pages/swym-wishlist",
6516        EnableStorefrontLayoutVariantSelector: true,
6517        StorefrontLayoutNotificationPosition: "left",
6518        StorefrontLayoutActionPopupPosition: "left",
6519        StorefrontLayoutNotificationDuration: 5000
6520      };
6521    } else {
6522      // If only metafield is enabled but no block settings, use common settings
6523      SwymStorefrontLayoutContext.Settings = {
6524        EnableStorefrontLayoutOnLauncher: true,
6525        EnableStorefrontLayoutNotification: window?.SwymWishlistCommonCustomizationSettings?.enableStorefrontLayoutNotification ?? true,
6526        StorefrontLayoutType: window?.SwymWishlistCommonCustomizationSettings?.storefrontLayoutType || "as-drawer",
6527        StorefrontLayoutDrawerPosition: "left",
6528        StorefrontLayoutAsSectionContainerId: "swym-wishlist-render-container",
6529        StorefrontLayoutAsSectionPageURL: "/pages/swym-wishlist",
6530        EnableStorefrontLayoutVariantSelector: true,
6531        StorefrontLayoutNotificationPosition: "left",
6532        StorefrontLayoutActionPopupPosition: "left",
6533        StorefrontLayoutNotificationDuration: 5000
6534      };
6535    }
6536    const storefrontLayoutCallback = (swat) =>{
6537      SwymStorefrontLayoutContext.swat = swat;
6538      let isStoreOnPaidPlan = swat.getApp('Wishlist')?.['is-paid'];
6539      let isWishlistEnabled = swat.getApp('Wishlist')?.['enabled'];
6540      SwymStorefrontLayoutContext.Settings = {
6541        ...SwymStorefrontLayoutContext?.Settings,
6542        EnableStorefrontLayoutCollection: window?.SwymEnabledCommonFeatures?.["multiple-wishlist"] ?? false,
6543        EnableStorefrontLayout: isWishlistEnabled && (isControlCentreEnabledFromBlockSettings || isControlCentreEnabledFromMetafield),
6544      }
6545
6546      if(SwymStorefrontLayoutContext?.Settings?.EnableStorefrontLayout){
6547        if(SwymStorefrontLayoutContext?.CustomEvents?.LayoutInitialized){
6548          var event = new CustomEvent(SwymStorefrontLayoutContext.CustomEvents.LayoutInitialized, { 
6549            detail: { settings: SwymStorefrontLayoutContext?.Settings }
6550          });
6551          document.dispatchEvent(event);
6552        }else{
6553          swat.utils.warn(`LayoutInitialized event is not defined.`);
6554        }
6555      }else{
6556        swat.utils.warn(`Storefront Layout is disabled.`);
6557        document.getElementById("swym-storefront-layout-container")?.remove();
6558        document.getElementById("swym-storefront-extention-render-container")?.remove();
6559      }
6560    }
6561  
6562    if (!window.SwymCallbacks) {
6563      window.SwymCallbacks = [];
6564    }
6565    window.SwymCallbacks.push(storefrontLayoutCallback);
6566  
6567    SwymStorefrontLayoutContext.SwymCustomerData = {
6568      
6569        name: null,
6570        email: null
6571      
6572    };
6573    SwymStorefrontLayoutContext.isShopperLoggedIn = !!SwymStorefrontLayoutContext?.SwymCustomerData?.email;
6574  })();
6575</script>
6575
6576
6577
6578
6579
6580  
6581    <div id="swym-storefront-extention-render-container">
6582      <!-- BEGIN app snippet: swymStorefrontLayout -->
6582<script>
6583    (function () {
6584        const isControlCentreEnabledFromMetafield = window?.SwymEnabledCommonFeatures?.["control-centre"];
6585        const isControlCentreEnabledFromBlockSettings = true;
6586        const isCommonCustomizationEnabledFromMetafield = window?.SwymWishlistCommonCustomizationSettings && 
6587            Object.keys(window.SwymWishlistCommonCustomizationSettings).length > 0;
6588        
6589        if (!(isControlCentreEnabledFromMetafield && isCommonCustomizationEnabledFromMetafield) && !isControlCentreEnabledFromBlockSettings) {
6590            return;
6591        }
6592        if (typeof window.SwymStorefrontLayoutContext === 'undefined') {
6593            window.SwymStorefrontLayoutContext = {};
6594        }
6595        
6596        // Initialize strings with empty values first to prevent string changes after loadStrings()
6597        SwymStorefrontLayoutContext.Strings = {
6598            title: "",
6599            addToCart: "",
6600            addedToCart: "",
6601            addAllToCartCta: "",
6602            addAllToCartSuccessText: "",
6603            addAllToCartEmptyText: "",
6604            addAllToCartErrorText: "",
6605            soldOut: "",
6606            addingToCart: "",
6607            viewCartCta: "",
6608            moveToCartCta: "",
6609            movingToCartCta: "",
6610            removeSflItemCta: "",
6611
6612            loginHeading: "",
6613            loginText: "",
6614            loginButtonText: "",
6615            loggedUserWelcomeMessage: "",
6616            VariantSelectorBtnText: "",
6617            wishlistTitle: "",
6618            wishlistInfo: "",
6619            emptyWishlistTitle: "",
6620            emptyWishlistDescription: "",
6621            
6622            collectionTitle: "",
6623            emptyCarouselCollectionText: "",
6624            emptyCollectionText: "",
6625            emptyCollectionDescription: "",
6626            
6627            addToCollectionTitle: "",
6628            removeItemCta: "",
6629            addToCollectionCta: "",
6630            createCollectionCta: "",
6631            saveNewCollectionCta: "",
6632            renameCollectionCta: "",
6633            deleteCollectionCta: "",
6634            shareCollectionCta: "",
6635            saveCollectionCta: "",
6636            editCollectionCta: "",
6637            shareCollectionTitle: "",
6638            shareCollectionMessage: "",
6639            sharedCollectionMessage: "",
6640            updateCollectionTitle: "",
6641
6642            errorMessageListNameRequired: "",
6643            errorMessageListNameRequire3Char: "",
6644            errorMessageListNameAlreadyExist: "",
6645
6646            notificationMessageItemSaved: "",
6647            notificationMessageItemRemoved: "",
6648            notificationMessageAddedToCart: "",
6649            notificationMessageAddedToCollection: "",
6650            notificationMessageCollectionSaved: "",
6651            notificationMessageCollectionDeleted: "",
6652            notificationMessageCollectionUpdated: "",
6653            notificationMessageCollectionUnavailable: "",
6654            notificationMessageSFLItemSaved: "",
6655            notificationMessageSFLItemRemoved: "",
6656            notificationMessageMovedToCart: "",
6657
6658            notificationActionAddToCollection: "",
6659            notificationActionView: "",
6660            notificationActionViewCollection: "",
6661            notificationActionGoToCart: "",
6662
6663            item: "",
6664            items: "",
6665
6666            tabWishlist: "",
6667            tabSavedForLater: "",
6668            tabSavedForYou: "",
6669            
6670            savedForLaterTitle: "",
6671            emptySavedForLaterTitle: "",
6672            emptySavedForLaterDescription: "",
6673
6674            awNudgeMessage: "",
6675            awNudgeMessageSub: "",
6676            awNudgeLoginCTA: "",
6677            awNudgeDismiss: "",
6678
6679            emptySingleLoggedInTitle: "",
6680            emptySingleLoggedInDescription: "",
6681            emptySingleLoggedInBullet1: "",
6682            emptySingleLoggedInBullet2: "",
6683            emptySingleLoggedInBullet3: "",
6684            emptySingleLoggedInCTA: "",
6685
6686            emptyMultiLoggedInTitle: "",
6687            emptyMultiLoggedInDescription: "",
6688            emptyMultiLoggedInCreateTitle: "",
6689            emptyMultiLoggedInCreateDescription: "",
6690            emptyMultiLoggedInBrowseLabel: "",
6691            emptyMultiLoggedInCTA: "",
6692            emptyMultiLoggedInTag1: "",
6693            emptyMultiLoggedInTag2: "",
6694            emptyMultiLoggedInTag3: "",
6695            emptyMultiLoggedInTag4: "",
6696            emptyMultiLoggedInTag5: "",
6697
6698            emptyGuestTitle: "",
6699            emptyGuestDescription: "",
6700            emptyGuestSingleNotice: "",
6701            emptyGuestMultiNotice: "",
6702            emptyGuestLoginCTA: "",
6703            emptyGuestBrowseCTA: "",
6704            emptyGuestSignupText: "",
6705            emptyGuestSignupCTA: "",
6706
6707            emptySflTitle: "",
6708            emptySflDescription: "",
6709            emptySflStep1Title: "",
6710            emptySflStep1Description: "",
6711            emptySflStep2Title: "",
6712            emptySflStep2Description: "",
6713            emptySflLoggedInNotice: "",
6714            emptySflGuestNotice: "",
6715            emptySflGoToCartCTA: "",
6716            emptySflLoginCTA: "",
6717            emptySflSignupText: "",
6718            emptySflSignupCTA: ""
6719        };
6720
6721        SwymStorefrontLayoutContext.stringsLoaded = false;
6722        SwymStorefrontLayoutContext.StorefrontLayoutUrls = SwymStorefrontLayoutContext.StorefrontLayoutUrls || {};
6723        SwymStorefrontLayoutContext.StorefrontLayoutUrls.Login = "\/customer_authentication\/login?return_to=%2Fproducts%2Fiptg\u0026locale=en\u0026ui_hint=full\u0026region_country=US";
6724
6725        /** 
6726        * Function to override Swym's default UI behavior.
6727        **/
6728        const overrideSwymDefaultUI = (swat) => {
6729            try{
6730                swat.ui.open = function(){
6731                    window.location.hash = SwymStorefrontLayoutContext?.StorefrontLayoutUrls?.List;
6732                }
6733
6734                if (swat.ui?.uiRef?.settings?.UI) {
6735                    swat.ui.uiRef.settings.UI.NotificationDisabled = true;
6736                }
6737            }catch(e){
6738                swat.utils?.warn('Error overriding Swym default UI:', e);
6739            }
6740        }
6741
6742        /**
6743        * Callback function to override Swym's default UI behavior based on Storefront Layout settings.
6744        * 
6745        * - Overri
6745des `swat.ui.open` to redirect to a custom Storefront Layout URL if enabled.
6746        * - Overrides Swym's default notifications to log notifications instead of displaying them.
6747        * - Ensures the callback is pushed to `window.SwymCallbacks` for execution when Swym is initialized.
6748        * 
6749        * @param {Object} swat - Swym's UI object used for overriding UI behaviors.
6750        */
6751        const swymStorefrontLayoutCallback = (swat) =>{
6752            SwymStorefrontLayoutContext.swat = swat;
6753            
6754            overrideSwymDefaultUI(swat);
6755
6756
6757            let triggerHeartbeat = swat?.ExtensionHealth?.triggerExtensionHeartbeat;
6758            if (typeof triggerHeartbeat !== "function") return;
6759
6760            let wishlistNewUiHeartbeatData = '';
6761            try {
6762                wishlistNewUiHeartbeatData = JSON.parse(wishlistNewUiHeartbeatData) || {};
6763            } catch (e) {
6764                wishlistNewUiHeartbeatData = {};
6765            }
6766
6767            let themeId = window.Shopify.theme.id;
6768            let isDesignMode = !!window.swymDesignMode;
6769            let lastHeartbeat = isDesignMode ? wishlistNewUiHeartbeatData?.editor : wishlistNewUiHeartbeatData?.[themeId];
6770            let themeContext = isDesignMode ? 'editor' : themeId;
6771            let extensionData = {
6772                extensionName: "wishlist-new-ui", 
6773                extensionType: "app-embed", 
6774                metadata: {}, 
6775                themeId: themeContext, 
6776                extensionSource: "default"
6777            };
6778            /** Stop heartbeat
6779            triggerHeartbeat(extensionData, lastHeartbeat);
6780            */
6781        }
6782        
6783        if (!window.SwymCallbacks) {
6784            window.SwymCallbacks = [];
6785        }
6786        window.SwymCallbacks.push(swymStorefrontLayoutCallback);
6787    })();
6788</script>
6788
6789<link rel="stylesheet" href="https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/storefront-layout.css">
6790<script src="https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/async-wishlist-apis.js" defer="defer"></script>
vendor: 1 bytes, line 6790
6790
6791<script src="https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/storefront-layout.js" defer="defer"></script>
vendor: 1 bytes, line 6791
6791
6792<script src="https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/storefront-layout-components.js" defer="defer"></script>
6792
6793
6794<swym-storefront-layout class="swym-storefront-layout-root-component"></swym-storefront-layout>
6795<!-- END app snippet -->
6796    </div>
6797    <!-- BEGIN app snippet: swymEducationTooltip -->
6798
6799
6800  
6801
6802  <div id="swym-education-tooltip" aria-hidden="true"
6803    data-toast-desktop-message="Interesting? Click &#39;{{wishlistIcon}}&#39; to save it to your {{wishlistName}}."
6804    data-toast-mobile-message="Interesting? Tap &#39;{{wishlistIcon}}&#39; to add to your {{wishlistName}}."
6805    data-toast-cta="Wishlist Now"
6806    data-tooltip-desktop-message="Interesting? Click &#39;{{wishlistIcon}}&#39; to save it to your {{wishlistName}}."
6807    data-tooltip-mobile-message="Interesting? Tap &#39;{{wishlistIcon}}&#39; to add to your {{wishlistName}}."
6808    data-button-transition-text-1="Price drops? Know first."
6809    data-button-transition-text-2="Almost gone. Just saying."
6810    data-button-transition-text-3="Yours when ready.">
6811  </div>
6812
6813  <div id="swym-education-tooltip-bubble" class="swym-education-tooltip swym-education-tooltip-above" aria-hidden="true" role="tooltip">
6814    <div class="swym-education-tooltip-content">
6815      <span class="swym-education-tooltip-message"></span>
6816      <button class="swym-education-tooltip-close" data-swym-education-tooltip-close aria-label="Close consent dialog">
6817        <svg width="12" height="12" viewBox="0 0 24 24" fill="none" stroke="currentColor" stroke-width="2">
6818          <path d="M18 6L6 18M6 6l12 12"></path>
6819        </svg>
6820      </button>
6821    </div>
6822    <div class="swym-education-tooltip-arrow"></div>
6823  </div>
6824
6825  <style>
6826    /* Education tooltip — positioned fixed, managed by JS */
6827    .swym-education-tooltip {
6828      position: fixed;
6829      z-index: 500;
6830      visibility: hidden;
6831      opacity: 0;
6832      transition: opacity 0.2s ease, visibility 0.2s ease;
6833      pointer-events: none;
6834    }
6835    .swym-education-tooltip.swym-education-tooltip-visible {
6836      visibility: visible;
6837      opacity: 1;
6838      pointer-events: auto;
6839    }
6840    .swym-education-tooltip-content {
6841      background: var(--swym-storefront-layout-notification-neutral-bg-color, #FFFFFF);
6842      color: var(--swym-storefront-layout-notification-neutral-text-color, #383d41);
6843      border: 1px solid var(--swym-storefront-layout-notification-neutral-border-color, #d6d8db);
6844      padding: 10px 14px;
6845      border-radius: var(--swym-storefront-layout-ui-border-radius, 10px);
6846      font-size: 13px;
6847      line-height: 1.4;
6848      display: flex;
6849      align-items: center;
6850      gap: 8px;
6851      max-width: 320px;
6852      box-shadow: 0 2px 8px rgba(0, 0, 0, 0.2);
6853    }
6854    .swym-education-tooltip-message {
6855      flex: 1;
6856    }
6857    .swym-education-tooltip-close {
6858      background: none;
6859      border: none;
6860      color: inherit;
6861      cursor: pointer;
6862      padding: 2px;
6863      line-height: 1;
6864      flex-shrink: 0;
6865      opacity: 0.7;
6866    }
6867    .swym-education-tooltip-close:hover {
6868      opacity: 1;
6869    }
6870
6871    /* Button text fade transition for education nudge */
6872    .swym-btn-text-fade-out {
6873      opacity: 0;
6874      transition: opacity 0.2s ease;
6875    }
6876    .swym-btn-text-fade-in {
6877      opacity: 1;
6878      transition: opacity 0.3s ease;
6879    }
6880
6881    /* Arrow — points down when tooltip is above, up when below */
6882    .swym-education-tooltip-arrow {
6883      width: 0;
6884      height: 0;
6885      position: absolute;
6886      left: 50%;
6887      transform: translateX(-50%);
6888    }
6889    .swym-education-tooltip-above .swym-education-tooltip-arrow {
6890      bottom: -6px;
6891      border-left: 6px solid transparent;
6892      border-right: 6px solid transparent;
6893      border-top: 6px solid var(--swym-storefront-layout-notification-neutral-bg-color, #FFFFFF);
6894    }
6895    .swym-education-tooltip-below .swym-education-tooltip-arrow {
6896      top: -6px;
6897      border-left: 6px solid transparent;
6898      border-right: 6px solid transparent;
6899      border-bottom: 6px solid var(--swym-storefront-layout-notification-neutral-bg-color, #FFFFFF);
6900    }
6901
6902    /* Hidden data element */
6903    #swym-education-tooltip {
6904      display: none;
6905    }
6906
6907    @media (max-width: 480px) {
6908      .swym-education-tooltip-content {
6909        max-width: 90vw;
6910        font-size: 12px;
6911      }
6912    }
6913  </style>
6914
6915  
6915<script src="https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/swym-education-tooltip.js" defer="defer"></script>
6915
6916  
6916<script src="https://cdn.shopify.com/extensions/01a0f142-9ec3-73a2-babd-8904a7e44cee/swym-relay-840/assets/swym-education.js" defer="defer"></script>
6916
6917
6918<!-- END app snippet -->
6919  
6920
6921</div><div id="shopify-block-AUmU0akhDazRra1h5V__9246707248847178381" class="shopify-block shopify-app-block">
6922  
6922<script src="https://shopify.livechatinc.com/api/v1/script/shop/9t079h-bv.myshopify.com/widget.js" defer></script>
6922
6923
6924
6925
6926</div><div id="shopify-block-AbEpqaklGb2U2U255a__10274264662263595587" class="shopify-block shopify-app-block">
6927<script>
6928  window.SwymCollectionsConfig = {
6929  'CollectionsSelectors': [],
6930  'CollectionsPosition': "top-right",
6931  'CollectionsIcon': "heart",
6932  'CollectionsButtonSize': 25,
6933  'CollectionsVerticalOffset': 0,
6934  'CollectionsHorizontalOffset': 0,
6935  'CollectionsProductLinkSelector': "-", 
6936  'CollectionsProductTileSelector': "-",
6937  'CollectionsVariantSelector': "false",
6938  'CollectionsVariantSelectorChooseActionText': "Choose or Create new Wishlist",
6939  'CollectionsCreateListTitle': "Name Your Wishlist",
6940  'CollectionsCreateListPlaceholder': "Enter wishlist name",
6941  'CollectionsCustomIconAdd': "",
6942  'CollectionsCustomIconAdded': "",
6943  'CollectionsIconColor': "#000000",
6944  'CollectionsIconThickness': "1.7",
6945}
6946</script>
6946
6947<style>
6948  .swym-wishlist-collections-v2, .swym-wishlist-collections-v2 svg {border:unset; background:unset; min-width: 25px; height: 25px!important;}
6949  .swym-wishlist-collections-icon-heart-filled, .swym-wishlist-collections-icon-heart-unfilled, .swym-wishlist-collections-icon-star-filled, .swym-wishlist-collections-icon-star-unfilled, .swym-wishlist-collections-icon-bookmark-unfilled, .swym-wishlist-collections-icon-bookmark-filled {fill: #000000;}
6950  .swym-wishlist-collections-v2 .swym-collections-social-count {color: #000000;}
6951</style>
6952
6953
6954
6955<script>
6956  // Send heartbeat for collections button
6957  (function() {
6958    let collectionsButtonHeartbeatData = '';
6959    try {
6960      collectionsButtonHeartbeatData = JSON.parse(collectionsButtonHeartbeatData) || {};
6961    } catch (e) {
6962      collectionsButtonHeartbeatData = {};
6963    }
6964    const ShopifyTheme = window.Shopify.theme;
6965    const themeId = ShopifyTheme.id;
6966    let heartbeatMetadata = {
6967      schema_name: ShopifyTheme?.schema_name,
6968      schema_version: ShopifyTheme?.schema_version,
6969      theme_store_id: ShopifyTheme?.theme_store_id,
6970      role: ShopifyTheme?.role
6971    };
6972
6973    const isDesignMode = !!window.swymDesignMode;
6974
6975    if (!window.SwymCallbacks) {
6976      window.SwymCallbacks = [];
6977    }
6978    window.SwymCallbacks.push((swat) => {
6979      if (!swat || !themeId) {
6980        return;
6981      }
6982      const triggerHeartbeat = swat?.ExtensionHealth?.triggerExtensionHeartbeat;
6983      if (typeof triggerHeartbeat !== "function") return;
6984
6985      const lastHeartbeat = isDesignMode ? collectionsButtonHeartbeatData?.editor : collectionsButtonHeartbeatData?.[themeId];
6986      const themeContext = isDesignMode ? 'editor' : themeId;
6987
6988      const extensionData = {
6989        extensionName: "add-to-wishlist-collections-button", 
6990        extensionType: "app-embed", 
6991        metadata: heartbeatMetadata, 
6992        themeId: themeContext, 
6993        extensionSource: "default"
6994      };
6995
6996      /** Stop heartbeat
6997      triggerHeartbeat(extensionData, lastHeartbeat);
6998      */
6999    });
7000  })(); // IIFE to prevent polluting global scope
7001</script>
7001
7002
7003
7004<style>
7005  .swym-advanced-wishlist-collections-container {
7006    display: none;
7007  }
7008</style>
7009</div>
7009<script src="https://cdn.shopify.com/storefront/standard-actions.js" type="module" data-source-attribution="shopify.standard_actions"></script>
7009
7010</body>
7011</html>

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